Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyzer.

Watanabe, Nozomi; Kamei, Shigeru; Ohkubo, Akihiro; Yamanaka, Manabu D.; Ohsawa, Seiji; Makino, Kumiko; Tokuda, K

doi:10.1093/clinchem/32.8.1551

Cited by 400 publications

(114 citation statements)

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“…The extracts were thawed 1 h before performance of the skin tests and mixed by Vortex. The protein concentration of each in-house extract was determined using the method of Watanabe et al [32] The protein concentration of the lupine and peanut extract was 1.82 and 1.22 g/L. respectively.…”

Section: Preparation Of In-house Food Extractsmentioning

confidence: 99%

Sensitization to lupine flour: is it clinically relevant?

Jong

Maaren

Vlieg-Boersta

et al. 2010

Clin Experimental Allergy

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These results demonstrate that clinical lupine allergy is very uncommon, even in the presence of sensitization to lupine flour. The estimated prevalence of lupine allergy, among patients with a suspected food allergy, referred to a tertiary allergy centre in the Netherlands is 0.27-0.81%. In most, although not all cases, sensitization is not clinically relevant and is most likely caused by cross-sensitization to peanut. In selected cases, eliciting doses are low, making significant reactions possible.

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Section: Preparation Of In-house Food Extractsmentioning

confidence: 99%

Sensitization to lupine flour: is it clinically relevant?

Jong

Maaren

Vlieg-Boersta

et al. 2010

Clin Experimental Allergy

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“…To determine total LDL concentration, LDL cholesterol, phospholipids and triacylglycerols were assayed by enzymatic methods [27][28][29]. Protein concentration in LDL was determined by a pyrogallol red technique (Elitech Diagnostics, Sees, France) [30]. Aqueous solutions of melatonin or GWC22 were added to the LDL in order to obtain a final compound concentration of 10 )4 m. Melatonin partitioning between LDL and aqueous phases has been determined after several extraction steps.…”

Section: Preparation Of Ldl and Quantification Of Melatonin In Ldlmentioning

confidence: 99%

Quantification of the water/lipid affinity of melatonin and a pinoline derivative in lipid models

Mekhloufi¹,

Vitrac²,

Yous³

et al. 2007

Journal of Pineal Research

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This study assessed the location of melatonin (N-acetyl-5-methoxytryptamine) and of a pinoline derivative (GWC22) [6-ethyl-1-(3-methoxyphenyl)-2-propyl-1,2,3,4-tetrahydro-beta-carboline], when present in lipid assemblies such as linoleate micelles, phosphatidylcholine liposomes or low density lipoproteins (LDL). The efficiency of radical scavenging by these compounds is highly dependent on their partitioning between the lipidic and aqueous phases. We determined the proportion of melatonin or GWC22 in the aqueous and lipid phases of each system (concentrations of the antioxidants ranging between 3 x 10(-5) and 10(-4) m) by assaying melatonin or GWC22 by HPLC/UV detection, or by fluorescence for melatonin in micelles. Our results show that melatonin and GWC22 were preferentially located in the aqueous phase of micelles (68.4% and 59.0%, respectively), whereas only 30.5% of melatonin and 39.0% of GWC22 were found in the lipid phase. By contrast, in phosphatidylcholine liposomes, both compounds were essentially present in the lipid phase (73.5% for melatonin and 79.1% for GWC22, versus 25.9% and 19.5% in the aqueous phase, respectively). In the case of LDL, 99.9% of the melatonin added was found in the methanol/water extracting phase containing phospholipids, unesterified cholesterol and apolipoprotein B100. The partitioning of melatonin and GWC22 in linoleate micelles gave new insights on the marked protective effect of GWC22 towards radiation-induced lipid peroxidation and allowed us to determine more accurately the lower limit values of the reaction rate constants of the two molecules studied with lipid peroxyl radicals, i.e. k(LOO.+melatonin)) >or= 9.0 x 10(4)m(-1)s(-1) and k(LOO.+GWC22) >or= 3.5 x 10(5)m(-1)s(-1).

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“…Cholesterol, choline phospholipids and triacylglycerols in LDLs were assayed by enzymatic methods [38][39][40] and protein concentration in LDLs was determined by a pyrogallol red technique (Elitech Diagnostics, Sees, France) [41] to determine total LDL concentration. For the radiolysis experiments, the dialyzed solution of LDLs was adjusted to a concentration of 3 g/L (expressed as total LDLs), by dilution in 10 mmol/L sodium phosphate buffer (pH 7).…”

Section: Preparation Of Ldlsmentioning

confidence: 99%

Melatonin related compounds inhibit lipid peroxidation during copper or free radical‐induced LDL oxidation

Bonnefont‐Rousselot

Chevé

Gozzo

et al. 2002

Journal of Pineal Research

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This study was designed to evaluate the protective effect of two melatonin related compounds towards low density lipoproteins (LDL) oxidation initiated in vitro either by defined free radicals [i.e. superoxide anion (O2*-) and ethanol-derived peroxyl radicals (RO(2)(*))] produced by gamma radiolysis or by copper ions. The compounds studied were N-[2-(5-methoxy-1H-indol-3-yl)ethyl]-3,5-di-tert-butyl-4-hydroxybenzamide (DTBHB) and (R,S)-1-(3-methoxyphenyl)-2-propyl-1,2,3,4-tetrahydro-beta-carboline (GWC20) which is a pinoline derivative. Their effects were compared with those of melatonin at the same concentration (100 micromol/L). None of the three tested compounds protected endogenous LDL alpha-tocopherol from oxidation by RO(2)(*)/O(2)(*)- free radicals. By contrast, they all protected beta-carotene from the attack of these free radicals with GWC20 being the strongest protector. Moreover, melatonin and DTBHB partially inhibited the formation of products derived from lipid peroxidation (conjugated dienes and thiobarbituric acid-reactive substances or TBARS) while GWC20 totally abolished this production. As previously shown, melatonin (at the concentration used) inhibited copper-induced LDL oxidation by increasing 1.60-fold the lag phase duration of conjugated diene formation over the 8 hr of the experimental procedure, however, DTBHB and GWC20 were much more effective, because they totally prevented the initiation of the propagation phase of LDL oxidation. It would be interesting to test in vivo if DTBHB and GWC20 which exhibit a strong capacity to inhibit in vitro LDL oxidation would reduce or not atherosclerosis in animals susceptible to this pathology.

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Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyzer.

Cited by 400 publications

References 0 publications

Sensitization to lupine flour: is it clinically relevant?

Sensitization to lupine flour: is it clinically relevant?

Quantification of the water/lipid affinity of melatonin and a pinoline derivative in lipid models

Melatonin related compounds inhibit lipid peroxidation during copper or free radical‐induced LDL oxidation

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