Urinary proteome analysis of acute hypercoagulable state in rat model induced by ε-aminocaproic acid

Jing, Jian; Du, Zhenhuan; Ji, Songyang; Han, Ke

doi:10.1016/j.biopha.2018.11.148

Cited by 5 publications

(2 citation statements)

References 53 publications

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“…These results suggest that the ETSL-induced hypercoagulable state has a certain effect on rat kidneys, but this effect is far less intense than acute kidney injury. Surprisingly, we found that the hypercoagulable state of the body may not involve direct changes in coagulation factors in vivo but causes changes in the upstream regulatory points of the coagulation cascade system, consistent with our previous studies (Jing et al, 2019a;Jing et al, 2019b). Upon further stimuli, the coagulation cascade system is triggered and eventually leads to the development of thrombus.…”

Section: Discussionsupporting

confidence: 92%

“…During the hypercoagulable state, changes in protein expression or protein content in the kidney and digestive and reproductive systems were significant. Furthermore, we compared the urinary differential protein profile of the ETSL-induced hypercoagulable state with those of the other two types of druginduced hypercoagulable states (Jing et al, 2019a;Jing et al, 2019b). As shown in Table 3 and Figure 6, we found no common differential proteins in the acute hypercoagulable state induced by three classes of drugs: ETSL, TXA, or EACA.…”

Section: Discussionmentioning

confidence: 87%

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Proteome Analysis of Urinary Biomarkers in Acute Hypercoagulable State Rat Model

Jing

Qin

2021

Front. Mol. Biosci.

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Thrombotic diseases are usually preceded by a hypercoagulable state in the body. This study aimed to screen potential urinary biomarkers for hypercoagulable state based on proteome analysis. Wistar rats were administered with the hemostatic agent etamsylate to establish hypercoagulable state. Urine samples were collected for proteome analysis. We found 20 proteins with levels more than 1.5-fold in difference between control rats and model rats. We searched human homologs of 20 rat proteins and identified 13 human proteins. Of the 13 human homologous proteins, nine were members of human core urinary proteome. Human homologous proteins of differential proteins were highly expressed in 31 human tissues, especially in the kidneys followed by digestive system and reproductive system. Surprisingly, we did not identify known coagulation factors as differential proteins in the urine of model rats. Hypercoagulable state of the body may not involve direct changes in coagulation factors but causes the changes upstream of the coagulation cascade system. Common differential urinary proteins between different hypercoagulable states suggest some common pathways in the formation of hypercoagulable states and may serve as potential biomarkers for the prevention and treatment of thrombotic diseases.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 87%