Urine, feces, and blood contamination of frozen and fresh tambaqui (Colossoma macropomum Cuvier, 1818) sperm

Marques, Lis Santos; Gracia, Luis Fernando Guerrero; Pérez-Atehortúa, Maritza; Rodrigues, Rômulo Batista; Teixeira, Nathalia dos Santos; Oberst, Eneder Rosana; Streit, Danilo Pedro

doi:10.1016/j.cryobiol.2021.07.003

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…Additionally, we showed that the level of blood contamination was higher in the PESA approach, which might be due to mechanical disruption of the regional blood vessels during needle movements. Earlier studies have shown that blood contamination had negative effects on both fresh and frozen–thawed spermatozoa (Marques et al, 2021). Previous researchers (Varesi et al, 2013a; Varesi et al, 2013b) conducted a study on the quality of canine spermatozoa retrieved by PESA and compared it with in vitro aspiration (comparable with the MESA procedure we applied in the current research) and epididymal mincing methods.…”

Section: Discussionmentioning

confidence: 99%

Effects of epididymal sperm recovery methods on fresh and frozen–thawed sperm characteristics in dogs

Mogheiseh

Divar

Vesal

et al. 2022

Reprod Domestic Animals

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The cauda epididymis holds a collectible source of fertile spermatozoa in cases of obstructive azoospermia, sudden death, and after elective or emergency castration. The current study was conducted to compare three different epidydimal sperm collection methods (Percutaneous epididymal sperm aspiration (PESA), microsurgical epididymal sperm aspiration (MESA) and retrograde epididymal wash (EW)) in the dog. Fifteen large‐breed adult dogs were applied for comparing the PESA (left testicles) with MESA (right testicles) techniques, while five dogs were used for evaluation of MESA (left testicles) versus EW (right testicles). The recovered sperm cells from MESA and EW were subjected to cryopreservation. Total sperm recovery, level of blood contamination and sperm quality markers (viability, morphology, plasma and acrosome membrane integrity, DNA fragmentation, and metabolic activity) were evaluated for fresh and frozen–thawed spermatozoa. We showed that the collection of epididymal sperm cells through the PESA method resulted in lower total sperm recovery and significantly reduced fresh sperm kinematic and quality measures. While, both MESA and EW procedures resulted in a high number of intact epididymal spermatozoa with appropriate cryo‐tolerance potential. In conclusion, EW and MESA methods provide high‐quality epidydimal spermatozoa with high cryopreservation potential in domestic dogs.

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Section: Discussionmentioning

confidence: 99%

Effects of epididymal sperm recovery methods on fresh and frozen–thawed sperm characteristics in dogs

Mogheiseh

Divar

Vesal

et al. 2022

Reprod Domestic Animals

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“…Before the gametes collection, the urogenital area was cleaned and dried to avoid contamination by mucus or water. The first sperm-released samples were discarded to prevent contamination with urine, feces, or blood (Marques et al, 2021). The sperm samples of each animal were collected in dry graduated plastic tubes through anteroposterior massage in the ventral region of the fish.…”

Section: Fish Handling and Gametes Collectionmentioning

confidence: 99%

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

de Souza França

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Fish sperm cryopreservation is a technique that can increase the reproduction in captive efficiency of freshwater and marine fishes. The technique is applied in environmental conservation programs and aquaculture. Over the last few decades, protocols for sperm cryopreservation from many fishes have been established. However, the researchers' main focus was successfully freezing and thawing sperm, neglecting the period in which the gametes remain in contact with the cryoprotective solution until fertilization. Exposure of sperm to cryoprotectant solutions after thawing can be harmful to the quality of the gametes since they can be toxic.The vast majority of the established protocols use ultra-resistant plastic containers to store sperm during the cryopreservation process. These containers usually are not reused, generating highly polluting waste for the environment. Furthermore, in some countries, the containers usually used are sold by a few industries, which makes acquisition difficult and increases the product's price. Thus, the main objective of the thesis was to create and test low-cost methodologies that enhance the use of fish post-thaw sperm and make the sperm cryopreservation process more environmentally friendly.The experiments in the Chapters 1 and 2 were developed in Brazil. We used the South American silver catfish Rhamdia quelen, a species considered an experimental model for native South American fishes. In vitro fertilization and sperm cryopreservation protocols for the species were already established; thus, we based our work on these protocols to apply innovations.In Chapter 1, we tested the use of post-thawing dilution to reduce the toxicity of the cryoprotectant solution. This technique is commonly used in mammalian sperm cryopreservation protocols but has never before been applied to post-thaw sperm of South American fishes. South American silver catfish post-thaw sperm samples were diluted in a saline extender (1.1% NaCl -325 mOsm kg -1 ; pH 7.6). The post-thaw sperm diluted samples showed higher velocities, straightness, progression, and flagellar beat frequency than SUMMARY 2 the cells of undiluted samples (control). The post-thawing dilution also provided higher fertilization and hatching rates than the control group. Thus, the post-thawing sperm dilution proved to be a simple, cheap, and efficient methodology that should be included in the silver catfish sperm cryopreservation protocol.

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The use of differential separation and density gradient with AllGrad 90% after thawing improves the sperm quality of South American catfish (Rhamdia quelen)

et al. 2022

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Urine, feces, and blood contamination of frozen and fresh tambaqui (Colossoma macropomum Cuvier, 1818) sperm

Cited by 4 publications

References 36 publications

Effects of epididymal sperm recovery methods on fresh and frozen–thawed sperm characteristics in dogs

Effects of epididymal sperm recovery methods on fresh and frozen–thawed sperm characteristics in dogs

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

The use of differential separation and density gradient with AllGrad 90% after thawing improves the sperm quality of South American catfish (Rhamdia quelen)

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