Urine miRNA as a potential biomarker for bladder cancer detection – a meta-analysis

Kutwin, Piotr; Konecki, Tomasz; Borkowska, Edyta; Traczyk-Borszynska, M.; Jabłonowski, Zbigniew

doi:10.5173/ceju.2018.1605

Cited by 20 publications

(9 citation statements)

References 42 publications

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“…Since miRNAs can be detected in a huge variety of bodily fluids including urine and since miRNAs are stable in urine, urine seems like a promising non-invasive test for the detection of EC [65]. Urinary miRNAs have shown potential in the detection of bladder and prostate cancer [66,67], however in EC only one study has used urine for the detection of miRNA [26]. In addition, Zavesky et al compared urinary miRNA expression levels of preand post-surgery ovarian cancer samples and between patients with ovarian and endometrial cancer (n = 10) and healthy controls and proposed urinary miRNA should be further investigated to test the diagnostic potential of urine miRNAs in gynaecological cancers [68].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of microRNA panel in endometrial cancer: A systematic review

2020

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Purpose: We conducted a systematic review to evaluate the overall diagnostic accuracy of miRNAs in detecting endometrial cancer. Materials and Methods: A systematic search of Medline, Embase, Cinahl and the Cochrane Controlled Register of Trials was performed to identify studies reporting on the diagnostic value of miRNA in EC patients. Included were diagnostic studies looking at miRNA expression in women diagnosed with endometrial cancer. Two reviewers independently selected studies and assessed quality of studies using the Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2) score system. Data extraction was completed and the vote-counting strategy was used to rank miRNAs. Results: 26 studies were included with a total number of 1,400 EC patients reporting on 106 differentially expressed miRNAs. The most frequently found upregulated miRNA was miR-205 followed by miR-200c,-223,-182,-183 and-200a. In addition, miR-135b, miR-429, miR-141 and miR-200b were also frequently upregulated. There was less consensus on down-regulated miRNAs. Conclusions: miRNAs yield a promising diagnostic biomarker potential in endometrial cancer, especially miR-205, the miR-200 family and miR-135b,-182,-183 and-223. However, no sufficient high quality data are available to draw hard conclusions. More research is needed to validate the diagnostic potential of these miRNAs in larger studies. In addition, the potential of urine as a non-invasive biofluid should be investigated in more detail.

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Section: Discussionmentioning

confidence: 99%

Diagnostic value of microRNA panel in endometrial cancer: A systematic review

2020

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“…In particular, the need for a viable alternative to PSA testing for PCa screening has generated a great interest in urine as a source of biomarkers in recent years. Compared to blood, the urinary circulating transcriptome has been little studied, and the vast majority of studies have been carried out on miRNAs and, specifically, on their presence in urine extracellular vesicles (uEVs) [15,[17][18][19][20][21][22]. Even though uEVs could represent an enriched source of biomarkers for PCa diagnosis, the need for highly specialized purification instrumentation such as ultracentrifuges makes such technique beyond the reach of the vast majority of routine clinical diagnostic laboratories.…”

Section: Discussionmentioning

confidence: 99%

The Urinary Transcriptome as a Source of Biomarkers for Prostate Cancer

Solé

Goicoechea

Goñi

et al. 2020

Cancers

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Prostate cancer (PCa) is the second most common cancer of men and is typically slow-growing and asymptomatic. The use of blood PSA as a screening method has greatly improved PCa diagnosis, but high levels of false positives has raised much interest in alternative biomarkers. We used next-generation sequencing (NGS) to elucidate the urinary transcriptome of whole urine collected from high-stage and low-stage PCa patients as well as from patients with the confounding diagnosis of benign hyperplasia (BPH). We identified and validated five differentially expressed protein-coding genes (FTH1 BRPF1, OSBP, PHC3, and UACA) in an independent validation cohort of small-volume (1 mL) centrifuged urine (n = 94) and non-centrifuged urine (n = 84) by droplet digital (dd)PCR. These biomarkers were able to discriminate between BPH and PCa patients and healthy controls using either centrifuged or non-centrifuged whole urine samples, suggesting that the urinary transcriptome is a valuable source of non-invasive biomarkers for PCa that warrants further investigation.

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“…Some groups centrifuge the urine samples before storage at high speed, while some groups use low speed to remove cell debris. Although some studies state that urine fraction does not affect the outcome, use of single or multiple biomarkers does; the lack of homogeneity in terms of urine fraction is also worth considering [79].…”

Section: Discussionmentioning

confidence: 99%

New Insights in Bladder Cancer Diagnosis: Urinary miRNAs and Proteins

et al. 2018

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Bladder cancer is the 10th-most common cancer worldwide. The diagnosis and follow-up of patients require costly invasive methods and due to these expenses, bladder cancer continues to be one of the expensive malignancies. Early diagnosis is crucial in bladder cancer as it is in other cancers; therefore, non-invasive biomarkers for early diagnosis are very important. In this review, we aimed to focus on the most recent investigations on potential urinary micro RNA (miRNA) and protein biomarkers for bladder cancer diagnosis and their associated pathways. Studies performed by different groups were compiled and the biomarker properties of various proteins and miRNAs in the urine of bladder cancer patients were evaluated. Key studies were obtained by searching keywords “bladder cancer, urinary miRNA, urinary protein, urinary biomarker”. Targets and the pathways of the miRNAs and proteins were analyzed according to mirBase Catalogue and Panther Database. The major pathways that are targeted by aberrantly expressed miRNAs are Cholecystokinin receptor (CCKR), p53, Wnt signaling pathway, and feedback loops. We hereby conclude that urinary micro RNAs and proteins are promising candidates for bladder cancer diagnosis. It should be noted that urine collection, storage conditions, choice of fraction, and normalization strategies should be standardized.

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Urine miRNA as a potential biomarker for bladder cancer detection – a meta-analysis

Cited by 20 publications

References 42 publications

Diagnostic value of microRNA panel in endometrial cancer: A systematic review

Diagnostic value of microRNA panel in endometrial cancer: A systematic review

The Urinary Transcriptome as a Source of Biomarkers for Prostate Cancer

New Insights in Bladder Cancer Diagnosis: Urinary miRNAs and Proteins

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