Urine sediment examination: A comparison of automated urinalysis systems and manual microscopy

Background:Urinary tract infection is a common condition which needs laboratory evaluation of urine to substantiate the clinical diagnosis and initiate treatment. The conventional urinalysis consists of using a test strip for chemical examination to identify the various urine sediments after which visual microscopy is done. We evaluate the analytical performance of automated microscopic technique (UF 500i) and compare results with those from manual microscopy. Materials and Methods:A total of 382 urine specimens were collected during a period of one month out of which 128 samples which had abnormal cell counts were analyzed for cells and particles by manual and automated microscopy by UF-500i flow cytometer.

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“…13 A good correlation to the above parameters were observed in our study. However, some limitations were considered in this study.…”

Section: Discussionsupporting

confidence: 86%

Automated urinalysis: First experiences and comparison of automated urinalysis system and manual microscopy

Gautam

Pyakurel

2014

J Pathol Nep

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“…Since intraand inter-observer reliability is a potential limitation, nephrology training programs should make a concerted effort to guarantee competency in the preparation and interpretation of urine microscopy [14]. This is even more critical going forward as many laboratories are employing automated technology to examine the urine [40,41], since the reliability of the automated systems for detection of casts is poor. Furthermore, nephrologists are superior in interpreting the urine sediment findings compared to laboratory personnel [42].…”

Section: Discussionmentioning

confidence: 98%

Acute tubular necrosis and pre-renal acute kidney injury: utility of urine microscopy in their evaluation- a systematic review

2009

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“…Urine microscopy fulfills all of these criteria. Furthermore, there is a trend toward automated microscopy at several hospitals in the United States (25,26). Although general clinicians depend on these automated systems, the performance of automated microscopy has not been thoroughly studied.…”

Section: Discussionmentioning

confidence: 99%

Urine Microscopy Is Associated with Severity and Worsening of Acute Kidney Injury in Hospitalized Patients

Perazella

Coca

Hall

et al. 2010

Clinical Journal of the American Society of Nephrology

116

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Background and objectives: Serum creatinine concentration at the time of nephrology consultation is not necessarily indicative of the severity of acute kidney injury (AKI). Although urine microscopy is useful to differentiate AKI, its role in predicting adverse clinical outcomes has not been well described.Design, setting, participants, & measurements: The relationship between urine microscopy findings at the time of nephrology consultation for AKI and clinical outcomes was evaluated prospectively. A urinary sediment scoring system was created on the basis of the number of renal tubular epithelial cells and granular casts. The primary outcome was worsening of AKI (progressing to higher AKI Network stage, dialysis, or death) during hospitalization.Results: Of 249 patients consulted for AKI, 197 had acute tubular necrosis or prerenal AKI and were included in the analysis. At consultation, 80 (40%) had stage 1, 53 (27%) had stage 2, and 66 (33%) had stage 3 AKI. The urinary sediment combined scores were lowest in those with stage 1 and highest in stage 3 AKI. Seventy-nine patients (40%) experienced worsening of AKI from the time of consultation. The urinary scoring system was significantly associated with increased risk of worsening AKI (adjusted relative risk: 7.3; 95% confidence interval: 4.5 to 9.7 for worsening with score of >3 versus score of 0) and was more predictive than AKI Network stage at the time of consultation.Conclusions: The urinary sediment score may be a useful tool to predict worsening of AKI due to either acute tubular necrosis or prerenal AKI during hospitalization.

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Urine sediment examination: A comparison of automated urinalysis systems and manual microscopy

Cited by 98 publications

References 21 publications

Automated urinalysis: First experiences and comparison of automated urinalysis system and manual microscopy

Automated urinalysis: First experiences and comparison of automated urinalysis system and manual microscopy

Acute tubular necrosis and pre-renal acute kidney injury: utility of urine microscopy in their evaluation- a systematic review

Urine Microscopy Is Associated with Severity and Worsening of Acute Kidney Injury in Hospitalized Patients

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