Urological Tumours

Sobin, L. H.; Gospodarowicz, M.K.; Wittekind, Ch.

doi:10.1002/9780471420194.tnmc38

Cited by 409 publications

(497 citation statements)

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“…Patients’ characteristics are given in Table . Histological evaluation was done according to the World Health Organization classification of tumors, and staging followed tumor lymph node metastasis (TNM) classification criteria . The primary endpoints of this study were overall survival (OS) and disease‐specific survival (DSS).…”

Section: Methodsmentioning

confidence: 99%

Circulating and tissue IMP3 levels are correlated with poor survival in renal cell carcinoma

Tschirdewahn

Panic

Püllen

et al. 2019

Intl Journal of Cancer

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Tissue protein expression of IMP3 is emerging as a promising prognostic factor in renal cell carcinoma (RCC). The most commonly used immunohistochemical (IHC) antibody has been criticized for its low specificity. In addition, blood levels of IMP3 have not yet been analyzed in RCC. Therefore, we compared the prognostic performance of two different IMP3 IHC antibodies and assessed the prognostic relevance of IMP3 plasma levels in RCC. IMP3 levels were assessed in an overall number of 425 RCC (344× clear cell [ccRCC], 63× papillary [pRCC], 18× chromophobe [chRCC]) patients in three partly overlapping cohorts. Plasma IMP3 concentrations were determined by ELISA in 98 RCC (79× ccRCC, 15× pRCC, 4× chRCC) patients and 20 controls. IMP3 mRNA expression levels were analyzed in 73 frozen tissue samples (55× ccRCC, 12× pRCC, 6× chRCC), while protein expressions were assessed in 366 FFPE samples (294× ccRCC, 56× pRCC, 16× chRCC) using the M3626 and N‐19 antibodies. IMP3 plasma and mRNA expression levels were significantly higher in patients compared to controls and in high‐grade compared to low‐grade tumors. In addition, IMP3 plasma and tissue protein levels (by M3626) were higher and IMP3 mRNA expression levels tended to be higher in patients with distant metastasis. Multivariate analyses in clear cell RCC revealed high IMP3 plasma concentration and mRNA expression as independent predictors of disease‐specific survival. IMP3 immunostainings by M3626 but not by N‐19 were independently associated with poor overall and disease‐specific survival. High plasma and tissue levels of IMP3 are independently associated with poor RCC prognosis. The applied antibody significantly impacts the prognostic performance of analysis. IMP3 analysis may improve risk‐stratification of RCC patients and therefore could help to optimize therapeutic and follow‐up decisions.

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Section: Methodsmentioning

confidence: 99%

Circulating and tissue IMP3 levels are correlated with poor survival in renal cell carcinoma

Tschirdewahn

Panic

Püllen

et al. 2019

Intl Journal of Cancer

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“…Clinical features included age and sex. All pathologic features were evaluated by 1 expert uropathologist (A.H.) and included subtype according to the World Health Organization classification of tumors12; tumor‐lymph node‐metastasis (TNM) classification13; Fuhrman grade14; the presence of microscopic coagulative tumor necrosis; and pathologic tumor size according to the greatest tumor dimension. After surgery, patients with N0M0 disease were followed according to a risk‐stratified surveillance protocol15 with intermittent imaging of the chest, abdomen, and pelvis.…”

Section: Methodsmentioning

confidence: 99%

Serum cell‐free DNA in renal cell carcinoma

Martino

Klatte

Haitel

et al. 2011

Cancer

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BACKGROUND: Currently, there are no established diagnostic and prognostic serum markers for renal cell carcinoma (RCC). The objective of this study was to evaluate the putative significance of serum cell-free DNA. METHODS: Preoperative serum samples from 200 consecutive patients with sporadic, solid renal tumors were analyzed (157 patients with RCC and 43 patients with benign renal tumors). Quantitative real-time polymerase chain reaction was used to assess total cell-free DNA (ring finger protein 185 [RNF185]) and CpG island methylation of Ras association domain family member 1A (RASSF1A) von Hippel-Lindau (VHL), prostaglandin-endoperoxidase synthase 2 (PTGS2), and P16 (cyclin-dependent kinase inhibitor 2A). Associations with RCC, pathologic variables, and disease-specific survival were evaluated. RESULTS: Total cell-free DNA levels and CpG island methylation of RASSF1A and VHL were highly diagnostic for RCC with an area under the receiver operating characteristic curve of 0.755, 0.705, and 0.694, respectively. VHL methylation was detected more frequently in patients with clear cell RCC than in those with other subtypes (P ¼ .007). Total cell-free DNA levels were higher in patients with metastatic RCC (P < .001) and necrotic RCC (P ¼ .003) and were associated with poorer disease-specific survival (P < .001). In multivariate analysis, the tumor stage, size, grade, and necrosis (SSIGN) score (P < .001) and categorized total cell-free DNA levels (P ¼ .028) were retained as independent prognostic factors. CONCLUSIONS: The current results indicated that cell-free DNA represents a novel serum-based diagnostic and prognostic biomarker for RCC. Total serum cell-free DNA levels and CpG island methylation of RASSF1A and VHL may be useful diagnostic biomarkers for RCC. VHL methylation of cellfree DNA is suggestive of clear cell RCC. Total serum cell-free DNA may be a useful prognostic biomarker that may assist in tailoring postoperative surveillance and therapy. External prospective validation of these data will be required. Cancer 2012;118:82-

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“…The database included patient age, sex, Eastern Cooperative Group performance status,11 Fuhrman grade,12 tumor size, CSS, and RFS. All investigated tumors were pathologically confirmed ccRCCs according to the 2009 TNM classification 13. Computed tomography or magnetic resonance imaging studies of the chest, abdomen, and pelvis as well as x‐rays in a subset of patients were obtained to assess the extent of disease before surgery.…”

Section: Methodsmentioning

confidence: 99%

Deletions of chromosomes 3p and 14q molecularly subclassify clear cell renal cell carcinoma

Kroeger

Klatte

Chamie

et al. 2013

Cancer

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BACKGROUND: The short arm of chromosome 3 (3p) harbors the von Hippel‐Lindau (VHL) tumor suppressor gene, and the long arm of chromosome 14 (14q) harbors the hypoxia‐inducible factor 1α (HIF‐1α) gene. The objective of this study was to evaluate the significance of 3p loss (loss VHL gene) and 14q loss (loss HIF‐1α gene) in clear cell renal cell carcinoma (ccRCC). METHODS: In total, 288 ccRCC tumors underwent a prospective cytogenetic analysis for alterations in chromosomes 3p and 14q. Tumors were assigned to 1 of 4 possible chromosomal alterations: VHL +3p/+14q (VHL wild type [VHL‐WT]), VHL +3p/−14q (VHL‐WT plus HIF2α [WT/H2]), −3p/+14q (HIF1α and HIF2α [H1H2]), and −3p/−14q (HIF2α [H2]). RESULTS: Among patients who had loss of 3p, tumors with −3p/−14q (H2) alterations were larger (P = .002), had higher grade (P = .002) and stage (P = .001), and more often were metastatic (P = .029) than tumors that retained 14q (H1H2). All patients who had tumors with −3p/−14q (H2) had worse cancer‐specific survival (P = .014), and patients who had localized disease (P = .012) and primary T1 (pT1) tumors (P = .008) had worse recurrence‐free survival. In patients who had pT1 tumors, combined 3p/14q loss was an independent predictor of recurrence‐free survival (hazard ratio, 11.19; 95% confidence interval, 1.91‐65.63) and cancer‐specific survival (hazard ratio, 15.93; 95% confidence interval, 3.09‐82.16). The current investigation was limited by its retrospective design, single‐center experience, and a lack of confirmatory protein analyses. CONCLUSIONS: Loss of chromosome 3p (the VHL gene) was associated with improved survival in patients with ccRCC, whereas loss of chromosome 14q (the HIF‐1α gene) was associated with worse outcomes. The results of the current study support the hypothesis that HIF‐1α functions as an important tumor suppressor gene in ccRCC. Cancer 2013. © 2013 American Cancer Society.

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Urological Tumours

Cited by 409 publications

References 0 publications

Circulating and tissue IMP3 levels are correlated with poor survival in renal cell carcinoma

Circulating and tissue IMP3 levels are correlated with poor survival in renal cell carcinoma

Serum cell‐free DNA in renal cell carcinoma

Deletions of chromosomes 3p and 14q molecularly subclassify clear cell renal cell carcinoma

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