Uropathogenic Escherichia coli virulence and innate immune responses during urinary tract infection

Ulett, Glen C.; Totsika, Makrina; Schaale, Kolja; Carey, Alison J.; Sweet, Matthew J.; Schembri, Mark A.

doi:10.1016/j.mib.2013.01.005

Cited by 176 publications

(153 citation statements)

References 80 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…Thus, the bitter/umami polymodality of chemosensory cells may serve to broaden the spectrum for recognition of potential hazardous material in the urethral lumen. Most importantly, these chemosensory cells responded to heat-inactivated uropathogenic E. coli, the primary cause of urinary tract infection (43).…”

Section: Discussionmentioning

confidence: 99%

Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes

Deckmann

Filipski

Krasteva‐Christ

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

148

214

View full text Add to dashboard Cite

Significance We report the presence of a previously unidentified cholinergic, polymodal chemosensory cell in the mammalian urethra, the potential portal of entry for bacteria and harmful substances into the urogenital system. These cells exhibit structural markers of respiratory chemosensory cells (“brush cells”). They use the classical taste transduction cascade to detect potential hazardous compounds (bitter, umami, uropathogenic bacteria) and release acetylcholine in response. They lie next to sensory nerve fibers that carry acetylcholine receptors, and placing a bitter compound in the urethra enhances activity of the bladder detrusor muscle. Thus, monitoring of urethral content is linked to bladder control via a previously unrecognized cell type.

show abstract

Section: Discussionmentioning

confidence: 99%

Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes

Deckmann

Filipski

Krasteva‐Christ

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

148

214

View full text Add to dashboard Cite

show abstract

“…These cells then enter the bloodstream and mature. The mature neutrophils then respond to signals from G-CSF (and other molecules) and travel to the site of infection; in this instance, the bladder [51,52].…”

Section: Cytokines and Other Effectorsmentioning

confidence: 99%

Innate Immune Response to Urinary Tract Infections Involving Escherichia coli

Reygaert¹

2014

J Clin Cell Immunol

View full text Add to dashboard Cite

The innate immune system responds in a rapid, initially nonspecific manner to infection in the urinary tract. There are many molecules and cells involved in this response. These include: antimicrobial peptides, toll-like receptors, chemokines, cytokines, and neutrophils. The most common cause of urinary tract infections (UTIs) are uropathogenic Escherichia coli (UPEC). The innate immune system responds to the presence of flagella, fimbriae, and the lipopolysaccharide outer membrane of these bacteria. Antimicrobial peptides are used to lyse the bacteria and also prevent the bacteria from binding to epithelial cells in the urinary tract. The toll-like receptors sense the presence of the bacteria and signal for the production of molecules that cause immune and inflammation responses. The various chemokines and cytokines such as CXCL8, CCL2, interleukins (IL-6, IL-8, IL-10, IL-17A), and granulocyte colony stimulating factor (G-CSF), are used for much of the signaling in innate immunity. In addition, neutrophils play a major role in rapid removal of invading bacteria. The rapid innate immune response is designed to remove most of the bacteria within 24 hours in an uncomplicated UTI. This review presents an overview of the innate immune response to UTIs caused by UPEC and the host molecules and cells involved.

show abstract

“…UTIs usually start as bladder infections (cystitis), but can develop into acute kidney infections (pyelonephritis), ultimately resulting in scarring and renal failure. UTIs are caused by a range of pathogens, with uropathogenic Escherichia coli (UPEC) being the most common UPEC (more than 80% of all UTIs) etiological agent [3]. UPEC strains possess harmful factors including fimbrial adhesins, toxins, flagella, auto transporter proteins and iron-acquisition systems, and that contribute to cause clinical diseases [4].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Lactic Acid Bacteria on Uropathogenic Escherichia coli-Induced Urinary Tract Infections

Liu¹,

Ho²

2016

J Prob Health

View full text Add to dashboard Cite

The aim of this study evaluated the in vitro and in vivo antimicrobial activity of selected lactic acid bacteria (LAB) against UPEC for prevention and amelioration of UTIs. We screened LAB strains with antimicrobial effects on UPEC using a well-diffusion assay, bacterial adherence to the uroepithelium cell line SV-HUC-1 (BCRC 60358), and a coculture inhibition assay. The results showed that the 7 LAB strains (Lactobacillus paracasei, L. salivarius, two Pediococcus pentosaceus strains, two L. plantarum strains, and L. crispatus) and the fermented probiotic products produced by these multi-LAB strains exhibited potent zones of inhibition against UPEC. Moreover, the LAB strains and probiotic products adhered strongly to the uroepithelium SV-HUC-1 cell line. The growth of UPEC strains was also markedly inhibited after co-culture with the LAB strains and probiotic products in human urine. In addition, the enhanced levels of IL-6, IL-8 and lactic acid dehydrogenase were significantly decreased by treatments with the LAB strains and probiotic products in UPEC-induced SV-HUC-1 cells. Furthermore, oral administration of probiotic products reduced the number of viable UPEC in the urine of UPEC-challenged BALB/c mice. Taken together, this study demonstrates that probiotic supplementation may be useful as an adjuvant therapy for the treatment of bacterial-induced urinary tract infections.

show abstract

Uropathogenic Escherichia coli virulence and innate immune responses during urinary tract infection

Cited by 176 publications

References 80 publications

Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes

Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes

Innate Immune Response to Urinary Tract Infections Involving Escherichia coli

Inhibitory Effect of Lactic Acid Bacteria on Uropathogenic Escherichia coli-Induced Urinary Tract Infections

Contact Info

Product

Resources

About