Urtica dioica L. ve Myrtus communis L. Yapraklarının Sulu ve Etanolik Ekstraktlarının Sığır Sindirim Strongilozu Üzerine Antelmintik Aktivitesi: İn-vitro Çalışma

Abstract

The present research aimed to evaluate the in-vitro anthelmintic activity of M. communis and U. dioica against digestive strongyles in naturally infected cattle. The anthelmintic activities of the extracts were evaluated using the egg hatch assay and larval mortality assay. Leaves powder of M. communis and U. dioica was extracted by maceration. Ethanolic and water extracts were test at 0.78, 1.55, 3.1, 6.2, 12.5, 25 and 50 mg/ml. Albendazole and DMSO was used as a positive and negative control at concentration 20 mg/ml and 3%, respectively. The results of regression line indicated that LC50 of Urtica dioica EE and EA were very low namely 2.57, 3.02 and 3.22 mg/ml; and 4.90, 4.67 and 3.24 mg/ml for inhibition of embryonation eggs, hatching rate and L1 mortality, respectively. The mean embryonation rate maximum was 100% at 25 and 50 mg/ml concentration in AE and EE U. dioica; whereas M. communis recorded a maximum level of 57.1 ± 3.12% and 63.95 ± 3.01% at 50 mg/ml concentration for the AE and EE, respectively. The extracts of U. dioica leaves high effects (100%) were observed with 25 and 50 mg/ml in both extracts, whereas M. communis recorded a high level at 50mg/ml concentration for the AE and EE (58.05±3.69% and 65.13±3.13%, respectively). The larval mortality rate of both AE and EE from U. dioica showed that the extracts at and 25 and 50 mg/ml exhibited 100 % at 24h of contact. In conclusion, these findings showed that, aqueous and ethanolic extracts of U. dioica and M. communis leaves have a potential anthelmintic activity on eggs and larvae of bovine strongly parasites.