Usage of 3-methyl-1-β-cyclodextrinimidazole tosylate for electrophoretic separation and preconcentration of corticosteroids by capillary electrophoresis

Кравченко, А. В.; Колобова, Е. А.; Карцова, Л. А.

doi:10.1007/s00706-021-02809-4

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…The method to analyze steroids has been usually developed with HPLC and CG, 37,38 whereas there are plenty of approaches to determine steroid hormone by CE that have been recently developed 39–43 . This work demonstrated the possibility of using the tr‐trapping technique for sample preparation with the MS instrument without the interference of the micelle molecule.…”

Section: Resultsmentioning

confidence: 99%

“…The method to analyze steroids has been usually developed with HPLC and CG, 37,38 whereas there are plenty of approaches to determine steroid hormone by CE that have been recently developed. [39][40][41][42][43] This work demonstrated the possibility of using the tr-trapping The tr-trapping-ESI-MS procedure is simple since it can be automatically programmed the injection and separation in one instrument. Although the developed method required 50 min to operate the separation, this method can guarantee that SDS would not cause the problem with the MS instrument because it requires around twofold of time to migrate through the capillary channel.…”

Section: Detection Of Steroids With Trtrapping-esi-msmentioning

confidence: 89%

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Development of transient trapping micellar electrokinetic chromatography coupled with mass spectrometry for steroids analysis

et al. 2022

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An on‐line sample preconcentration technique based on transient trapping (tr‐trapping) in micellar electrokinetic chromatography (MEKC) was applied for steroid detection with UV (tr‐trapping‐UV) and electrospray ionization mass spectrometry detection (tr‐trapping‐ESI‐MS). ESI‐MS was used to improve the sensitivity in MEKC. The MEKC separation was carried out using volatile ammonium formate as a background solution to facilitate the coupling with ESI‐MS. The partial introduction of a sodium dodecyl sulfate (SDS) micellar solution before the introduction of a sample solution to the capillary provided the effective preconcentration of analytes. At the same time, the SDS micelle would not enter the ESI‐MS system, so its interference in ESI‐MS detection was suppressed under the optimal condition, then five steroids can be separated by the developed method. In tr‐trapping‐ESI‐MS, an acidic condition of pH 3.5 was employed to suppress the electroosmotic flow, which can avoid micellar solution migrating to the MS instrument. The developed method showed that the micellar solution requires a twofold slower time than the sample to migrate along the column, which can prohibit the cause of the problem with the MS instrument and interference signal of SDS in the steroid's detection. The tr‐trapping‐ESI‐MS protocol showed up to 540‐fold enhancements of the peak intensity and 50‐fold improvement of the limit of detection compared with capillary zone electrophoresis using androsterone as a model sample.

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“…The method to analyze steroids has been usually developed with HPLC and CG, 37,38 whereas there are plenty of approaches to determine steroid hormone by CE that have been recently developed 39–43 . This work demonstrated the possibility of using the tr‐trapping technique for sample preparation with the MS instrument without the interference of the micelle molecule.…”

Section: Resultsmentioning

confidence: 99%

“…The method to analyze steroids has been usually developed with HPLC and CG, 37,38 whereas there are plenty of approaches to determine steroid hormone by CE that have been recently developed. [39][40][41][42][43] This work demonstrated the possibility of using the tr-trapping The tr-trapping-ESI-MS procedure is simple since it can be automatically programmed the injection and separation in one instrument. Although the developed method required 50 min to operate the separation, this method can guarantee that SDS would not cause the problem with the MS instrument because it requires around twofold of time to migrate through the capillary channel.…”

Section: Detection Of Steroids With Trtrapping-esi-msmentioning

confidence: 89%

Development of transient trapping micellar electrokinetic chromatography coupled with mass spectrometry for steroids analysis

et al. 2022

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“…In the field-amplified stacking injection (FASI) technique, more efficient sample enrichments can be generally attained by electrokinetically injecting a low-conductivity sample solution. From the year 2019 to 2021, FASI has been applied to the analyses of macrocyclic antibiotics [22], diltiazem and metoprolol tartrate [23], cold medicine compounds [24], corticosteroids [25], α-blockers [26], 2,2'-azobis(2-amidinopropane) ion [27], sulfonamides [28], chlorophenols [29], cathinones [30], 9-hydroxyrisperidone [31], linear alkylbenzene sulfonates [32], quetiapine and its metabolites [33] as summarized in Table 2. Kowalski et al reported an FASI method for stacking macrocyclic antibiotics in hydrophobic interaction electrokinetic chromatography [22].…”

Section: Application Of Field-amplified Sample Stackingmentioning

confidence: 99%

Recent Applications of Dynamic On-Line Sample Preconcentration Techniques in Capillary Electrophoresis

Kitagawa

SOMA

2022

Chromatography

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This review highlights recent applications of on-line sample preconcentration techniques in capillary electrophoresis (CE) from 2019 to 2021. Various preconcentration techniques based on the analyte velocity change in two or three discontinuous solutions system including field-amplified sample stacking, transient isotachophoresis, pH-mediated stacking, sweeping, and their modified and combined techniques have been employed to enrich and separate biological, environmental, food samples, and so on, in CE. More than 60 published research articles collected from Scopus databases described the dynamic on-line sample preconcentration techniques. This review provides comprehensive tables listing the applications of the on-line sample preconcentration techniques.

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“…All these facts point to the possible successful application of alkyl‐imidazolium‐β‐CD tosylate for the separation of acidic racemate of ketorolac. In addition, previously we had experience with the application of this type of derivative for hydrophobic corticosteroids [38].…”

Section: Introductionmentioning

confidence: 99%

Development of a capillary electrophoretic method for determination of ketorolac enantiomers in human plasma using cationic β‐cyclodextrin derivative as a chiral selector

Кравченко

Колобова

Kechin

et al. 2022

J of Separation Science

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A novel approach for the separation of ketorolac enantiomers by capillary electrophoresis is presented. A cationic β‐cyclodextrin derivative based on imidazole was synthesized and used as a chiral selector in the background electrolyte. The influence of pH and ionic strength of background electrolyte, as well as cationic β‐cyclodextrin derivative concentration on the resolution of ketorolac enantiomers, was investigated. The highest value of the resolution for ketorolac enantiomers was 1.46 when the background electrolyte consisted of 25 mM NaH2PO4 (pH 6.4) with 1 mM 1‐butyl‐3‐β‐cyclodextrinimidazolium tosylate. Additionally, the possibilities of cationic derivatives for the separation of ketoprofen enantiomers were shown (peak resolution 1.06). The two‐step preconcentration mode was developed to reduce the limit of detection of individual enantiomers. The proposed approach was successfully applied to determine ketorolac enantiomers in tablet “Ketorol express” and human plasma. The calibration range of ketorolac enantiomers for plasma samples was 0.25–2.50 μg/ml with coefficients of determination ≥ 0.99. The relative standard deviation both of the peak area and migration time was less than 15%, as well as the accuracy ranged from 90.1% to 110.2% for both analytes. The limits of detection were 44 and 55 ng/ml for R‐ and S‐ketorolac. The quantity of ketorolac in plasma was verified with high‐performance liquid chromatography.

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Usage of 3-methyl-1-β-cyclodextrinimidazole tosylate for electrophoretic separation and preconcentration of corticosteroids by capillary electrophoresis

Cited by 5 publications

References 17 publications

Development of transient trapping micellar electrokinetic chromatography coupled with mass spectrometry for steroids analysis

Development of transient trapping micellar electrokinetic chromatography coupled with mass spectrometry for steroids analysis

Recent Applications of Dynamic On-Line Sample Preconcentration Techniques in Capillary Electrophoresis

Development of a capillary electrophoretic method for determination of ketorolac enantiomers in human plasma using cationic β‐cyclodextrin derivative as a chiral selector

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