Usage of GD-95 and GD-66 lipases as fusion partners leading to improved chimeric enzyme LipGD95-GD66

Malunavicius, Vilius; Druteika, Gytis; Sadauskas, Mikas; Veteikytė, Aušra; Matijošytė, Inga; Lastauskienė, Eglė; Gegeckas, Audrius; Gudiukaitė, Renata

doi:10.1016/j.ijbiomac.2018.07.002

Cited by 11 publications

(4 citation statements)

References 45 publications

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“…1B, lower panel). Although the structure of this lipase has not been determined, this result suggests that the N-terminus of this enzyme plays an important role in catalytic activity, in accordance with previous results showing that lipase activity can be disrupted when fused to N-terminal fusion partners [19][20][21].…”

Section: Resultssupporting

confidence: 89%

Bio-specific immobilization of enzymes on electrospun PHB nanofibers

Jang

Park

Lim

et al. 2021

Enzyme and Microbial Technology

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Section: Resultssupporting

confidence: 89%

Bio-specific immobilization of enzymes on electrospun PHB nanofibers

Jang

Park

Lim

et al. 2021

Enzyme and Microbial Technology

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“…Moreover, the Geobacillus sp. 95 strain has already been successfully explored as a source of highly active, thermostable lipolytic enzymes [ 19 ]. Thus, this study highlights the attractiveness of the Geobacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…The strain Geobacillus sp. 95 was deposited in the DSMZ culture collection under the number DSM 104629 [ 19 ]. For the primary screening, Geobacillus spp.…”

Section: Methodsmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles Produced Using Geobacillus spp. Bacteria

et al. 2023

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Silver nanoparticles (AgNPs) are well known for their unique physical and chemical properties, which can be incorporated into a wide range of applications. The growing resistance of microorganisms to antimicrobial compounds promoted the use of AgNPs in antimicrobial therapy. AgNPs can be obtained using physical and chemical methods, but these technologies are highly unfriendly to nature and produce large amounts of side compounds (for example, sodium borohydride and N,N-dimethylformamide). Therefore, alternative technologies are required for obtaining AgNPs. This report focuses on the biosynthesis of silver nanoparticles through the reduction of Ag+ with the cell-free secretomes of four Geobacillus bacterial strains, namely, 18, 25, 95, and 612. Only a few studies that involved Geobacillus bacteria in the synthesis of metal nanoparticles, including AgNPs, have been reported to date. The silver nanoparticles synthesized through bio-based methods were characterized using UV–Vis spectroscopy, scanning electron microscopy (SEM), dynamic light scattering (DLS), and zeta potential measurements. UV–Vis spectroscopy showed a characteristic absorbance peak at 410–425 nm, indicative of AgNPs. SEM analysis confirmed that most nanoparticles were spherical. DLS analysis showed that the sizes of the obtained AgNPs were widely distributed, with the majority less than 100 nm in diameter, while the zeta potential values ranged from −25.7 to −31.3 mV and depended on the Geobacillus spp. strain.

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“…After the examination, the activity of seven isolates that had the ability to produce lipase enzyme was measured after culturing them in (MSM) to choose the isolate with the highest activity as shown in Table (1). This method is consistent with Malunavicius where a liquid medium was used and incubated at 37 °C for 24 hours in a shaker incubator at 150 rpm [20]. 20.9…”

Section: Lipase Assaymentioning

confidence: 99%

Isolation Of Pseudomonas Aeruginosa from Soil and Production of Lipase Enzyme

Ali

Hameed

Nadder

2022

IOP Conf. Ser.: Earth Environ. Sci.

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The isolates of Pseudomonas aeruginosa bacteria were uncovered in the soil surrounding the roots of palms and public gardens in Baghdad for the production of lipase enzyme. The lipase enzyme has many applications that are included in the textile and food industry, and the manufacture of detergents and medical preparations. Several tests such as temperature change, incubation period, change of lipid sources, nitrogen sources such as peptone and tryptone, and carbon sources such as glucose and lactose were carried out to choose suitable conditions for bacterial growth. The results indicated studying the conditions affecting production, it was noted that the best production was when using the culture medium to which 1% of corn oil was added, pH 7, at a temperature of 37 °C and an incubation period of 24 hours in vibrating incubator at 151 rpm, The soil surrounding the roots of the plant is a good reservoir for the presence of Pseudomonas aeruginosa bacteria

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Usage of GD-95 and GD-66 lipases as fusion partners leading to improved chimeric enzyme LipGD95-GD66

Cited by 11 publications

References 45 publications

Bio-specific immobilization of enzymes on electrospun PHB nanofibers

Bio-specific immobilization of enzymes on electrospun PHB nanofibers

Biosynthesis of Silver Nanoparticles Produced Using Geobacillus spp. Bacteria

Isolation Of Pseudomonas Aeruginosa from Soil and Production of Lipase Enzyme

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