Use and Interpretation of Diagnostic Immunologic Laboratory Tests

deShazo, Richard D.; Lopez, M.; Salvaggio, John E.

doi:10.1001/jama.1987.03400200217029

Cited by 15 publications

(1 citation statement)

References 22 publications

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“…The consensus of the group was that they could sustain a 25% increment of training volume throughout the 3 weeks. In fact, the activity questionnaires showed that the weekly distance had been increased 4564 (996) 38 (24) by 35%, and since most subjects also had some increase of speed over the same route, the estimated training volume was increased by an average of 38% On average, the heavy training did not lead to any gains of maximal aerobic power (Table 2). In all except three subjects, the difference of maximal oxygen intake between the two visits was less than 5%.…”

Section: Modification Of Training Planmentioning

confidence: 99%

Potential markers of heavy training in highly trained distance runners.

Verde

Thomas²,

Shephard³

1992

Br J Sports Med

127

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Markers of a heavy increase in training were examined in ten highly trained distance runners (mean(s.d.) age 29.8(1.7) years, maximal oxygen intake 65.3 ml kg-minu-, personal best 10-km time 31 min 4s) who undertook a deliberate 38% increment of training over a 3-week period. Their running performance did not improve, and six of the ten subjects developed sustained fatigue, suggesting that training was excessive, although the full clinical picture of overtraining did not develop. The Profile of Mood States was the best single marker of disturbed function, indicating increased fatigue and decreased vigour. There were no useful changes of resting heart rate or perceived exertion during submaximal running, sleep was undisturbed, and there were no orthopaedic injuries. Two subjects developed rhinoviral infections following the heavy training, and a third complained of symptoms that were diagnosed 2 weeks later as exercise-induced asthma. The increase of serum cortisol normally induced by 30 min of submaximal exercise was no longer seen when the same acute exercise was performed after heavy training. Resting lymphocyte proliferation tended to increase in response to phytohaematoglutinin (PHA) and concanavalin A (Con A), the ratio of helper to suppressor cells (H/S) decreased, and pokeweed mitogen induced smaller increases in IgG and IgM synthesis. Whereas before heavy training, PHAstimulated lymphocyte proliferation was unchanged by 30 min of acute submaximal exercise, after 3 weeks of heavy training the same bout of exercise caused an 18% suppression of proliferation. Likewise, heavy training brought about a decrease of T-lymphocytes in response to acute submaximal exercise, but an abolition of the acute exercise-induced decrease in the HIS ratio. The previously observed exercise-induced decrease of IgG synthesis did not occur when the same acute bout of exercise was performed after heavy training. We conclude that such minor and transient changes of immune function may possibly be a warning that training is becoming excessive, but they have only a limited significance for overall immune function.

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Section: Modification Of Training Planmentioning

confidence: 99%

Potential markers of heavy training in highly trained distance runners.

Verde

Thomas²,

Shephard³

1992

Br J Sports Med

127

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The Utility of Routine Screening of Patients With Uveitis for Systemic Lupus Erythematosus or Tuberculosis

Rosenbaum

Wernick

1990

Arch Ophthalmol

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Introduction to Diagnostic Laboratory Immunology

Fleisher¹

1997

JAMA

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Assays performed in the diagnostic immunology laboratory support the diagnosis and management of a wide spectrum of clinical conditions. This chapter reviews immunologic principles as they apply to diagnostic laboratory assays. Most of the determinations are based on well-established principles of antigen-antibody reactions. Some of the specific areas discussed include flow cytometric analyses, critical in the care of patients with hematologic malignancies, with the acquired immunodeficiency syndrome, or undergoing transplantation, and protein electrophoretic assays to identify the presence of monoclonal gammopathies. We also discuss the use of molecular techniques in the diagnosis of hematologic malignancies and primary immunodeficiencies, characterization of the major histocompatibility complex, and enumeration of viral burden.

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Use and Interpretation of Diagnostic Immunologic Laboratory Tests

Cited by 15 publications

References 22 publications

Potential markers of heavy training in highly trained distance runners.

Potential markers of heavy training in highly trained distance runners.

The Utility of Routine Screening of Patients With Uveitis for Systemic Lupus Erythematosus or Tuberculosis

Introduction to Diagnostic Laboratory Immunology

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