Use and perceptions of plastination among medical anatomy educators in the United States

Klaus, Rachel; Royer, Danielle; Stabio, Maureen E.

doi:10.1002/ca.23025

Cited by 16 publications

(17 citation statements)

References 35 publications

(56 reference statements)

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“…When used for educational purpose, the plastinates can be accessed at any time and allow the easy transportation of specimens to distant teaching sites outside the dissection hall without the need for carrying them in preservative laden containers (Rahul et al, 2020). Plastination likewise enables the students, academics and researchers to study an organ or tissue without being obliged by an actual division among himself and the specimen, that is, a container or gloves (Latorre et al, 2016;Klaus et al;Rahul et al). In the present study, we analyzed the histomorphology and biometry of different organs i.e.…”

Section: Discussionmentioning

confidence: 99%

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Histological Architectures and Biometric Characteristics of Indigenously Plastinated Organs of Goat

2021

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The histo-morphology and biometry of liver, lung and kidney of goat were studied. The obtained samples were divided into two groups. One set of tissue was processed for paraffin embedding after formalin fixation. The other set was indigenously plastinated. The plastinates were embedded with paraffin without deplastination. Both the non plastinated and plastinated tissues were sectioned and processed for routine staining. The tissues were examined under light microscope for histological architectures and quantitatively assessed the biometric parameters. The non plastinated and plastinated samples yielded mostly similar histological architectures. But plastinated liver showed alterations and artifacts with enlargement of the central vein and hepatic sinusoidal space. Plastinated lung revealed enlarged alveolar sac. Lack of nuclear clarity was observed for all the plastinated samples. The plastinated kidney revealed compactness of the cellular structures and shrinkage induced artifacts with clear renal corpuscles structure and obvious glomerular capsule (Bowman's capsule). The biometric measurements of central vein of liver, alveolar sac of lung, glomerulus and glomerular space of kidney of both plastinated and non plastinated tissue were also performed using calibrated stage micrometer. Comparison was done between the biometric data for both plastinated and non plastinated tissue. No significant difference was found in the obtained biometric data of liver and lung. Only a significant difference was observed between the width of glomerulus of non plastinated kidney and width of glomerulus of plastinated kidney. The morphological and biometric study of non plastinated and plastinated organs of goat can conclude that histological slides can be made from indigenously plastinated organs with well preserved histological architectures of the tissues with some rare exceptions. Plastination does not affect the biometric characteristics of the organs. In addition, the comparative morphologic and biometric study of plastinated and non plastinated organs of goat will be useful in education and research.

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Section: Discussionmentioning

confidence: 99%

“…According to the findings of Klaus et al (2017), plastinates at present don't permit further analyzation or histology based studies which forestalls its utilization for research purposes. Plastinated specimens embedded in paraffin cannot always be used for microscopy because of artifacts produced during cutting (Grondin et al).…”

Section: Introductionmentioning

confidence: 99%

Histological Architectures and Biometric Characteristics of Indigenously Plastinated Organs of Goat

2021

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“…It is done by converting the tissue into a durable plastic model by a process, which replaces the water in it with a polymer (mostly silicon). Even though the process is time consuming, it is beneficial, especially in preserving rare specimens since the plastic models or "plastinates" last forever without the need for preservative (von Hagens, 1986;Klaus et al, 2018). It is valued as a safe and inexpensive alternative to formalin as a long-term preservative and has been previously elucidated in the literature (Ravi and Bhat, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Plastinates at present do not allow further dissection or histologybased studies. This prevents its use for research purposes, especially in the case of rare specimens (Klaus et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Deplastination: Preservation of Histological Structures and its Anticipated Role in the Field of Histopathology

Rahul¹,

Francis

Pandit³

et al. 2019

Clinical Anatomy

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Deplastination is the process of reversing plastination such that a plastinated specimen can be reverted to its raw nature. This would enable its use in the field of histopathology. The present study aims to ascertain if deplastinates can be used for histopathological studies after a time period. Tissue samples were taken from patients undergoing maxillofacial surgeries for oral carcinomas after obtaining written informed consent. The 12 specimens obtained were divided into two groups. One set of tissues was processed for paraffin embedding after 10% formalin fixation. The other set was plastinated by S10 silicon plastination. After 3 months, the plastinates were deplastinated using sodium methoxide and processed for routine hematoxylin and eosin staining, similar to the formalin fixed specimens. The slides were quantitatively assessed on parameters like tissue architecture, staining property, and intracellular structure. In addition, the slides were qualitatively evaluated by a pathologist who was blinded to the mode of preservation to see if identification of pathological features was possible on a deplastinated slide. The formalin preserved specimens and deplastinated tissue slides compared closely in all three parameters tested with the need to identify the endpoint of deplastination. Qualitatively, deplastination did not hamper identification of tissue pathology. Deplastination increases the scope of a stored plastinate by allowing histological studies in the future without the need for any preservatives or special storage equipment. It preserves structure and maintains tissue pathology. An improved method of ensuring the endpoint of deplastination needs to be identified. Clin. Anat. 32:108–112, 2019. © 2019 Wiley Periodicals, Inc.

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“…Plastination specimens did not completely replace the traditional guided animal dissection; similar result was mentioned by Klaus et al (2018). Moreover, the combination of the plastinated specimens with the traditional wet specimens in the teaching and learning of veterinary gross anatomy can be beneficial depending on the topic being taught at the time; similar result was mentioned by Latorre et al (2016).…”

Section: Discussionmentioning

confidence: 75%

Production and use of plastinated anatomical specimens as teaching and learning tools in veterinary gross anatomy in the Caribbean

Mohamed¹,

John²

2018

J Adv Vet Anim Res

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Objective: Veterinary Anatomy is considered as the backbone subject in the veterinary medicine program. Formalized specimens were not very much accepted by the veterinary students due to their wetness, bad smell and potential harmful effects. Plastination has evolved as a new technique for prolonged preservation of specimens by replacing the water and fat by a curable plastic polymer. Material and methods:The process of plastination involved fixation, dehydration, impregnation and curing. The plastinated specimens were used for teaching and learning of veterinary gross anatomy courses for veterinary students. Results: Plastinated specimens were appreciated by the staff and students as they were odorless, non-toxic and durable, needed less space for storage and they were used in teaching the veterinary anatomy courses. Plastinated specimens were used for studying not only in the dissection hall but also in the lecture room, both individually and in groups. Conclusion: The plastinated specimens were excellent for demonstration but they could not completely replace the traditional dissection because students learn best by exploratory and hands-on dissection. Moreover, the wet specimens provided a more accurate visual representation of the body parts and skills to aid in dealing with actual clinical cases in the future. Therefore, the plastinated and wet specimens were used simultaneously depending on the topic being taught at the time.

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Use and perceptions of plastination among medical anatomy educators in the United States

Cited by 16 publications

References 35 publications

Histological Architectures and Biometric Characteristics of Indigenously Plastinated Organs of Goat

Histological Architectures and Biometric Characteristics of Indigenously Plastinated Organs of Goat

Deplastination: Preservation of Histological Structures and its Anticipated Role in the Field of Histopathology

Production and use of plastinated anatomical specimens as teaching and learning tools in veterinary gross anatomy in the Caribbean

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