Use of 2-Thiobarbituric Acid—Malonaldehyde Reaction as a Measure of Antioxidant Effectiveness in Pharmaceutical Oils

Brownley, Charles A.; Lachman, Leon

doi:10.1002/jps.2600541017

Cited by 9 publications

(3 citation statements)

References 18 publications

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“…9 shows a MA-TBA adduct which has a UV absorption at 535 nm. Later, the MA-TBA adduct was measured by a spectrophotometer to assess antioxidant effectiveness in pharmaceutical oils [166]. Consequently, the MA-TBA assay became the most popular method for studies related to lipid peroxidation.…”

Section: Conventional Methods For Ma Determinationmentioning

confidence: 99%

Analytical methods for trace levels of reactive carbonyl compounds formed in lipid peroxidation systems

Shibamoto

2006

Journal of Pharmaceutical and Biomedical Analysis

121

107

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Section: Conventional Methods For Ma Determinationmentioning

confidence: 99%

Analytical methods for trace levels of reactive carbonyl compounds formed in lipid peroxidation systems

Shibamoto

2006

Journal of Pharmaceutical and Biomedical Analysis

121

107

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“…TBA assay used 2-thiobarbituric acid to interact with the lipid oxidation product malonaldehyde to form a pink compound, with a maximum absorption value at 532 nm (Figure 6). The test result was usually expressed as the product of the absorbance at 532 nm and the absorption coefficient of malonaldehyde, which is commonly referred to as the TBA value (Brownley, & Lachman, 1965).…”

Section: Tba Assaymentioning

confidence: 99%

Guidelines for antioxidant assays for food components

et al. 2020

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Recently, research on antioxidants has become increasingly active in various fields.Antioxidants react through free radical or molecular oxygen quenching, being capable to either delay or inhibit the oxidation processes that occur under the influence of molecular oxygen or reactive oxygen species. Accordingly, assays developed to evaluate the antioxidant activity of food constituents vary. Therefore, to investigate the antioxidant activity of chemical(s), choosing an adequate assay based on the properties of chemical(s) is critical. Antioxidant assays may be broadly classified as electron transfer (ET)-based assays and hydrogen atom transfer (HAT)-based assays. ET-based assays include ABTS assay, DPPH assay, ferrous oxidation-xylenol orange assay, ferric thiocyanate assay, ferric reducing/antioxidant power assay, potassium ferricyanide reducing power assay, and cupric reducing antioxidant power assay. HAT-based assays include oxygen radical absorbance capacity assay, total peroxyl radical-trapping antioxidant parameter assay, thiobarbituric acid assay, -carotene bleaching assay, and cellular antioxidant activity assay. In this guideline, assays used recently were selected for extended discussion, including the mechanisms underlying each assay as well as the practice of antioxidant capacity assessment.

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“…In the late 1950s, estimation of 2-deoxy sugars was conducted using the MA-TBA product (Figure 2), which was monitored by a colorimeter at UV absorption of 535 nm (101). Later, this method was used with a spectrophotometer to assess antioxidant effectiveness in pharmaceutical oils (102). Consequently, the MA-TBA assay became one of the most popular assays for studies related to lipid peroxidation, and it is still currently used widely to evaluate antioxidant activities of various natural products.…”

Section: Assays Associated With Lipid Peroxidationsmentioning

confidence: 99%

Antioxidant Assays for Plant and Food Components

Moon

Shibamoto

2009

J. Agric. Food Chem.

797

534

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Recently, research on natural antioxidants has become increasingly active in various fields. Accordingly, numerous articles on natural antioxidants, including polyphenols, flavonoids, vitamins, and volatile chemicals, have been published. Assays developed to evaluate the antioxidant activity of plants and food constituents vary. Therefore, to investigate the antioxidant activity of chemical(s), choosing an adequate assay based on the chemical(s) of interest is critical. There are two general types of assays widely used for different antioxidant studies. One is an assay associated with lipid peroxidations, including the thiobarbituric acid assay (TBA), malonaldehyde/high-performance liquid chromatography (MA/HPLC) assay, malonaldehyde/gas chromatography (MA/GC) assay, beta-carotene bleaching assay, and conjugated diene assay. Other assays are associated with electron or radical scavenging, including the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, ferric reducing/antioxidant power (FRAP) assay, ferrous oxidation-xylenol orange (FOX) assay, ferric thiocyanate (FTC) assay, and aldehyde/carboxylic acid (ACA) assay. In this review, assays used recently were selected for extended discussion, including discussion of the mechanisms underlying each assay and its application to various plants and foods.

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Use of 2-Thiobarbituric Acid—Malonaldehyde Reaction as a Measure of Antioxidant Effectiveness in Pharmaceutical Oils

Cited by 9 publications

References 18 publications

Analytical methods for trace levels of reactive carbonyl compounds formed in lipid peroxidation systems

Analytical methods for trace levels of reactive carbonyl compounds formed in lipid peroxidation systems

Guidelines for antioxidant assays for food components

Antioxidant Assays for Plant and Food Components

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