Use of a free radical method to evaluate antioxidant activity

Brand-Williams, Wendy; Cuvelier, Marie-Elisabeth; Berset, Claudette

doi:10.1016/s0023-6438(95)80008-5

Cited by 19,000 publications

(11,695 citation statements)

References 8 publications

Supporting

104

Mentioning

11,040

Contrasting

Unclassified

540

Order By: Relevance

“…Butylatedhydroxytoluene (BHT) was used as positive control. The concentration providing 50% radical inhibition (IC 50 ) was calculated from the graph of inhibition percentage plotted against honey concentrations (Brand‐Williams, Cuvelier, & Berset, 1995). …”

Section: Methodsmentioning

confidence: 99%

Physicochemical characterization and antioxidant activity of Palestinian honey samples

Imtara

Elamine

Lyoussi

2018

Food Science & Nutrition

View full text Add to dashboard Cite

Physicochemical characteristics, main minerals, and antioxidant activity were determined for Palestinian honey samples belonging to different floral and geographical origins. The features of the analyzed samples were within the established international standards for honey quality control. One clear exception was the hydroxymethylfurfural (HMF) of the Ziziphus sample purchased from the Jericho region, which is the lowest city in the word characterized by a hot desert climate. The observed HMF value was 81.86 ± 2.64 mg/kg being two folds the maximum allowed in honey samples (40 mg/kg). As a second objective of the present work, the parameters were divided into two groups with different discriminatory power. The assessed physicochemical parameters, and the antioxidant activities, specific to the botanical origin discrimination, were used to run the first PCA. A strong correlation could be seen between the bioactive compounds and the antioxidant activities despite the geographical origin of the samples. Thyme and Ziziphus samples were the best samples, while citrus sample presented the lowest activity. Regarding the geographical discrimination, Ash and mineral contents in addition to the electrical conductivity were used. The output PCA conserved high represent ability of the data in the two‐first components being 82.72% and 9.60%. A little discrimination between the samples produced in the north and those produced in the south of the country, but it was not perfect. The intervention of the botanical variability could be the reason.

show abstract

Section: Methodsmentioning

confidence: 99%

Physicochemical characterization and antioxidant activity of Palestinian honey samples

Imtara

Elamine

Lyoussi

2018

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…This method is limited because DPPH radical interacts with other radicals and the time response curve to reach the steady state is not linear with different ratios of antioxidant/ DPPH (Brand-Williams et al 1995;Sanchez-Moreno et al 1998). DPPH is sensitive to some Lewis bases and solvent types as well as oxygen (Ancerewicz et al 1998).…”

Section: Dpph Assaymentioning

confidence: 99%

Genesis and development of DPPH method of antioxidant assay

2011

View full text Add to dashboard Cite

α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period. However, with the advancement and sophistication in instrumental techniques, the method has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them. This article presents a critical review on various developments to the DPPH method.

show abstract

“…Brand-William et al established the DPPH method (Brand-Williams et al 1995) and it has been widely used to test free radical-scavenging ability of various samples in present studies. The DPPH radical scavenging activities of the extracts and fractions were dose-dependent ( Fig.…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai

et al. 2011

View full text Add to dashboard Cite

The antioxidant potency of various extracts and fractions from the leaves and stem of Epimedium koreanum Nakai was evaluated using three esteblished methods, specifically the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radica-scavenging activity assay, the inhibitory effect on lipid peroxidation induced by Fe 2+ /ascorbate (MDA) assay and the ferric reducing power (FRP) assay. The amounts of total phenolics and total flavonoids in the extracts and fractions were determined by spectrophotometric methods and the content of icariin was determined by HPLC. The results showed that all the extracts and fractions exhibited antioxidant activities at different magnitudes of potency. The leaf extract and fractions demonstrated superior antioxidant activity in most of the assays. The decreasing order of antioxidant activities among the extracts/fractions assayed through the three methods were found to be n-BuOH fraction>ethyl acetate fraction>ethanol extract>petroleum ether fraction>-water fraction. A positive correlation was found between the amounts of total phenolics, total flavonoids and icariin and DPPH radical scavenging activity (R 2 =0.9935, 0.9944 and 0.9997, respectively) and inhibitory activity on lipid peroxidation (R 2 =0.9987, 0.9830 and 0.9886, respectively). The results suggested that Icariin was one of the main constituents contribute to the antioxidant activity of Epimedium koreanum Nakai and the n-BuOH fractions of leaf extract might be valuable antioxidant natural sources.

show abstract

Use of a free radical method to evaluate antioxidant activity

Cited by 19,000 publications

References 8 publications

Physicochemical characterization and antioxidant activity of Palestinian honey samples

Physicochemical characterization and antioxidant activity of Palestinian honey samples

Genesis and development of DPPH method of antioxidant assay

Comparative studies on antioxidant activities of extracts and fractions from the leaves and stem of Epimedium koreanum Nakai

Contact Info

Product

Resources

About