Use of a Model of a Blood-Induced Bruise for the Evaluation of Formulations on Bruising

Abstract

Esthetic treatments can induce swelling and bruises. Thus, a treatment that would prevent or hasten the resolution of bruising should be very useful. Generally, the regression of bruising was conducted with patients or animals models. So we decided firstly to develop an ex vivo model in order to test antibruising properties of topical formulations and secondly to evaluate a curative effect of a cream (mixture of arnica extract and apigenin) in comparison with a positive control (vulnerary cream) and also to estimate the preventive interest of this cream. The results showed that the injection of 25 µl of blood into the dermis of skin fragments was sufficient to create a model of induced-bruise. The duration of 24 hours was chosen to compare the effects of actives on the decrease in the size of the bruise. Joint effects of a pretreatment and a treatment of a mixture of arnica extract and apigenin decreased significantly the area of bruising compared to the treatment group, the control group and the positive control group. Many topical products claim to improve bruising on their package label. Our model can demonstrate their efficacy and determinate the best topical antibruising formulation. The mechanism involved in anti-inflammatory activity of active compounds of topical formulations is often not fully understood. Our blood-induced model may bring some responses through the study of mediators of the inflammation.

Gambaran Hematologi Tikus setelah Pemberian Terapi Gel Ekstrak Kasar Bromelin Kulit Nanas (Ananas comosus (L.) Merr)

“…Ketika ini terjadi, darah dari kapiler yang pecah di dekat permukaan kulit keluar dengan keluar di bawah kulit. Karena tidak ada tempat untuk pergi, darah terperangkap, membentuk tanda merah atau keunguan yang lembut saat disentuh (Robin et al, 2015). Pertama, warnanya kemerahan.…”

“…Warna awal merah pada memar adalah produk pigmentasi alami kulit, warna pigmen dalam darah yang diekstravasasi, dan warna apa pun yang ditambahkan oleh reaksi inflamasi (misalnya kemerahan akibat vasodilatasi), tetapi dengan berjalannya waktu warna memar akan berubah dari warna merah menjadi hitam kebiruan. Kemudian setelah beberapa hari, menjadi agak hijau (setelah 4 sampai 7 hari), kuning atau coklat (setelah 7 hari) (Robin et al, 2015).…”