Use of A New HPLC Method in Rat Liver Microsomal Testosterone Monooxygenation and Its Application to Study the Sex Dependent Expression of Several Hydroxylases

Gil, Lionel; Orellana, Miriam; Mancilla, Jerson; García, Juan Pablo; Vásquez, Hernán

doi:10.1080/00032718608064552

Cited by 5 publications

(1 citation statement)

References 25 publications

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“…Substrates for this system can be either endogenous substances such as fatty acids, steroid hormones and prostaglandins (1,2) or exogenous xenobiotics such as drugs, chemical carcinogens and environmental pollutants.…”

Section: Introductionmentioning

confidence: 99%

A novel isocratic HPLC method to separate and quantify acetanilide and its hydroxy aromatic derivatives: 2-, 3- and 4-hydroxyacetanilide (paracetamol or acetaminophen)

Mancilla

Valdés

Gil

1989

European Journal of Drug Metabolism and Pharmacokinetics

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Reverse-phase high performance liquid chromatography on a microBondapak C-18 Column has been used to separate and quantify acetanilide and its aromatic monohydroxy derivatives in the 2-, 3- and 4- positions. Separation was achieved within 22 min by using an isocratic mixture of 2-propanol: methanol: water, 8:18:74 (v/v). This method compares very favourably with other HPLC techniques already reported to separate acetanilide from the monohydroxy aromatic derivatives.

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Section: Introductionmentioning

confidence: 99%

A novel isocratic HPLC method to separate and quantify acetanilide and its hydroxy aromatic derivatives: 2-, 3- and 4-hydroxyacetanilide (paracetamol or acetaminophen)

Mancilla

Valdés

Gil

1989

European Journal of Drug Metabolism and Pharmacokinetics

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Dietary fish oil and cytochrome P‐450 monooxygenase activity in rat liver and kidney

Valdes

Vega

Avalos

et al. 1995

Lipids

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Lauric acid hydroxylation and aminopyrine N-demethylation were studied in kidney and liver microsomes from rats treated with fish oil. Different doses of fish oil containing 20% eicosapentaenoic acid and 10% docosahexaenoic acid were provided daily to the rats for seven days. In all the groups studied, the lauric acid metabolism was higher in kidney microsomes and the aminopyrine metabolism in the liver microsomes. Although no effect on the renal cytochrome P-450 concentration was detectable, all four fish oil doses increased the hepatic concentration of cytochrome P-450 by a mean 27%. The higher fish oil doses used increased the renal and hepatic microsomal metabolism of aminopyrine. The lauric acid metabolism was increased by fish oil only in the liver. Fish oil, a known inducer of fatty acid peroxisomal beta-oxidation, also induced microsomal activity. These results show that liver and kidney respond in different ways to dietary factors such as fish oil. In addition, our study would suggest that fish oil increased the activity of two different families of liver cytochrome P-450. The activity of kidney lauric acid 11- and 12-hydroxylation, however, was not modulated by fish oil.

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Modulation of rat liver peroxisomal and microsomal fatty acid oxidation by starvation

et al. 1992

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In this work the micro.somal laurie acid a~-hydroxylation, fatty acid peroxisomalfl-oxidation, and the levels ofcytochrome P-450 IVAI were studied in liver tissu¢ from ,starved rats. Starvation increased the peroxisomal S-oxidation and the microsomal hydroxylation of fatty acids. The correlation between these activities would .support the proposal that both processes are linked, contributing in part to catabolism of fatty acids in liver of starvgl rats.

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Use of A New HPLC Method in Rat Liver Microsomal Testosterone Monooxygenation and Its Application to Study the Sex Dependent Expression of Several Hydroxylases

Cited by 5 publications

References 25 publications

A novel isocratic HPLC method to separate and quantify acetanilide and its hydroxy aromatic derivatives: 2-, 3- and 4-hydroxyacetanilide (paracetamol or acetaminophen)

A novel isocratic HPLC method to separate and quantify acetanilide and its hydroxy aromatic derivatives: 2-, 3- and 4-hydroxyacetanilide (paracetamol or acetaminophen)

Dietary fish oil and cytochrome P‐450 monooxygenase activity in rat liver and kidney

Modulation of rat liver peroxisomal and microsomal fatty acid oxidation by starvation

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