Use of a rat ex-vivo testis culture method to assess toxicity of select known male reproductive toxicants

Goldstein, Keith M.; Seyler, David E.; Durand, Philippe; Perrard, Marie-Hélène; Baker, Thomas K.

doi:10.1016/j.reprotox.2016.01.003

Cited by 19 publications

(12 citation statements)

References 75 publications

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“…The mechanisms of the association between BPA and decreasing sperm concentration have been well documented. Studies have shown that BPA may target Sertoli cells and block the meiotic progression of germ cells, which in turn have a direct adverse impact on spermatogenesis 40 . In addition, BPA, acting as an androgen receptor (AR) antagonist, interrupts the normal AR binding activity and the interaction between AR and endogenous androgens 41 .…”

Section: Discussionmentioning

confidence: 99%

Exposure of environmental Bisphenol A in relation to routine sperm parameters and sperm movement characteristics among fertile men

Miao

Liang

et al. 2018

Sci Rep

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Although several human studies have examined bisphenol A (BPA) exposure in relation to routine sperm parameters, evidence of BPA’s effects on sperm movement characteristics is limited. We examined associations of BPA exposure with sperm parameters including sperm movement characteristics among fertile men. The cross-sectional study was conducted in Sandu County, Guizhou Province, China. Subjects provided semen samples analyzed by computer-aided sperm analysis (CASA) system and urine samples for BPA assay. They were invited to complete an in-person interview with a structured questionnaire to obtain demographics, lifestyle factors, etc. In final analyses, 500 subjects were included. We used multivariate linear regression analyses to estimate associations between BPA and sperm parameters after adjusting for potential confounders. BPA was detected in 73.6% of urine samples, with a geometric mean of 0.44 μg/gCreatinine. Compared with subjects of undetected BPA, subjects with detected BPA had increased Linearity (LIN, β: 2.19, 95% confidence interval (CI): 0.37, 4.0), Straightness (STR, β: 1.47, 95% CI: 0.19, 2.75), Wobble (WOB, β: 1.75, 95% CI: 0.26, 3.25), reduced Amplitude of lateral head displacement (ALH, β: −0.26, 95% CI: −0.5, −0.02) and Mean angular displacement (MAD, β: −2.17, 95% CI: −4.22, −0.11). Subjects in the highest tertile of creatinine-adjusted BPA group had lower sperm concentration than those with undetected BPA. Dose-response relationships of BPA with LIN, WOB, ALH, MAD and sperm concentration were demonstrated by statistically significant trends across tertiles of creatinine-adjusted BPA concentrations. Similar results were obtained using unadjusted BPA concentrations. Exposure to environmental BPA would decrease sperm concentration and sperm swing characteristics (ALH and MAD), and increase sperm velocity ratios (LIN, STR and WOB), which might mediate further effects on impaired male fecundity.

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Section: Discussionmentioning

confidence: 99%

Exposure of environmental Bisphenol A in relation to routine sperm parameters and sperm movement characteristics among fertile men

Miao

Liang

et al. 2018

Sci Rep

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“…As noted by Tiwari and Vanage () BPA might act as a mutagen in male germ cells and cause DNA damage in sperm. BPA targeted Sertoli cells and blocked the meiotic progression of germ cells (Goldstein et al ., ). In addition, D'Cruz et al .…”

Section: Effect Of Bisphenol a On Male Reproductive Processes And Fermentioning

confidence: 97%

“…As noted by Tiwari and Vanage (2013) BPA might act as a mutagen in male germ cells and cause DNA damage in sperm. BPA targeted Sertoli cells and blocked the meiotic progression of germ cells (Goldstein et al, 2016). In addition, D'Cruz et al (2012b found decreased GLUT-8 expression in the testis in spermatocytes and developing spermatids of rats exposed to low BPA.…”

Section: Effect Of Bisphenol a On Male Reproductive Processes And Fermentioning

confidence: 99%

Effect of bisphenol A on reproductive processes: A review of in vitro, in vivo and epidemiological studies

Tomza–Marciniak

Stępkowska

Kuba

et al. 2017

J of Applied Toxicology

118

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As bisphenol A (BPA) is characterized by a pronounced influence on human hormonal regulation, particular attention has been aimed at understanding its role in reproductive processes in males and females, as well as on fetal development. Owing to the increasing number of alarming reports on the negative consequences of the presence of BPA in human surroundings, more and more studies are being undertaken to clarify the negative effects of BPA on human reproductive processes. The aim of this work was to collect and summarize data on the influence of BPA exposure on reproductive health. Based on an analysis of selected publications it was stated that there is strong proof confirming that BPA is an ovarian, uterine and prostate toxicant at a level below the lowest observed adverse effect level (50 mg kg À1 bodyweight) as well as a level below the proposed safe level (4 μg kg À1 bodyweight). It seems there is also reliable evidence in relation to the negative effect of BPA on sperm quality and motility. Limited evidence also pertains to the case of the potential of BPA to affect polycystic ovary syndrome occurrence. Although in epidemiological studies this disease was common, in studies on animal models such results were still not confirmed. No unambiguous results of epidemiological studies and with animal models were obtained in relation to the evaluation of associations between BPA and implantation failure in women, evaluation of associations between BPA and sexual dysfunction in men, and impact of BPA on birth rate, birth weight and length of gestation.

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“…As an AR antagonist, BPA could block the normal binding activity and its interaction between AR and endogenous androgen, and thus impaired the normal spermatogenesis [ 55 ]. Concomitantly, BPA may have a direct adverse impact on spermatogenesis by targeting Sertoli cells and interrupting the meiotic progression of germ cells [ 56 ]. Also, some experi-mental studies stated that BPA could inhibit the sperm mobility, which might be mediated by means of compromising mitochondrial functions (increase the mitochondrial ROS or reduce the high mitochondrial inner transmembrane potential) and decreasing ATP levels in spermatozoa [ 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

Early-life exposure to bisphenol A and reproductive-related outcomes in rodent models: a systematic review and meta-analysis

Ren

Zhang

Chen

et al. 2020

Aging

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We performed this meta-analysis to elucidate the associations between early-life BPA exposure and reproductive-related outcome indicators. The standardized mean differences (SMDs) and its 95% confidence intervals (CIs) were measured by fixed-effects or random-effects models. The results revealed that BPA exposure at extremely-high dose (>50mg/kg/day) was significantly associated with negative reproductive-related outcomes (Prostate weight: SMD: -4.21; 95% Cl: -5.97, -2.44; Testis weight: SMD: -1.92; 95% Cl: -2.61, -1.23; Epididymis weight: SMD: -2.16; 95% Cl: -3.47, -0.86; Daily sperm production; SMD: -1.90; 95% Cl: -3.27, -0.53; Epididymal sperm count; SMD: -3.42; 95% Cl: -3.87, -2.97). Meanwhile, regardless of the dose, early-life BPA exposure could result in an adverse effect on sperm parameters of F1 generation male rodents at any period. Also, we found the non-monotonic dose response curves of BPA in specific tissues or organs, which may challenge the traditional mindset of "safe dose". This study demonstrated that bisphenol A exposure was relevant to adverse reproductive-related outcomes at specially appointed dose and period of life. Yet the assumption that no adverse effects can occur below the "safe" dose is suspected.

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Use of a rat ex-vivo testis culture method to assess toxicity of select known male reproductive toxicants

Cited by 19 publications

References 75 publications

Exposure of environmental Bisphenol A in relation to routine sperm parameters and sperm movement characteristics among fertile men

Exposure of environmental Bisphenol A in relation to routine sperm parameters and sperm movement characteristics among fertile men

Effect of bisphenol A on reproductive processes: A review of in vitro, in vivo and epidemiological studies

Early-life exposure to bisphenol A and reproductive-related outcomes in rodent models: a systematic review and meta-analysis

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