Use of a Solvent-Free Dry Matrix Coating for Quantitative Matrix-Assisted Laser Desorption Ionization Imaging of 4-Bromophenyl-1,4-diazabicyclo(3.2.2)nonane-4-carboxylate in Rat Brain and Quantitative Analysis of the Drug from Laser Microdissected Tissue Regions

Goodwin, Richard J. A.; Scullion, Paul; MacIntyre, Lynsey; Watson, David; Pitt, Andrew R.

doi:10.1021/ac100398y

Cited by 64 publications

(53 citation statements)

References 25 publications

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“…In this context, the matrix application protocol is also critical. Usually, solvent-free deposition methods are advisable [18][19][20][21], as they ensure that the molecules in the tissue are not perturbed during this step. So far, matrix sublimation and the new ion implanters used by some groups seem to yield the best results [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Imaging mass spectrometry increased resolution using 2-mercaptobenzothiazole and 2,5-diaminonaphtalene matrices: application to lipid distribution in human colon

et al. 2015

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Imaging mass spectrometry is becoming a reference technique in the field of lipidomics, due to its ability to map the distribution of hundreds of species in a single run, along a tissue section. The next frontier is now achieving increasing resolution powers to offer cellular (or even sub-cellular) resolution. Thus, the new spectrometers are equipped with sophisticated optical systems to decrease the laser spot to <30 μm. Here, we demonstrate that by using the correct matrix (i.e., a matrix that maximizes ion detection and forms small crystals) and a careful preparation, it is possible to achieve resolutions of ∼5-10 μm, even with spectrometers equipped with non-optimal optics, which produces laser spots of 50 μm or even larger. As a proof of concept, we present images of distributions of lipids, both in positive and negative ion mode, over human colon endoscopic sections, recorded using 2-mercaptobenzothiazole for positive ion mode and 2,5-diaminonaphtalene for negative ion mode and an LTQ-Orbitrap XL, equipped with a matrix-assisted laser desorption ionization (MALDI) source that produces astigmatic laser spots. Graphical Abstract Imaging mass spectrometry is becoming an invaluable technique to complement traditional histology, but still higher resolutions are required. Here we deal with such issue.

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Section: Introductionmentioning

confidence: 99%

Imaging mass spectrometry increased resolution using 2-mercaptobenzothiazole and 2,5-diaminonaphtalene matrices: application to lipid distribution in human colon

et al. 2015

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“…The matrix density on the sections is controlled by direct observation using a standard light microscope. This matrix deposition approach was also found to be ideal to image certain drug compounds (88,89). Both strategies allow very rapid production of homogeneous matrix coatings on tissue sections with a fairly inexpensive setup.…”

mentioning

confidence: 99%

From Whole-body Sections Down to Cellular Level, Multiscale Imaging of Phospholipids by MALDI Mass Spectrometry

Chaurand¹,

Cornett²,

Angel

et al. 2011

Molecular & Cellular Proteomics

120

137

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Significant progress in instrumentation and sample preparation approaches have recently expanded the potential of MALDI imaging mass spectrometry to the analysis of phospholipids and other endogenous metabolites naturally occurring in tissue specimens. Here we explore some of the requirements necessary for the successful analysis and imaging of phospholipids from thin tissue sections of various dimensions by MALDI time-of-flight mass spectrometry. We address methodology issues relative to the imaging of whole-body sections such as those cut from model laboratory animals, sections of intermediate dimensions typically prepared from individual organs, as well as the requirements for imaging areas of interests from these sections at a cellular scale spatial resolution. We also review existing limitations of MALDI imaging MS technology relative to compound identification. Finally, we conclude with a perspective on important issues relative to data exploitation and management that need to be solved to maximize biological understanding of the tissue specimen investigated. Molecular & Cellular Proteomics 10: 10.1074/mcp.O110.004259, 1-11, 2011.Since its introduction in the late 90s (1), MALDI imaging mass spectrometry (MS) technology has witnessed a phenomenal expansion. Initially introduced for the mapping of intact proteins from fresh frozen tissue sections (2), imaging MS is now routinely applied to a wide range of different compounds including peptides, proteins, lipids, metabolites, and xenobiotics (3-7). Numerous compound-specific sample preparation protocols and analytical strategies have been developed. These include tissue sectioning and handling (8 -14), automated matrix deposition approaches and data acquisition strategies (15-21), and the emergence of in situ tissue chemistries (22)(23)(24)(25). Originally performed on sections cut from fresh frozen tissue specimens, methodologies incorporating an in situ enzymatic digestion step prior to matrix application have been optimized to access the proteome locked in formalin-fixed paraffin-embedded tissue biopsies (25)(26)(27)(28)(29). The possibility to use tissues preserved using non-cross-linking approaches has also been demonstrated (30 -32). These methodologies are of high importance for the study of numerous diseases because they potentially allow the retrospective analysis for biomarker validation and discovery of the millions of tissue biopsies currently stored worldwide in tissue banks and repositories.In the past decade, instrumentation for imaging MS has also greatly evolved. Whereas the first MS images were collected with time-of-flight instruments (TOF) capable of repetition rates of a few hertz, modern systems are today capable of acquiring data in the kilohertz range and above with improved sensitivity, mass resolving power, and accuracy, significantly reducing acquisition time and improving image quality (33, 34). Beyond time-of-flight analyzers, other MALDIbased instruments have been used such as ion traps (35-37), Qq TOF instruments (38 -40), and ...

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“…The authors claim that the use of a solvent-free dry-matrix coating is an alternative to wet-matrix coating for the analysis of small molecules by MALDI-IMS. By doing so, it is possible to detect the compound of interest, which could not be detected when conventional wet-matrix methods were used [41]. Pseudomultiple reaction monitoring was used to validate the distribution of the target compound [41].…”

Section: Quantitation Methods For Imsmentioning

confidence: 99%

Probe-based chemical modulations of tissues for IMS

Santos

Capelo

2012

Journal of Proteomics

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Use of a Solvent-Free Dry Matrix Coating for Quantitative Matrix-Assisted Laser Desorption Ionization Imaging of 4-Bromophenyl-1,4-diazabicyclo(3.2.2)nonane-4-carboxylate in Rat Brain and Quantitative Analysis of the Drug from Laser Microdissected Tissue Regions

Cited by 64 publications

References 25 publications

Imaging mass spectrometry increased resolution using 2-mercaptobenzothiazole and 2,5-diaminonaphtalene matrices: application to lipid distribution in human colon

Imaging mass spectrometry increased resolution using 2-mercaptobenzothiazole and 2,5-diaminonaphtalene matrices: application to lipid distribution in human colon

From Whole-body Sections Down to Cellular Level, Multiscale Imaging of Phospholipids by MALDI Mass Spectrometry

Probe-based chemical modulations of tissues for IMS

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