Use of a Super-hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-organoids

Brevini, Tiziana A. L.; Manzoni, Elena F. M.; Ledda, Sergio; Gandolfi, F.

doi:10.1007/7651_2017_47

Cited by 9 publications

(7 citation statements)

References 13 publications

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“…Encapsulation of cells in microbioreactor material with hydrophobic properties may further boost the formation of functional organoids that are not only protected by the supporting surface, but are also provided with optimal gas exchange between the interior liquid and the surrounding environment (Fig. 3; Arbatan et al 2012;Sarvi et al 2013Sarvi et al , 2015Tian et al 2013;Serrano et al 2015;Brevini et al 2017). Together, accumulating evidence indicates that, similar to soluble growth factors, cell morphology and the mechanical properties of the cellular microenvironment can affect both cell growth and cell differentiation.…”

Section: Mechanical Signals Control Cell Fatementioning

confidence: 99%

Methylation mechanisms and biomechanical effectors controlling cell fate

Brevini

Manzoni

Gandolfi

2018

Reprod. Fertil. Dev.

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Mammalian development and cell fate specification are controlled by multiple regulatory mechanisms that interact in a coordinated way to ensure proper regulation of gene expression and spatial restriction, allowing cells to adopt distinct differentiation traits and a terminal phenotype. For example, cell potency is modulated by changes in methylation that are under the control of methyltransferases and ten–eleven translocation (TET) enzymes, which establish or erase a phenotype-specific methylation pattern during embryo development and mesenchymal to epithelial transition (MET). Cell plasticity is also responsive to extracellular factors, such as small molecules that interact with cell fate definition and induce a transient pluripotent state that allows the direct conversion of an adult mature cell into another differentiated cell type. In addition, cell-secreted vesicles emerge as powerful effectors, capable of modifying cell function and phenotype and delivering different signals, such as octamer-binding transcription factor-4 (Oct4) and SRY (sex determining region Y)-box 2 (Sox2) mRNAs (implicated in the preservation of pluripotency), thus triggering epigenetic changes in the recipient cells. In parallel, mechanical properties of the cellular microenvironment and three-dimensional rearrangement can affect both cell potency and differentiation through marked effects on cytoskeletal remodelling and with the involvement of specific mechanosensing-related pathways.

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Section: Mechanical Signals Control Cell Fatementioning

confidence: 99%

Methylation mechanisms and biomechanical effectors controlling cell fate

Brevini

Manzoni

Gandolfi

2018

Reprod. Fertil. Dev.

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“…Thanks to all these unique properties, together with the easy manipulation and the high reproducibility and efficiency, liquid marbles have found numerous applications in the field of microfluidics, storage, transportation, and mechanical computation [ 73 , 74 , 75 , 76 ], as well as in the chemical and biological areas [ 77 ]. Recently, several studies have pointed to micro-bioreactors as ideal platforms, essential for biomedical applications and culture of microorganisms or cells of different types, spanning from oocyte [ 78 ], cardiac [ 79 ], pancreatic [ 80 ], toroid [ 81 ], and olfactory ensheathing [ 82 ] to high plasticity cells [ 32 ]. In particular, we demonstrated that cell encapsulation in micro-bioreactors induces distinctive 3D cell rearrangements and specific cell-to-cell interactions [ 32 ].…”

Section: D Cell Culture Systemsmentioning

confidence: 99%

Current Advances in 3D Tissue and Organ Reconstruction

Pennarossa

Arcuri

Iorio

et al. 2021

IJMS

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Bi-dimensional culture systems have represented the most used method to study cell biology outside the body for over a century. Although they convey useful information, such systems may lose tissue-specific architecture, biomechanical effectors, and biochemical cues deriving from the native extracellular matrix, with significant alterations in several cellular functions and processes. Notably, the introduction of three-dimensional (3D) platforms that are able to re-create in vitro the structures of the native tissue, have overcome some of these issues, since they better mimic the in vivo milieu and reduce the gap between the cell culture ambient and the tissue environment. 3D culture systems are currently used in a broad range of studies, from cancer and stem cell biology, to drug testing and discovery. Here, we describe the mechanisms used by cells to perceive and respond to biomechanical cues and the main signaling pathways involved. We provide an overall perspective of the most recent 3D technologies. Given the breadth of the subject, we concentrate on the use of hydrogels, bioreactors, 3D printing and bioprinting, nanofiber-based scaffolds, and preparation of a decellularized bio-matrix. In addition, we report the possibility to combine the use of 3D cultures with functionalized nanoparticles to obtain highly predictive in vitro models for use in the nanomedicine field.

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“…Optical metabolic imaging is highly sensitive in revealing metabolic alterations 1–2 h following treatment with effective drugs, and such changes correlate with the expected response (i.e., HER2 expression in breast cancer) [ 105 ]. Further, optical metabolic imaging distinguishes cell types and drug response [ 18 , 29 , 41 , 62 , 63 , 106 , 107 ]. For instance, fibroblasts from PDAC organoids show drug response, despite no overt cell death enhancement [ 94 ].…”

Section: Applications Of Ptosmentioning

confidence: 99%

A pancreas tumor derived organoid study: from drug screen to precision medicine

et al. 2021

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Pancreatic ductal adenocarcinoma (PDAC) one of the deadliest malignant tumor. Despite considerable progress in pancreatic cancer treatment in the past 10 years, PDAC mortality has shown no appreciable change, and systemic therapies for PDAC generally lack efficacy. Thus, developing biomarkers for treatment guidance is urgently required. This review focuses on pancreatic tumor organoids (PTOs), which can mimic the characteristics of the original tumor in vitro. As a powerful tool with several applications, PTOs represent a new strategy for targeted therapy in pancreatic cancer and contribute to the advancement of the field of personalized medicine.

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Use of a Super-hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-organoids

Cited by 9 publications

References 13 publications

Methylation mechanisms and biomechanical effectors controlling cell fate

Methylation mechanisms and biomechanical effectors controlling cell fate

Current Advances in 3D Tissue and Organ Reconstruction

A pancreas tumor derived organoid study: from drug screen to precision medicine

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