Use of a Tissue Culture Method to Develop New Forms of Fagopyrum esculentum Moench

Барсукова, Е. Н.; Клыков, А. Г.; Чайкина, Е. Л.

doi:10.3103/s1068367419060028

Cited by 2 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is interesting to note that the minimum accumulation of flavonoid was observed after exposure to the highest test concentration of 1313 mg L −1 zinc salt, which was 3.32%, which is 1.2 times as low as that in the control group. An increase of flavonoid rutin biosynthesis in buckwheat (1.25-time higher compared with the control group) under the effect of elevated zinc salt concentration (404 mg L −1 ) in vitro was demonstrated in our earlier works [2].…”

Section: Resultsmentioning

confidence: 56%

“…This feature of buckwheat is emphasized by I. Kreft [42]. Tolerance of buckwheat microstems to elevated concentrations of copper and zinc sulfate in vitro was mentioned earlier [2]. According to M. Germ and A. Gaberščik [43], different species and varieties of buckwheat, originating from relatively harsh habitats [44], can be classified as the most stress-resistant crops.…”

Section: Resultsmentioning

confidence: 99%

“…Plant breeding using heavy metal ions as selective backgrounds in vitro can be an effective method to create breeding material tolerant to abiotic stresses [1][2][3]. Furthermore, stress effects trigger the immune mechanisms of plants inducing an increase in flavonoid biosynthesis [4,5], which have great therapeutic and preventive value for human health and contribute to reduced occurrence of many diseases [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…For example, regenerant plants of bentgrass Agrostis stolonifera tolerant to cadmium, lead, copper, and zinc were created using heavy metal ions [12][13][14], in vitro zinc-resistant starting material for Brassica campestris and Brassica juncea [15], Setaria italis [16], and Nicotiana tabacum [17]. Selective media with elevated concentrations of zinc (46-606 mg L −1 ) and copper (6-230 mg L −1 ) were previously used for cell, tissue and organ culture in buckwheat [2,3,18,19].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Study of the Effect of Selective Media with High Doses of Zinc on Regeneration Ability and Rutin Accumulation in Common Buckwheat In Vitro

Borovaya¹,

Клыков²,

Барсукова³

et al. 2022

Plants

View full text Add to dashboard Cite

Biotechnological methods are widely used in modern common buckwheat (Fagopyrum esculentum Moench) studies, constitute an effective tool to create the best agronomic traits of the crop, and can also be used to breed forms, resistant to heavy metal ion toxicity, which is important in the environment of constantly growing anthropogenic pressure on ecosystems. The studied high concentrations of zinc salts (808–1313 mg L−1) in the nutrient medium in vitro had an inhibitory effect on buckwheat, which was manifested by a decrease in values of its morphological indicators. Ion stress had an adverse effect on 7–9% of plants from their total number, indicating high plasticity and resistance of F. esculentum to highly toxic doses of zinc. The stress state of F. esculentum significantly increases the production of flavonoid compounds, including rutin, in plant cells, which is used in biotechnology to assess and obtain buckwheat forms of high flavonoid induction capacity. The processes of rutin biosynthesis were most intense in test-tube plants of the Izumrud × Inzerskaya hybrid obtained after exposure to high doses of zinc 1010–1212 mg L−1. F. esculentum genotypes obtained using selective backgrounds with high zinc concentrations are promising biosystems for synthesis of rutin, valuable for pharmacology and medicine.

show abstract

Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Study of the Effect of Selective Media with High Doses of Zinc on Regeneration Ability and Rutin Accumulation in Common Buckwheat In Vitro

Borovaya¹,

Клыков²,

Барсукова³

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

Using plant extracts for the micropropagation of buckwheat

Borovaya,

Boginskaya,

Klykov

2023

Ovoŝi Rossii

Self Cite

View full text Add to dashboard Cite

Background. Various plant hormones are used (cytokinins, auxins) to increase the regeneration efficiency and the net reproduction rate of buckwheat in vitro. However, the growth and development rates of plantlets have been noted to be low under these conditions. For this reason, search for the plant extracts that are able to stimulate the regenerative ability of plants is a promising direction of biotechnological research.Materials and methods. Aseptic single-node cuttings of common buckwheat plantlets (varieties Dikul and Izumrud) were grown on MS nutrient media with plant extracts from Fagopyrum esculentum and Reynoutria japonica (0.1, 0.5, and 1%) for 21 days. The following morphobiological paramaters of the plantlets were evaluated: plant height, the number of internodes, the number of leaves, leaf length, and the number and length of roots.Results. Dealcoholized aqueous solutions of the extracts from F. esculentum and R. japonica in the studied concentrations (0.1-1%) significantly stimulated the growth and development of the buckwheat plantlets increasing their net reproduction rate (4.00-6.00) and rhizogenesis. The media with the plant extracts in concentrations of 0.1-0.5% were observed to produce the strongest positive effect. As the result, the morphobiological characteristics of the plantlets and the success rate of the micropropagation were the highest.

show abstract

Use of a Tissue Culture Method to Develop New Forms of Fagopyrum esculentum Moench

Cited by 2 publications

References 4 publications

Study of the Effect of Selective Media with High Doses of Zinc on Regeneration Ability and Rutin Accumulation in Common Buckwheat In Vitro

Study of the Effect of Selective Media with High Doses of Zinc on Regeneration Ability and Rutin Accumulation in Common Buckwheat In Vitro

Using plant extracts for the micropropagation of buckwheat

Contact Info

Product

Resources

About