Use of AFLP Markers for Gene Mapping and QTL Detection in the Rat

Otsen, M.; Bieman, M. Den; Kuiper, Martin; Pravenec, Michal; Křen, Vladimı́r; Kurtz, Theodore W.; Jacob, Howard J.; Lankhorst, A.; Zutphen, Bert F. M. Van

doi:10.1006/geno.1996.0562

Cited by 61 publications

(37 citation statements)

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“…This method has several advantages over other genomic fingerprint techniques and allowed us to perform multiple comparisons of DNA regions distributed across an entire genome, without the need for any prior knowledge of the sequence [97]. The genetic diversity revealed in this study is highly relevant to the recently reported AFLP characterization of inbred strains of mammals [98][99][100][101][102][103][104].…”

Section: Genetic Variability Of Gash:sal Hamstersmentioning

confidence: 85%

The genetic audiogenic seizure hamster from Salamanca: The GASH:Sal

Muñoz

Carballosa-Gautam

Yanowsky

et al. 2017

Epilepsy & Behavior

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The hamster has been previously described as a paroxysmal dystonia model, but our strain is currently recognized as a model of audiogenic seizures (AGS). The original first epileptic hamster appeared spontaneously at the University of Valladolid, where it was known as the GPG:Vall line, and was transferred to the University of Salamanca where a new strain was developed, named GASH:Sal. By testing auditory brainstem responses, the GASH:Sal exhibits elevated auditory thresholds that indicate a hearing impairment. Moreover, amplified fragment length polymorphism analysis distinguished genetic differences between the susceptible GASH:Sal hamster strain and the control Syrian hamsters. The GASH:Sal constitutes an experimental model of reflex epilepsy of audiogenic origin derived from an autosomal recessive disorder. Thus, the GASH:Sal exhibits generalized tonic-clonic seizures, characterized by a short latency period after auditory stimulation, followed by wild running, a convulsive phase, and finally stupor, with origin in the brainstem. The seizure profile of the GASH:Sal is similar to those exhibited by other models of inherited AGS susceptibility, which decreases after six months of age, but the proneness across generations is maintained. The GASH:Sal can be considered a reliable model of audiogenic seizures, suitable to investigate current antiepileptic pharmaceutical treatments as well as novel therapeutic drugs.This article is part of a Special Issue entitled Genetic Models-Epilepsy.

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Section: Genetic Variability Of Gash:sal Hamstersmentioning

confidence: 85%

The genetic audiogenic seizure hamster from Salamanca: The GASH:Sal

Muñoz

Carballosa-Gautam

Yanowsky

et al. 2017

Epilepsy & Behavior

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“…A BP quantitative trait locus (QTL) was identified within the differential chromosomal segment of SHR.1N in a set of recombinant inbred strains derived from the same progenitors (SHR and BN.Lx/Cub) as the congenic strain studied here. 29,43,44 In addition, the existence of the QTL was confirmed by generating a congenic strain. 29 A BP QTL within the same region of rat chromosome 20 was also identified in a cross involving New Zealand genetically hypertensive and BN rats.…”

Section: Discussionmentioning

confidence: 97%

Segment of Rat Chromosome 20 Regulates Diet-Induced Augmentations in Adiposity, Glucose Intolerance, and Blood Pressure

et al. 2003

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Abstract-Previous linkage and association studies have suggested that a region of human chromosome 6 containing the tumor necrosis factor (TNF)-␣ gene is involved in the pathogenesis of obesity and obesity-associated hypertension. The aim of the present investigation was to establish whether a segment of rat chromosome 20 (RNO20), which also contains the TNF-␣ gene, determines diet-induced changes in adiposity and blood pressure (BP). The results showed that a transfer of the RNO20 segment from the normotensive Brown Norway (BN) rat onto the background of the spontaneously hypertensive rat (SHR) is associated with a significantly greater increase in adiposity, glucose intolerance, circulating leptin levels, and BP during 12-week, high-fat-diet feeding. In contrast, the transfer is not associated with significant changes in these variables during 12-week, normal-diet feeding. In addition, sequencing of the TNF-␣ gene revealed differences between SHR and BN in the 5Ј-and 3Ј-regulatory regions of the gene. Subsequent analyses of TNF-␣ gene expression in fat, muscle, and liver, however, did not provide support for the functional involvement of these differences. In summary, the investigated RNO20 segment contains 1 or more gene variants that affect adiposity, glucose tolerance, serum leptin levels, and BP, but only when the animals are exposed to a particular environment, ie, high-fat-diet feeding. Further studies are needed to identify genes mediating these effects. Considering current changes in our lifestyle involving an increased calorie and fat intake, we believe that gene-environment interactions, such as those described here, play an important role in the current epidemic of obesity and obesityassociated hypertension.

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“…Although karyotyping has been shown to be a useful method for differentiating commercial strains of S. cerevisiae, it is of limited use for discriminating species with fewer chromosomes. The advantages of AFLP over other methods are also its reproducibility (Janssen et al, 1996;Jones et al, 1997) and its widespread application across all phyla (Janssen et al, 1996(Janssen et al, , 1997Folkertsma et al, 1996;Mueller et al, 1996;Otsen et al, 1996;Travis et al, 1996). Results described here indicate the value of AFLP in studying the intraspecific genetic relatedness of yeasts.…”

Section: Genetic Similarities Of Yeastsmentioning

confidence: 99%

“…AFLP is a technique that is based on the selective PCR amplification of restriction fragments from a total digest of DNA (Vos et al, 1995). The main use of AFLP to date has been as molecular markers, mostly for plant breeding programmes (Thomas et al, 1995) but also for mammalian species (Otsen et al, 1996). More recently, the effectiveness of AFLP for taxonomy and genetic diversity studies has been demonstrated in a number of biological systems including bacteria (Janssen et al, 1996(Janssen et al, , 1997, fungi (Mueller et al, 1996), plants (Travis et al, 1996) and animals (Folkertsma et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

AFLP fingerprinting for analysis of yeast genetic variation

Lopes

Rainieri

Henschke

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

106

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Amplified fragment length polymorphism (AFLP) was used to investigate genetic variation in commercial strains, type strains and winery isolates from a number of yeast species. AFLP was shown to be effective in discriminating closely related strains. Furthermore, sufficient similarity in the fingerprints produced by yeasts of a given species allowed classification of unknown isolates. The applicability of the method for determining genome similarities between yeasts was investigated by performing cluster analysis on the AFLP data. Results from two species, Sacchammyces cerevisiae and Dekkera btwxellensis, illustrate that AFLP is useful for the study of intraspecif ic genetic relatedness. The value of the technique in strain differentiation, species identification and the analysis of genetic similarity demonstrates the potential of AFLP in yeast ecology and evolutionary studies.

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Use of AFLP Markers for Gene Mapping and QTL Detection in the Rat

Cited by 61 publications

References 0 publications

The genetic audiogenic seizure hamster from Salamanca: The GASH:Sal

The genetic audiogenic seizure hamster from Salamanca: The GASH:Sal

Segment of Rat Chromosome 20 Regulates Diet-Induced Augmentations in Adiposity, Glucose Intolerance, and Blood Pressure

AFLP fingerprinting for analysis of yeast genetic variation

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