Use of agroindustrial wastes for the production of cellulases by Penicillium sp. FSDE15

Coêlho, Marina de Castro; Rocha, Juliene da Câmara; Santos, Francisco Naysson de Sousa; Gonçalves, Juan Carlos Ramos; Vasconcelos, Solange Maria de; Grisi, Teresa Cristina Soares de Lima; Santos, Sharline Florentino de Melo; Araújo, Demétrius Antônio Machado de; Carvalho-Gonçalves, Laís Campos Teixeira de

doi:10.1016/j.jksus.2021.101553

Cited by 12 publications

(4 citation statements)

References 21 publications

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“…An earlier study reported the agro-industrial waste hydrolysis capacity of Penicillium sp. FSDE15 cellulase of 1.02 U/ml, which is lower than our present study (de Castro Coelho et al, 2021). Another study on Trichoderma sp.…”

Section: Discussioncontrasting

confidence: 78%

Purification and characterization of cellulase produced by Novosphingobium sp. Cm1 and its waste hydrolysis efficiency and bio-stoning potential

Goswami

Boruah²,

Saikia

2022

Journal of Applied Microbiology

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The aim of the study was to purify and characterize cellulase from a previously isolated Novosphingobium sp. strain Cm1 and to evaluate its waste hydrolysis and bio-stoning efficiency. Materials and Methods:There is a growing demand for cellulase, a multipurpose enzyme widely used in industrial applications. Here, we purified cellulase from Novosphingobium sp. Cm1 by cellulose chromatography. SDS-PAGE revealed a molecular mass of 25 kDa. After 18-fold purification, the cellulase had an activity of 31.4 U/mg at pH of 5 and 40°C, and it retained activity at a wide range of pH and temperatures. The presence of Fe 2+ and Co 2+ boosted the enzyme activity by 57% and 25% respectively. The hydrolysing capacity of the strain towards cellulosic material was assessed for two paper types and the highest activity (2.6 ± 0.05 U/ml) was found with filter paper as the sole carbon source.Alterations in the structure of the papers as a result of bacterial hydrolysis were confirmed by scanning electron microscope and Fourier-transform infrared spectroscopy. The strain was also tested for its potential in various industrial applications and exhibited pectinolytic activity (6.78 ± 0.68 U/ml), xylanolytic activity (0.22 ± 0.14 U/ml) and bio-stoning ability. Conclusion:The highly active purified cellulase has a broad pH and temperature range. The strain possesses waste-hydrolysing ability, pectinolytic and xylanolytic ability along with bio-stoning capacity. Significance and Impact of the Study:The efficacy and versatility of the enzyme from Novosphingobium sp. Cm1 make it an excellent candidate for diverse industrial applications.

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Section: Discussioncontrasting

confidence: 78%

Purification and characterization of cellulase produced by Novosphingobium sp. Cm1 and its waste hydrolysis efficiency and bio-stoning potential

Goswami

Boruah²,

Saikia

2022

Journal of Applied Microbiology

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“…Straw, logs, bark, vegetable pulp, legumes, and many others are by-products of industrial processes that do not have added value, as these residues are often not used directly [119]. However, sugars, proteins, fibers, and minerals are compounds highly present in the composition of these by-products, thus providing alternative sources of carbohydrates and nitrogen to the detriment of synthesizing sources of these nutrients applied in bioprocesses [120].…”

Section: Agroindustrial Wastementioning

confidence: 99%

“…Numerous bioprocesses have been designed to make it possible to use these materials as substrates in synthesizing multiple molecules with high-added value, such as microbial proteins, organic acids, ethanol, and enzymes [121]. Using agro-industrial by-products as a substrate for bioprocesses not only makes economic sense but also helps solve the environmental problems arising from accumulating these materials in the environment [120].…”

Section: Agroindustrial Wastementioning

confidence: 99%

Research Progress and Trends on Utilization of Lignocellulosic Residues as Supports for Enzyme Immobilization via Advanced Bibliometric Analysis

et al. 2023

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Lignocellulosic biomasses are used in several applications, such as energy production, materials, and biofuels. These applications result in increased consumption and waste generation of these materials. However, alternative uses are being developed to solve the problem of waste generated in the industry. Thus, research is carried out to ensure the use of these biomasses as enzymatic support. These surveys can be accompanied using the advanced bibliometric analysis tool that can help determine the biomasses used and other perspectives on the subject. With this, the present work aims to carry out an advanced bibliometric analysis approaching the main studies related to the use of lignocellulosic biomass as an enzymatic support. This study will be carried out by highlighting the main countries/regions that carry out productions, research areas that involve the theme, and future trends in these areas. It was observed that there is a cooperation between China, USA, and India, where China holds 28.07% of publications in this area, being the country with the greatest impact in the area. Finally, it is possible to define that the use of these new supports is a trend in the field of biotechnology.

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“…Recently, our research group reported that Penicillium spp. FSDE15 strain showed overproduction of cellulases in Castro Coêlho et al [14], but the potential to obtain xylanases has not been adequately investigated.…”

Section: Introductionmentioning

confidence: 99%

Production of xylanases from Penicillium sp. FSDE15 using agro-industrial residues and its biotechnological potential

Nunes,

Padilha,

Costa

et al. 2024

Preprint

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This study investigates the production and application of xylanases derived from the fungus Penicillium sp. FSDE15, obtained via solid-state fermentation (SSF). Wheat bran served as the substrate, either in its pure form or mixed with sisal fiber. Penicillium sp. underwent incubation in SSF for 168 h, with daily sampling to evaluate xylanase activity. Xylanase analyses were conducted under various temperature and pH conditions to optimize enzymatic activity, with the optimal conditions determined as 50°C and pH 4.8. SDS-PAGE electrophoresis revealed the enzyme's molecular weight as 66 kDa. Evaluation of Penicillium sp. xylanase for enzymatic hydrolysis of sugarcane straw (untreated and alkaline pretreated) and corn cob xylan showcased significant activity, with 102.34 U/g and a productivity of 1.67 U/g.h observed in 72 h, surpassing experiments with the blend of bran and sisal fiber. Electrophoresis analysis identified a single protein band at 66 kDa. Although optimal conditions were identified, the enzyme exhibited instability at alkaline pH values (> 6.0) and elevated temperatures (> 60°C). When compared to a commercial enzyme, FSDE15 demonstrated satisfactory performance in saccharification and xylooligosaccharide (XOS) production, including X2, indicating its biotechnological potential. This study underscores the efficacy of SSF in xylanase production by Penicillium sp. FSDE15 has implications for converting lignocellulosic biomass into value-added products, particularly in saccharification and XOS production.

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Use of agroindustrial wastes for the production of cellulases by Penicillium sp. FSDE15

Cited by 12 publications

References 21 publications

Purification and characterization of cellulase produced by Novosphingobium sp. Cm1 and its waste hydrolysis efficiency and bio-stoning potential

Purification and characterization of cellulase produced by Novosphingobium sp. Cm1 and its waste hydrolysis efficiency and bio-stoning potential

Research Progress and Trends on Utilization of Lignocellulosic Residues as Supports for Enzyme Immobilization via Advanced Bibliometric Analysis

Production of xylanases from Penicillium sp. FSDE15 using agro-industrial residues and its biotechnological potential

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