Use of Amplified-Fragment Length Polymorphism To Study the Ecology of Campylobacter jejuni in Environmental Water and To Predict Multilocus Sequence Typing Clonal Complexes

Lévesque, Simon; St-Pierre, Karen; Frost, Éric; Arbeit, Robert D.; Michaud, Sophie

doi:10.1128/aem.06527-11

Cited by 8 publications

(5 citation statements)

References 31 publications

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“…There was a clear overlap of swine and environmental STs, although we cannot ascertain the direction in which C. coli was transmitted. Additionally, the diversity of STs present in the environment at farm and slaughter, including unique STs, suggest the environment plays a role in increasing the genetic diversity, as has been previously reported [36] .…”

Section: Discussionsupporting

confidence: 61%

Phylogenetic Analysis Reveals Common Antimicrobial Resistant Campylobacter coli Population in Antimicrobial-Free (ABF) and Commercial Swine Systems

Quintana-Hayashi

Thakur

2012

PLoS ONE

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The objective of this study was to compare the population biology of antimicrobial resistant (AR) Campylobacter coli isolated from swine reared in the conventional and antimicrobial-free (ABF) swine production systems at farm, slaughter and environment. A total of 200 C. coli isolates selected from fecal, environmental, and carcass samples of ABF (n = 100) and conventional (n = 100) swine production systems were typed by multilocus sequence typing (MLST). Sequence data from seven housekeeping genes was analyzed for the identification of allelic profiles, sequence types (STs) and clonal complex determination. Phylogenetic trees were generated to establish the relationships between the genotyped isolates. A total of 51 STs were detected including two novel alleles (glnA 424 and glyA 464) and 14 novel STs reported for the first time. The majority of the C. coli isolates belonged to ST-854 (ABF: 31, conventional: 17), and were grouped in clonal complex ST-828 (ABF: 68%, conventional: 66%). The mean genetic diversity (H) for the ABF (0.3963+/−0.0806) and conventional (0.4655+/−0.0714) systems were similar. The index of association () for the ABF ( = 0.1513) and conventional ( = 0.0991) C. coli populations were close to linkage equilibrium, indicative of a freely recombining population. Identical STs were detected between the pigs and their environment both at farm and slaughter. A minimum spanning tree revealed the close clustering of C. coli STs that originated from swine and carcass with those from the environment. In conclusion, our study reveals a genotypic diverse C. coli population that shares a common ancestry in the conventional and ABF swine production systems. This could potentially explain the high prevalence of antimicrobial resistant C. coli in the ABF system in the absence of antimicrobial selection pressure.

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Section: Discussionsupporting

confidence: 61%

Phylogenetic Analysis Reveals Common Antimicrobial Resistant Campylobacter coli Population in Antimicrobial-Free (ABF) and Commercial Swine Systems

Quintana-Hayashi

Thakur

2012

PLoS ONE

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“…5 However, Campylobacter is also isolated from a wide range of domestic animals and wildlife 5,6 and even from environmental samples, such as soil, compost, and water. 7,8 This suggests that Campylobacter may possess unique survival mechanisms to sustain its viability under harsh environmental conditions.…”

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confidence: 99%

Stimulation of biofilm formation by oxidative stress inCampylobacter jejuniunder aerobic conditions

Kim

Jeon

2016

Virulence

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“…The method shows high discriminatory power and good reproducibility and has proved to be efficient for discriminating at the species level and below in various taxa (Janssen et al, 1996, Savelkoul et al, 1999. The development and application of AFLP as a fingerprinting method has led to significant progress in the study of the genetic diversity and taxonomy of bacteria (Cappello et al, 2008;Hamza et al, 2012;Lazzi et al, 2009;Lévesque et al, 2012;Nabhan et al, 2012). However, only a few studies have been published using AFLP to investigate the phylogeny of Photobacterium (Ast et al, 2007), most of them dealing with P. damselae…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Genetic diversity analysis of isolates belonging to the Photobacterium phosphoreum species group collected from salmon products using AFLP fingerprinting

Jérôme¹,

Macé

Dousset

et al. 2016

International Journal of Food Microbiology

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An accurate amplified fragment length polymorphism (AFLP) method, including three primer sets for the selective amplification step, was developed to display the phylogenetic position of Photobacterium isolates collected from salmon products. This method was efficient for discriminating the three species Photobacterium phosphoreum, Photobacterium iliopiscarium and Photobacterium kishitanii, until now indistinctly gathered in the P. phosphoreum species group known to be strongly responsible for seafood spoilage. The AFLP fingerprints enabled the isolates to be separated into two main clusters that, according to the type strains, were assigned to the two species P. phosphoreum and P. iliopiscarium. P. kishitanii was not found in the collection. The accuracy of the method was validated by using gyrB-gene sequencing and luxA-gene PCR amplification, which confirmed the species delineation. Most of the isolates of each species were clonally distinct and even those that were isolated from the same source showed some diversity. Moreover, this AFLP method may be an excellent tool for genotyping isolates in bacterial communities and for clarifying our knowledge of the role of the different members of the Photobacterium species group in seafood spoilage.

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Use of Amplified-Fragment Length Polymorphism To Study the Ecology of Campylobacter jejuni in Environmental Water and To Predict Multilocus Sequence Typing Clonal Complexes

Cited by 8 publications

References 31 publications

Phylogenetic Analysis Reveals Common Antimicrobial Resistant Campylobacter coli Population in Antimicrobial-Free (ABF) and Commercial Swine Systems

Phylogenetic Analysis Reveals Common Antimicrobial Resistant Campylobacter coli Population in Antimicrobial-Free (ABF) and Commercial Swine Systems

Stimulation of biofilm formation by oxidative stress inCampylobacter jejuniunder aerobic conditions

Genetic diversity analysis of isolates belonging to the Photobacterium phosphoreum species group collected from salmon products using AFLP fingerprinting

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