Use of an anionic collagen matrix made from bovine intestinal serosa for in vivo repair of cranial defects

Abstract

Polymeric biomaterials composed of extracellular matrix components possess osteoconductive capacity that is essential for bone healing. The presence of collagen and the ability to undergo physicochemical modifications render these materials a suitable alternative in bone regenerative therapies. The objective of this study was to evaluate the osteogenic capacity of collagen-based matrices (native and anionic after alkaline hydrolysis) made from bovine intestinal serosa (MBIS). Twenty-five animals underwent surgery to create a cranial defect to be filled with native and anionic collagen matrixes, mmineralized and non mineralized. The animals were killed painlessly 6 weeks after surgery and samples of the wound area were submitted to routine histology and morphometric analysis. In the surgical area there was new bone formation projecting from the margins to the center of the defect. More marked bone neoformation occurred in the anionic matrices groups in such a way that permitted union of the opposite margins of the bone defect. The newly formed bone matrix exhibited good optical density of type I collagen fibers. Immunoexpression of osteocalcin by osteocytes was observed in the newly formed bone. Morphometric analysis showed a greater bone volume in the groups receiving the anionic matrices compared to the native membranes. Mineralization of the biomaterial did not increase its osteoregenerative capacity. In conclusion, the anionic matrix exhibits osteoregenerative capacity and is suitable for bone reconstruction therapies.

In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

“…Microtomographically, the presence of elastin and collagen matrices was not detected in any of the defects treated (E24/37 and C24/25), probably due to the process of biodegradation with a concomitant rate of bone neoformation, being an essential factor as it promotes cell proliferation and, at the same time, allows space for bone growth [ 26 ]. Centripetal formation was still be observed, more evident on C24/25, which showed a greater deposition of partially mineralized bone, a fact associated with the porous intestinal serosa membrane, abundant in collagen, and a three-dimensional biomaterial with cellular affinity and elasticity, which allows the creation of a microenvironment facilitating cell growth [ 11 , 19 , 27 , 28 ].…”

“…The manufacturing process of elastin and collagen matrices and their characterizations have been described in greater detail in previously published studies [ 7 , 19 , 27 , 40 , 41 , 42 ]. The elastin matrices used were prepared by the Biochemistry and Biomaterials Group of the São Carlos Institute of Chemistry (University of São Paulo—USP, Brazil) by adapting the methodology used to obtain collagen [ 7 , 8 , 14 ].…”

Construction of Dual-Biofunctionalized Chitosan/Collagen Scaffolds for Simultaneous Neovascularization and Nerve Regeneration

“…These biofunctional scaffolds enhanced SC and EC proliferation rates, the expression levels of angiogenic and neuroregeneration-related genes and proteins by these cells, and neovascularization, axonal repair, myelination, and neuromuscular transmission in vivo compared to bare CC scaffolds and biofunctionalized scaffolds incorporating only IKVAV or VEGF. Chitosan is a highly cationic polysaccharide [ 37 ], while collagen mainly carries anionic groups [ 38 ], thus facilitating strong CC complex formation via electrostatic interactions. After confirming this complex formation by UV spectroscopy, dual-biofunctionalized chitosan/collagen composite scaffolds were constructed by immobilizing IKVAV and VEGF via heparin as confirmed by various quantitative and qualitative analyses including FTIR, TBO staining, ELISA, and fluorescence staining.…”

Biomateriais sintéticos e xenógenos com alto potencial clínico para reparo tecidual

“…Também Pettian et al produziram uma membrana de colagénio a partir da camada serosa de intestino bovino e avaliaram a sua capacidade de reparação óssea in vivo, num modelo de ratos com defeito ósseo craniano. Apartir de análise morfométrica, os autores verificaram um aumento significativo de volume ósseo nos grupos de teste (Pettian, M. S. et al, 2018), resultados em conformidade com aqueles obtidos por Hirata et al num estudo semelhante, em que analisaram membranas da serosa intestinal e membranas de pericárdio de bovino (Hirata, H. H. et al, 2015).…”