Use of an Inhibition Enzyme-Linked Immunosorbent Assay for Quantification of Capsular Polysaccharide or Proteins in Vaccines

Abstract

An inhibition enzyme-linked immunosorbent assay (ELISA) is described for quantification of capsular polysaccharide or proteins in vaccines and other samples containing whole cells or extracts of Actinobacillus pleuropneumoniae. The assay can be used to quantify any antigen that can be purified and for which highly specific antibodies are not available. The assay can be carried out by any laboratory capable of performing an ELISA.

Identification and characterization of three novel EsaI/EsaR quorum‐sensing controlled stewartan exopolysaccharide biosynthetic genes in Pantoea stewartii ssp. stewartii

“…Stewartan was quantified in solution using an Inhibition Enzyme‐Linked Immunosorbent Assay (Inzana and Champion, 2007). Biotin‐LC‐hydrazide (Pierce Chemical, Rockford, IL) was coupled directly to the carboxyl groups of glucuronic acid of purified stewartan with stoichiometric amounts of ethyl‐dimethylaminopropyl‐carbodiimide HCl (EDC, Pierce Pierce Chemical, Rockford, IL) according to manufacturer's instructions.…”

Adhesion Protein ApfA of Actinobacillus pleuropneumoniae Is Required for Pathogenesis and Is a Potential Target for Vaccine Development

“…It colonizes the epithelial cells of the lower respiratory tract of pigs (1,2). The infected animals develop disease symptoms such as chronic necrotizing pneumonia, acute fibrinous pneumonia, and pleuritis with high mortality, which leads to large economic losses in the pig farming industry worldwide (3).…”

“…A total of 15 serotypes of A. pleuropneumoniae have been identified on the basis of their capsular and lipopolysaccharide (LPS) antigens (3). Although their virulence and regional prevalence vary, all the serotypes cause disease, and with the growing emergence of antibiotic resistance and the rising concern about food safety, vaccination to prevent A. pleuropneumoniae infections is of increasing relevance (2,4).…”

Draft Genome Sequences of Six Actinobacillus pleuropneumoniae Serotype 8 Brazilian Clinical Isolates: Insight into New Applications

“…There are 15 known serotypes of this microorganism, the prevalence of which varies greatly worldwide (2). Serotype 8 isolates, the most widespread in southeastern Brazil (3), display high cytotoxic activity and are generally responsible for low mortality but high morbidity.…”

“… Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, a costly, severe, and highly contagious infectious disease ( 1 ). There are 15 known serotypes of this microorganism, the prevalence of which varies greatly worldwide ( 2 ). Serotype 8 isolates, the most widespread in southeastern Brazil ( 3 ), display high cytotoxic activity and are generally responsible for low mortality but high morbidity.…”