Use of antagonistic bacilli for biocontrol of fungi degrading fresh wood

Мелентьев, А. И.; Helistö, Pirkko; Kuzmina, L. Yu.; Galimzyanova, N. F.; Актуганов, Г. Э.; Korpela, Timo

doi:10.1134/s0003683806010091

Cited by 17 publications

(11 citation statements)

References 16 publications

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“…Recently, mycolytic enzyme secreting Bacillus sp. was reportedly explored as biocontrol agent against fresh wood decaying (rotting) fungi that causes soft rot [12]. Chitinolytic Streptomyces strains are the most suitable choice as potential biological control agents [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of an extracellular chitinase from antagonistic Streptomyces violaceusniger

Nagpure

Gupta

2012

J. Basic Microbiol

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The actinomycetes Streptomyces violaceusniger showed strong antagonistic activity against various tested wood rotting fungi. An extracellular chitinase, produced by antagonistic S. violaceusniger MTCC 3959, was purified as follows: ammonium sulfate precipitation, chitin affinity and chromatographic separation of Q Sepharose. The molecular mass of the purified chitinase was estimated as 56.5 kDa by SDS-PAGE. Chitinase was optimally active at pH of 5.0 and 50 °C. It retained almost 100% activity at pH 5.0 and also had high thermal tolerance at 50 °C. Enzyme activity was inhibited by Hg(2+) and Ag(+) cations, but was neither substantially inhibited by K(+) cation nor by chelating agent EDTA. The apparent Km and Vmax at 37 °C were 0.1426 mM and 6.6 U/mg, respectively using pNP-(GlcNAc)2 as substrate. The 56.5 kDa chitinase of strain MTCC 3959 represented an exo-type activity. The purified chitinase was further identified by MALDI-TOF. The results of peptide mass fingerprinting showed that 10 tryptic peptides of the chitinase were identical to the chitinase C from Streptomyces albus J1074 (GenBank Accession No. gi|239982330). The sequence of N-terminal amino acid (AA) of the chitinase was determined to be G-D-G-T-G-P-G-P-G-P.

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Section: Introductionmentioning

confidence: 99%

Purification and characterization of an extracellular chitinase from antagonistic Streptomyces violaceusniger

Nagpure

Gupta

2012

J. Basic Microbiol

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“…Moreover, extensive use of synthetic compounds to improve crop productivity and disease control is a growing concern. At present, chemical fungicides such as Thiram, Bavistin and Benomyl are generally used to control Fusarium wilt (Vidyasekaran et al, 1997;Meena et al, 2002;Melent'ev et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Biological control ofFusarium wilt of pigeonpea byPantoea dispersa, a field assessment

et al. 2008

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Fusarium wilt is one of the major yield and growth-limiting factors of pigeonpea (Cajanus cajan). For an ecofriendly and sustainable management of such a disease, a novel mycolytic strain Pantoea dispersa was evaluated against fungal pathogen Fusarium udum that is known to be infecting the susceptible variety of pigeonpea (T-15-15), commonly prevalent in India. To study the efficacy of P. dispersa as a biocontrol agent in comparison with chemical fungicide Bavistin and antifungal biocontrol agent Trichoderma Monitor WP was studied in both pot and field experiments. In vitro, P. dispersa inhibits the growth of F. udum. In the pot experiment, P. dispersa the treated pigeon pea (T-15-15) seeds showed higher percentage of seed germination and decreased wilt incidence as compared to chemical fungicide; Bavistin and antifungal biocontrol agent Trichoderma Monitor WP treatments. Moreover, the root, shoot lengths and growth were also found to be higher. Due to high impact of P. dispersa in in vitro condition on controlling the F. udum growth, further study was conducted in field in a wilt-stricken plot. These studies were repeated for three cropping seasons (2004/2007). The seed dressing by P. dispersa reduced wilt incidence (47%) during field trials, which is greater than Bavistin (41%) and Trichoderma Monitor WP (36%) treatments. Hence P. dispersa was proven superior to Trichoderma Monitor WP and Bavistin; which are used as commercial Fusarium wilt control agents.

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“…Many authors have already been reported the isolation, purification, and characterization of lipopeptides against the plant pathogenic fungal groups (Brown and Bruce, 1999;Moyne et al, 2001;Feio et al, 2004;Melent'ev et al, 2006). The structures of lipopeptides were determined and the possible mechanism between the antagonistic activity of lipopeptides and fungal samples were also discussed earlier (Melent'ev et al, 2006;Romero et al, 2007;Volpon et al, 2007).…”

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confidence: 99%

“…The structures of lipopeptides were determined and the possible mechanism between the antagonistic activity of lipopeptides and fungal samples were also discussed earlier (Melent'ev et al, 2006;Romero et al, 2007;Volpon et al, 2007). However, evidence for antimicrobial and antifungal activity of Bacillus sp.…”

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confidence: 99%

Evaluation of antagonistic activities of Bacillus subtilis and Bacillus licheniformis against wood-staining fungi: In vitro and in vivo experiments

et al. 2009

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The antifungal activity of bacterial strains Bacillus subtilis EF 617317 and B. licheniformis EF 617325 was demonstrated against sapstaining fungal cultures Ophiostoma flexuosum, O. tetropii, O. polonicum, and O. ips in both in vitro and in vivo conditions. The crude active supernatant fractions of 7 days old B. subtilis and B. licheniformis cultures inhibited the growth of sapstaining fungi in laboratory experiments. Thermostability and pH stability of crude supernatants were determined by series of experiments. FT-IR analysis was performed to confirm the surface structural groups of lipoproteins present in the crude active supernatant. Partial purification of lipopeptides present in the crude supernatant was done by using Cellulose anion exchange chromatography and followed by Sephadex gel filtration chromatography. Partially purified compounds significantly inhibited the sapstaining fungal growth by in vitro analysis. The lipopeptides responsible for antifungal activity were identified by electrospray ionization mass spectrometry after partial purification by ion exchange and gel filtration chromatography. Four major ion peaks were identified as m/z 1023, 1038, 1060, and 1081 in B. licheniformis and 3 major ion peaks were identified as m/z 1036, 1058, and 1090 in B. subtilis. In conclusion, the partially purified lipopeptides may belong to surfactin and iturin family. In vivo analysis for antifungal activity of lipopeptides on wood was conducted in laboratory. In addition, the potential of extracts for fungal inhibition on surface and internal part of wood samples were analyzed by scanning electron microscopy.

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Use of antagonistic bacilli for biocontrol of fungi degrading fresh wood

Cited by 17 publications

References 16 publications

Purification and characterization of an extracellular chitinase from antagonistic Streptomyces violaceusniger

Purification and characterization of an extracellular chitinase from antagonistic Streptomyces violaceusniger

Biological control ofFusarium wilt of pigeonpea byPantoea dispersa, a field assessment

Evaluation of antagonistic activities of Bacillus subtilis and Bacillus licheniformis against wood-staining fungi: In vitro and in vivo experiments

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