Use of Autologous Leucocyte- and Platelet-Rich Plasma (L-PRP) in the Treatment of Aural Hematoma in Dogs

Perego, Roberta; Spada, Eva; E, Moneta; Baggiani, Luciana; Proverbio, Daniela

doi:10.3390/vetsci8090172

Cited by 8 publications

(8 citation statements)

References 56 publications

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“…The overall (both groups) median and mean LOAD and CBPI scores, and QOL obtained from the questionnaires are summarized in Table 1. When the SXA group was analyzed separately from the PRP group the data was the following: SXA median LOAD was 11 (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19), CBPI 3 (0-13), and QOL good (3). The mean LOAD was 13 (SD 9), CBPI was 13 (SD 23), and QOL was good (3, SD 1) (Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…The mean LOAD was 13 (SD 9), CBPI was 13 (SD 23), and QOL was good (3, SD 1) (Table 2). For the PRP group, the median LOAD was 11 (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19), CBPI 3 (0-13), and QOL good (3). The mean LOAD was 13 (SD 9), CBPI was 13 (SD 23), and QOL was good (3, SD 1) (Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…In veterinary medicine, platelet-rich plasma has been used and reported as a treatment option for a variety of disease processes with favorable outcomes and no major complications ( 11 – 19 ). Platelet-rich plasma has been described as a treatment for supraspinatus tendinopathy ( 11 ), early partial cranial cruciate ligament tears ( 12 ), bilateral hip osteoarthritis ( 13 ), large cutaneous wounds ( 14 ), aural hematomas ( 15 ), lumbosacral stenosis ( 16 ), stifle osteoarthritis secondary to medically managed chronic cranial cruciate ligament tears ( 17 ), and for bone healing in traumatic fractures ( 18 ) and also high-tibial osteotomy ( 19 ). In these cases, all had favorable outcomes, except for use in high-tibial osteotomies in which there was no significant difference between control and experimental groups.…”

Section: Introductionmentioning

confidence: 99%

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Owner assessed outcomes following elbow arthroscopy with or without platelet rich plasma for fragmented medial coronoid process

Cruz¹,

Mason

2022

Front. Vet. Sci.

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ObjectiveDocument the outcomes of bilateral arthroscopic subtotal coronoidectomy for the fragmented medial coronoid process, quantify persistent lameness that required additional treatment (PRP), and document the outcomes of dogs that followed up with PRP injections.Study designRetrospective study.Sample populationOverall, 115 dogs underwent arthroscopy alone and 31 received PRP at least 6 weeks after arthroscopy. The owner's response rate was ~50% (73 dogs).MethodsCollected data included signalment, unilateral or bilateral clinical signs, intra-articular chondroprotective injection during the procedure, if PRP intra-articular injection was received postoperatively, and if it was received, the time from the initial surgery to administration was recorded. Outcomes were assessed via standardized owner questionnaires using the Liverpool Osteoarthritis in Dogs (LOAD) score, the Canine Brief Pain Inventory (CBPI) score, and the overall quality of life (QOL) assessment.ResultsApproximately 20% of the patients received PRP post-operatively due to persistent lameness following surgery. Similar pain scores were found between the two groups with an average of 11–13 LOAD score, 13–15 CBPI score, and good quality of life. Older animals at the time of surgery and those that received pain-relieving medications after the procedure were more painful and affected their functional outcome. PRP as an adjunctive therapy achieved a perceived good to excellent quality of life in ~90% of pets in this population.ConclusionArthroscopy and subtotal coronoidectomy followed by PRP, if needed, seemed to decrease pain, and improve lameness in the long term.Clinical significancePRP should be considered as adjunctive therapy in dogs with the limited response to arthroscopy alone.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Owner assessed outcomes following elbow arthroscopy with or without platelet rich plasma for fragmented medial coronoid process

Cruz¹,

Mason

2022

Front. Vet. Sci.

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“…Among the orthobiologic blood products, particularly platelet products are already frequently used in clinical practice. In canine medicine, PRP or platelet concentrates have been used to treat several conditions, including osteoarthritis [ 17 , 18 , 19 , 20 ], lumbosacral stenosis [ 21 ], wounds [ 22 , 23 ], corneal ulcera [ 24 ], and aural hematoma [ 25 ]. While clinical benefits were shown, a major disadvantage of platelet concentrate is its limited long-term storage, as it cannot be frozen [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same

Hagen

Holland²,

Brandt³

et al. 2022

Animals

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Platelet lysate (PL) is an attractive platelet-based therapeutic tool and has shown promise as xeno-free replacement for fetal bovine serum (FBS) in human and equine mesenchymal stromal cell (MSC) culture. Here, we established a scalable buffy-coat-based protocol for canine PL (cPL) production (n = 12). The cPL was tested in canine adipose MSC (n = 5) culture compared to FBS. For further comparison, equine adipose MSC (n = 5) were cultured with analogous equine PL (ePL) or FBS. During canine blood processing, platelet and transforming growth factor-β1 concentrations increased (p < 0.05 and p < 0.001), while white blood cell concentrations decreased (p < 0.05). However, while equine MSC showed good results when cultured with 10% ePL, canine MSC cultured with 2.5% or 10% cPL changed their morphology and showed decreased metabolic activity (p < 0.05). Apoptosis and necrosis in canine MSC were increased with 2.5% cPL (p < 0.05). Surprisingly, passage 5 canine MSC showed less genetic aberrations after culture with 10% cPL than with FBS. Our data reveal that using analogous canine and equine biologicals does not entail the same results. The buffy-coat-based cPL was not adequate for canine MSC culture, but may still be useful for therapeutic applications.

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“…In contrast, studies that support the use of L-PRP due to the presence of leucocytes in the inflammatory phase of wound healing were also published [ 76 , 78 , 79 , 80 ]. In veterinary medicine, L-PRP has been used in 17 dogs with aural hematomas that were resolved completely after the injection of L-PRP [ 81 ]. In our study, a commercially available system was used (Tropocells) to prepare PRP which had a very low number of WBCs, no RBCs, and a high number of monocytes.…”

Section: Discussionmentioning

confidence: 99%

Locally Injected Autologous Platelet-Rich Plasma Improves Cutaneous Wound Healing in Cats

Angelou

Psalla

Dovas

et al. 2022

Animals

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Cutaneous defects in cats are commonly encountered in clinical practice, and healing can be accomplished by first or second intention. Platelet-rich plasma (PRP) is characterized by a plasma concentration containing a large number of platelets in a small volume of plasma. The objective of the present study was to record the efficacy of PRP infiltration in open wounds in laboratory cats. Six wounds were created in the dorsal midline of eight laboratory cats, with the wounds of one side designated as the PRP group and the wounds of the other side as the control group. Wound healing was evaluated by daily clinical examination, planimetry, laser Doppler flowmetry, and histologic examination on days 0, 7, 14, and 25, and by measurement of metalloproteinases (MMPs)-2 and -9 and tissue inhibitor metalloproteinase (TIMP)-1 on days 0, 14, and 25. Based on the results of the present study, the mean time for full coverage with granulation tissue was shorter in the PRP group, the mean contraction and total wound healing percentage were increased compared to the control group, and finally, the perfusion measured with laser Doppler flowmetry was higher in the PRP group during all examination days. In conclusion, this is the first study focusing on the topical application of PRP in the treatment of open wounds in laboratory cats, and our results are encouraging—showing a more rapid healing in the PRP group.

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Use of Autologous Leucocyte- and Platelet-Rich Plasma (L-PRP) in the Treatment of Aural Hematoma in Dogs

Cited by 8 publications

References 56 publications

Owner assessed outcomes following elbow arthroscopy with or without platelet rich plasma for fragmented medial coronoid process

Owner assessed outcomes following elbow arthroscopy with or without platelet rich plasma for fragmented medial coronoid process

Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same

Locally Injected Autologous Platelet-Rich Plasma Improves Cutaneous Wound Healing in Cats

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