Use of BHK cell culture-adapted Newcastle disease virus for immunization of chicks

Reddy, G. S.; Srinivasan, V.A.

doi:10.1016/0264-410x(92)90006-6

Cited by 10 publications

(5 citation statements)

References 4 publications

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“…However, the 15 th and 18 th passage viruses did not produce any specific symptoms. These results confirm that the Komarov vaccine causes respiratory symptoms in unprimed chicks, which correlates with the results of previous studies ( Reddy and Srinivasan, 1992 ; OIE, 2008 )…”

Section: Discussionsupporting

confidence: 92%

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Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov

Visnuvinayagam¹,

Thangavel²,

Lalitha³

et al. 2015

Braz. J. Microbiol.

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Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccine virulence for safe vaccination by adapting the virus in a chicken embryo fibroblast cell culture (CEFCC) system. Eighteen passages were carried out by CEFCC, and the pathogenicity was assessed on the basis of the mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index, at equal passage intervals. Although the reduction in virulence demonstrated with increasing passage levels in CEFCC was encouraging, 20% of the 2-week-old birds showed paralytic symptoms with the virus vaccine from the 18th(final) passage. Thus, a tissue-culture-adapted vaccine would demand a few more passages by CEFCC in order to achieve a complete reduction in virulence for use as a safe and effective vaccine, especially among younger chicks. Moreover, it can be safely administered even to unprimed 8-week-old birds.

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Section: Discussionsupporting

confidence: 92%

“…have been employed only among 8-week-old chicks that had already been primed at 2 weeks of age with a lentogenic vaccine such as strains LaSota, F, etc. ( Reddy and Srinivasan, 1992 ; OIE, 2008 ). Otherwise, the mesogenic vaccine causes mortality in unprimed chicks, even at the age of 8 weeks.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov

Visnuvinayagam¹,

Thangavel²,

Lalitha³

et al. 2015

Braz. J. Microbiol.

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“…These findings concurred with earlier results (20,21). However, in a few instances, an elevated ICPI following cell culture passage of lentogenic and mesogenic strains was noticed, for which no hypothesis has been proposed (32,36). The MDT and ICPI values (120 hr and 0.1) of the 50th passage virus indicate that the virus has become lentogenic biologically.…”

Section: Discussionmentioning

confidence: 99%

Molecular Changes of the Fusion Protein Gene of Chicken Embryo Fibroblast–Adapted Velogenic Newcastle Disease Virus: Effect on Its Pathogenicity

Mohan

Dey

Kumanan³

2005

Avian Diseases

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Molecular changes of cell culture-adapted Newcastle disease virus (NDV) were studied by adapting a velogenic NDV isolated from commercial layer chicken-to-chicken embryo fibroblast (CEF) cells. The isolate was passaged 50 times in CEF cells. At every 10th passage the virus was characterized conventionally by mean death time analysis, intracerebral pathogenicity index, and virus titration. As the passage level increased, a gradual reduction in the virulence of the virus was observed. Molecular characterization of the virus included cloning and sequencing of a portion of the fusion gene (1349 bp) encompassing the fusion protein cleavage site (FPCS), which was previously amplified by reverse transcription-polymerase chain reaction. Sequence analysis revealed a total of 134 nucleotide substitutions, which resulted in the change of 41 amino acids between the parent and the 50th passage virus. Pathogenicity studies conducted in 20-wk-old seronegative chickens revealed gross and histopathologic changes in the chickens injected with the parent virus and absence of the lesions in chickens injected with the adapted virus. The 50th passage cell culture virus was back-passaged five times in susceptible chickens and was subjected to virulence attribute analysis and sequence analysis of the FPCS region, with minor differences between them.

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“…Previous studies have also shown that co-expression of the both F and HN genes more effectively induces immune response to NDV [ 5 10 ]. However, the result of a previous study indicated that a bivalent pIRES plasmid containing both HN and F genes of NDV induced lower IFN-γ and cell-mediated immunity (CMI) responses than constructs containing single genes in maternal antibody-positive commercial chickens [ 18 ]. Both CMI and humoral response are thought to play a primary role against NDV infection in chickens [ 20 21 ], although a recent study indicated that protection efficacy of a NDV DNA vaccine is more closely related to humoral immune response than CMI [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes

et al. 2016

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The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant difference in enzyme-linked immunosorbent assay antibody levels (p < 0.05) elicited by either pIRES/F, pIRES/F+ pIRES/HN or pIRES-F/HN at one week after the booster in specific pathogen free chickens when compared with the inactivated vaccine alone. Taken together, these results indicated that recombinant pDNA could be used to increase the efficacy of the inactivated vaccine immunization procedure.

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Use of BHK cell culture-adapted Newcastle disease virus for immunization of chicks

Cited by 10 publications

References 4 publications

Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov

Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov

Molecular Changes of the Fusion Protein Gene of Chicken Embryo Fibroblast–Adapted Velogenic Newcastle Disease Virus: Effect on Its Pathogenicity

Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes

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