Use of biofungicides to address conventional Botrytis blight control challenges in wild blueberry production

Percival, David; Abbey, Joel; Lu, Hongbao; Harris, Lora

doi:10.17660/actahortic.2017.1180.32

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…With Botrytis management products accounting for more than 60% of the fungicide costs and concerns over detectable residues by consumers, there is a need to develop disease management approaches that will deliver no detectable levels of fungicide residues in processed fruit. This has given rise to the interest in biofungicides including Serifel TM (Bacillus amyloliquefaciens), Serenade Max ® (Bacillus subtilis) and Fracture™ (BLAD polypeptide) (Percival et al, 2016). Previous studies have reported that some biological control agents, plant extracts and biologically active natural products can serve as excellent alternative to conventional fungicides presently being used (Shao et al, 2013;Monteiro et al, 2015;Li et al, 2017;Jiang et al, 2018;Abbey et al, 2019;Calvo-Garrido et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…A number of bio-fungicides have recently been developed, but their efficacy against Botrytis blossom blight in lowbush blueberry production systems have not been evaluated. While adequate blight control has not been obtained when the products are used alone, preliminary studies have demonstrated that adequate disease suppression can be achieved when biofungicides are combined with the conventional fungicide by way of rotation (Percival et al, 2016, Abbey et al, 2020 and/or when conventional fungicides are used during peak disease pressures. In view of the continuous search for more economically and environmentally friendly alternative for conventional fungicides, it is important to evaluate new products that have been shown to suppress Botrytis cinerea in other crops.…”

Section: Introductionmentioning

confidence: 99%

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Potential use of biofungicides and conventional fungicide for the management of Botrytis blossom blight in lowbush blueberries

Abbey

Percival

Jaakola

et al. 2021

Canadian Journal of Plant Pathology

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Botrytis blight is an economically important disease of lowbush blueberry that causes significant yield loss annually. In this study, four biofungicides, Diplomat 5SC  (polyoxin D), Timorex Gold  (tea tree oil), Fracture  (BLAD) and Serenade MAX  (Bacillus subtilis) were evaluated for their disease suppression potential against B. cinerea individually and in rotation with Switch ® under field conditions. Three applications of each biofungicide were made for the stand alone treatment at 7-10 days' interval with Switch ® replacing the 2 nd application in the combined treatment. Resultsindicated that the products have potential for use as a biofungicide in lowbush blueberries. All the stand alone and rotational applications brought about significant reduction in disease development, especially in 2019. The application of Diplomat 5SC  and Fracture ® -Switch ® rotation decreased disease development by over 63% in 2018. In 2019, all stand alone treatments reduced disease development by more than 42% whereas their rotation with switch reduced disease by over 69% at Earltown and at least 30% at Farmington. Stand alone Diplomat 5SC ® and Timorex Gold ® along with Fracture ® , Timorex Gold ® and Serenade MAX  rotation resulted in over 20% more berries.This study suggests that the biofungicides and their integration with chemical fungicides have the potential as an alternative management strategy against Botrytis blossom blight to reduce the use of conventional fungicides and produce fruit with no detectable fungicide residues.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential use of biofungicides and conventional fungicide for the management of Botrytis blossom blight in lowbush blueberries

Abbey

Percival

Jaakola

et al. 2021

Canadian Journal of Plant Pathology

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Biocontrol Activity ofBacillusspp. andPseudomonasspp. AgainstBotrytis cinereaand Other Cannabis Fungal Pathogens

et al. 2022

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Gray mold caused by Botrytis cinerea is one of the most widespread and damaging diseases in cannabis crops worldwide. With challenging restrictions on pesticide use and few effective control measures, biocontrol agents are needed to manage this disease. The aim of this study was to identify and characterize bacterial biocontrol agents with wide-spectrum activity against B. cinerea and other major cannabis fungal pathogens. Twelve Bacillus and Pseudomonas strains were first screened with in vitro confrontational assays against ten culturable cannabis pathogens, namely B. cinerea, Sclerotinia sclerotiorum, Fusarium culmorum, F. sporotrichoides, F. oxysporum, Nigrospora sphaerica, N. oryzae, Alternaria alternata, Phoma sp. and Cercospora sp. Six strains displaying the highest inhibitory activity, namely B. velezensis LBUM279, FZB42, LBUM1082, B. subtilis LBUM979, P. synxantha LBUM223, and P. protegens Pf-5, were further assessed in planta where all, except LBUM223, significantly controlled gray mold development on cannabis leaves. Notably, LBUM279 and FZB42 reduced disease severity by at least half compared to water-treated plants and prevented lesion development and/or sporulation up to 9 days following pathogen inoculation. Genomes of LBUM279, LBUM1082 and LBUM979 were sequenced de novo and taxonomic affiliations were determined to ensure non-relatedness with pathogenic strains. Moreover, the genomes were exempt of detrimental genes encoding major toxins and virulence factors that could otherwise pose a biosafety risk when used on crops. Eighteen gene clusters of potential biocontrol interest were also identified. To our knowledge, this is the first reported attempt to control cannabis fungal diseases in planta by direct antagonism with beneficial bacteria.

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Cannabis Seedlings Inherit Seed-Borne Bioactive and Anti-Fungal Endophytic Bacilli

Dumigan

Deyholos

2022

Plants

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Throughout the hundreds of millions of years of co-evolution, plants and microorganisms have established intricate symbiotic and pathogenic relationships. Microbial communities associated with plants are in constant flux and can ultimately determine whether a plant will successfully reproduce or be destroyed by their environment. Inheritance of beneficial microorganisms is an adaptation plants can use to protect germinating seeds against biotic and abiotic stresses as seedlings develop. The interest in Cannabis as a modern crop requires research into effective biocontrol of common fungal pathogens, an area that has seen little research. This study examines the seed-borne endophytes present across 15 accessions of Cannabis grown to seed across Western Canada. Both hemp and marijuana seedlings inherited a closely related group of bioactive endophytic Bacilli. All Cannabis accessions possessed seed-inherited Paenibacillus mobilis with the capacity to solubilize mineral phosphate. Additionally, seeds were found to carry genera of fungal isolates known to be Cannabis pathogens and post-harvest molds: Alternaria, Penicillium, Cladosporium, Chaetomium, Aspergillus, Rhizopus, and Fusarium. Thirteen seed-borne endophytes showed antibiotic activity against Alternaria, Aspergillus, Penicillium, and Fusarium. This study suggests both fungal pathogens and bacterial endophytes that antagonize them are vectored across generations in Cannabis as they compete over this shared niche.

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Use of biofungicides to address conventional Botrytis blight control challenges in wild blueberry production

Cited by 4 publications

References 26 publications

Potential use of biofungicides and conventional fungicide for the management of Botrytis blossom blight in lowbush blueberries

Potential use of biofungicides and conventional fungicide for the management of Botrytis blossom blight in lowbush blueberries

Biocontrol Activity ofBacillusspp. andPseudomonasspp. AgainstBotrytis cinereaand Other Cannabis Fungal Pathogens

Cannabis Seedlings Inherit Seed-Borne Bioactive and Anti-Fungal Endophytic Bacilli

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