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Validation of reference genes. To confirm the reliability of selected reference genes, the relative expression profiles of PR3 gene was determined and normalized with the two most stable and two least stable genes. The relative expression levels were calculated by 2 −△△Ct method 49. For each qPCR experiment, three technical replicates were performed for each biological replicate. A one-way analysis of variance (ANOVA) was performed using the PROC GLIMMIX procedures of SAS (version 9.3, SAS institute, Inc., Cary, NC) for each time-point. Fisher's LSD was used for multiple means comparison at the level of α = 0.05.

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