Biofungicides as alternative to synthetic fungicide control of grey mould (<i>Botrytis cinerea</i>) – prospects and challenges

Abbey, Joel; Percival, David; Abbey, Lord; Asiedu, Samuel K.; Prithiviraj, Balakrishnan; Schilder, A. M. C.

doi:10.1080/09583157.2018.1548574

Cited by 192 publications

(138 citation statements)

References 109 publications

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“…Those epidemiological relationships were incorporated in a mechanistic model for B. cinerea-grapevine developed by González-Domínguez et al [47], but the latter model did not include a BCA component. The use of BCAs for BBR control has been extensively studied [61][62][63][64][65][66], with emphasis on biocontrol mechanisms and field efficacy; less research has been conducted to understand how environmental conditions affect BCA fitness and efficacy [11]. In the current study, the model parametrization for BCAs used different parameter values represented by nine BCA strains, which differed in their growth and survival in response to temperature and moisture conditions.…”

Section: Discussionmentioning

confidence: 99%

A Generic Model Accounting for the Interactions among Pathogens, Host Plants, Biocontrol Agents, and the Environment, with Parametrization for Botrytis cinerea on Grapevines

et al. 2020

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Although the use of biocontrol agents (BCAs) to manage plant pathogens has emerged as a sustainable means for disease control, global reliance on their use remains relatively insignificant and the factors influencing their efficacy remain unclear. In this work, we further developed an existing generic model for biocontrol of foliar diseases, and we parametrized the model for the Botrytis cinerea–grapevine pathosystem. The model was operated under three climate types to study the combined effects on BCA efficacy of four factors: (i) BCA mechanism of action, (ii) timing of BCA application with respect to timing of pathogen infection (preventative vs. curative), (iii) temperature and moisture requirements for BCA growth, and (iv) BCA survival capability. All four factors affected biocontrol efficacy, but factors iii and iv accounted for > 90% of the variation in model simulations. In other words, the most important factors affecting BCA efficacy were those related to environmental conditions. These findings indicate that the environmental responses of BCAs should be considered during their selection, BCA survival capability should be considered during both selection and formulation, and weather conditions and forecasts should be considered at the time of BCA application in the field.

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Section: Discussionmentioning

confidence: 99%

A Generic Model Accounting for the Interactions among Pathogens, Host Plants, Biocontrol Agents, and the Environment, with Parametrization for Botrytis cinerea on Grapevines

et al. 2020

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“…F. graminearum causes Fusarium head blight in different cereal crops (37). B. cinerea has a very wide variety of hosts, however, its main hosts are wine grapes and other fruits where it is causing the grey mold disease (38,39 The qualitative assessment of antifungal potential from 22 tested isolates was classified into inhibition, minor inhibition and no inhibition by comparing mutant strains to their respectively wild types and wild types with each other ( Fig. 2A, Fig.…”

Section: Introductionmentioning

confidence: 99%

“…F. oxysporum, F. graminearum and B. cinerea were revived on Potato Dextrose Agar (PDA, BD, USA, potato infusion 4 g l −1 , glucose 20 g l −1 , agar 15 g l −1 ) supplemented with 0.5 g l−1 CuSO4 and 0.5 g l−1 ZnSO4 to harvest spores. B. subtilis mutant strain construction.Strains 23,38,39,64, 72, 73, 75 and 77 were isolated specifically by labelling with constitutively expressed gfp from Phyperspank using phyGFP plasmid that integrates into the amyE locus(54). Mutant strains were obtained using natural competence (55) by transforming genomic DNA and selecting for antibiotic resistance, followed by verifying the mutation by PCR.…”

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confidence: 99%

Genomic and chemical diversity ofBacillus subtilissecondary metabolites against plant pathogenic fungi

Kiesewalter

Lozano-Andrade

Wibowo

et al. 2020

Preprint

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Bacillus subtilis produces a wide range of secondary metabolites providing diverse plant-growth-promoting and biocontrol abilities. These secondary metabolites include non-ribosomal peptides (NRPs) with strong antimicrobial properties, causing either cell lysis, pore formation in fungal membranes, inhibition of certain enzymes, or bacterial protein synthesis. However, the natural products of B. subtilis are mostly studied either in laboratory strains or in individual isolates and therefore, a comparative overview of B. subtilis secondary metabolites is missing.In this study, we have isolated 23 B. subtilis strains from eleven sampling sites, compared the fungal inhibition profiles of wild types and their NRPs mutants, followed the production of targeted lipopeptides, and determined the complete genomes of 13 soil isolates. We discovered that non-ribosomal peptide production varied among B. subtilis strains co-isolated from the same soil samples. In vitro antagonism assays revealed that biocontrol properties depend on the targeted plant pathogenic fungus and the tested B. subtilis isolate. While plipastatin alone is sufficient to inhibit Fusarium sp., a combination of plipastatin and surfactin is required to hinder the growth of Botrytis cinerea. Detailed genomic analysis revealed that altered NRP production profiles in certain isolates is due to missing core genes, nonsense mutation, or potentially altered gene regulation.Our study combines microbiological antagonism assays with chemical NRPs detection and biosynthetic gene cluster predictions in diverse B. subtilis soil isolates to provide a broader overview of the secondary metabolite chemodiversity of B. subtilis.IMPORTANCESecondary or specialized metabolites with antimicrobial activities define the biocontrol properties of microorganisms. Members of the Bacillus genus produce a plethora of secondary metabolites, of which non-ribosomally produced lipopeptides in particular display strong antifungal activity. To facilitate prediction of the biocontrol potential of new Bacillus subtilis isolates, we have explored the in vitro antifungal inhibitory profiles of recent B. subtilis isolates, combined with analytical natural product chemistry, mutational analysis, and detailed genome analysis of biosynthetic gene clusters. Such a comparative analysis helped to explain why selected B. subtilis isolates lack production of certain secondary metabolites.

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“…Biofungicida é o nome dado aos microorganismos e extratos/óleos de plantas naturais que tem a capacidade de controlar doenças de plantas (Abbey et al, 2018). Os microorganismos mais utilizados no controle de doenças pós-colheita em maçã são:…”

Section: Colheita Em Maçãunclassified

Podridões pós-colheita em maçã: perdas econômicas e alternativas de manejo

Ogoshi

Argenta

Monteiro

et al. 2019

BJD

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Biofungicides as alternative to synthetic fungicide control of grey mould (Botrytis cinerea) – prospects and challenges

Cited by 192 publications

References 109 publications

A Generic Model Accounting for the Interactions among Pathogens, Host Plants, Biocontrol Agents, and the Environment, with Parametrization for Botrytis cinerea on Grapevines

A Generic Model Accounting for the Interactions among Pathogens, Host Plants, Biocontrol Agents, and the Environment, with Parametrization for Botrytis cinerea on Grapevines

Genomic and chemical diversity ofBacillus subtilissecondary metabolites against plant pathogenic fungi

Podridões pós-colheita em maçã: perdas econômicas e alternativas de manejo

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