Use of bioluminescence mutant screening for identification of Edwardsiella ictaluri genes involved in channel catfish (Ictalurus punctatus) skin colonization

Menanteau–Ledouble, Simon; Lawrence, Mark L.

doi:10.1016/j.vetmic.2012.09.024

Cited by 10 publications

(13 citation statements)

References 43 publications

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“…Studies of the role of RstAB system in fish pathogens are very scarce. Notably, mutation of rstB in Edwardsiella ictaluri caused impaired colonization and virulence in a channel catfish model (Menanteau-Ledouble and Lawrence, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…rstAB genes are considered to be part of the PhoP/PhoQ regulon (a Mg 2+ -sensing two-component system) (Oshima et al, 2002; Ogasawara et al, 2007). While some evidence suggests that the RstAB system might respond to acidic conditions (Ogasawara et al, 2007; Huang et al, 2018), the specific stimuli that trigger the activation of the sensor histidine kinase RstB remain unknown for all the RstAB-like systems studied to date (Ogasawara et al, 2007; Campbell et al, 2007; Flamez et al, 2008; Bilecen and Yildiz, 2009; Menanteau-Ledouble and Lawrence, 2013; Tran et al, 2016; Huang et al, 2018). In contrast, a number of genes and biological functions under control of the RstAB system have been identified, demonstrating that the RstAB regulon is not predictive since it shows a high diversity among species.…”

Section: Discussionmentioning

confidence: 99%

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The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae

et al. 2019

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The RstB histidine kinase of the two component system RstAB positively regulates the expression of damselysin (Dly), phobalysin P (PhlyP) and phobalysin C (PhlyC) cytotoxins in the fish and human pathogen Photobacterium damselae subsp. damselae , a marine bacterium of the family Vibrionaceae . However, the function of the predicted cognate response regulator RstA has not been studied so far, and the role of the RstAB system in other cell functions and phenotypes remain uninvestigated. Here, we analyzed the effect of rstA and rstB mutations in cell fitness and in diverse virulence-related features. Both rstA and rstB mutants were severely impaired in virulence for sea bream and sea bass fish. Mutants in rstA and rstB genes were impaired in hemolysis and in Dly-dependent phospholipase activity but had intact PlpV-dependent phospholipase and ColP-dependent gelatinase activities. rstA and rstB mutants grown at 0.5% NaCl exhibited impaired swimming motility, enlarged cell size and impaired ability to separate after cell division, whereas at 1% NaCl the mutants exhibited normal phenotypes. Mutation of any of the two genes also impacted tolerance to benzylpenicillin. Notably, rstA and rstB mutants showed impaired secretion of a number of type II secretion system (T2SS)-dependent proteins, which included the three major cytotoxins Dly, PhlyP and PhlyC, as well as a putative delta-endotoxin and three additional uncharacterized proteins which might constitute novel virulence factors of this pathogenic bacterium. The analysis of the T2SS-dependent secretome of P. damselae subsp. damselae also led to the identification of RstAB-independent potential virulence factors as lipoproteins, sialidases and proteases. The RstAB regulon included plasmid, chromosome I and chromosome II-encoded genes that showed a differential distribution among isolates of this subspecies. This study establishes RstAB as a major regulator of virulence and diverse cellular functions in P. damselae subsp. damselae .

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae

et al. 2019

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“…Several studies demonstrated that rstA and rstB are closely associated with bacterial virulence (Menanteau‐Ledouble & Lawrence, ). For example, rstA and rstB are involved in the regulation of invasion genes in Salmonella enterica Typhimurium (Menanteau‐Ledouble & Lawrence, ). The critical role of rstB in P. damselae subsp.…”

Section: Discussionmentioning

confidence: 99%

“…Tran, Han, Shi, and Guo () found that invasion, motility, pyrimidine metabolism, and iron acquisition in Salmonella typhimurium are controlled by the RstB‐RstA system. Menanteau‐Ledouble and Lawrence () demonstrated that mutation of the RstB‐RstA system results in decreased adhesion and virulence in Edwardsiella ictaluri . Terceti et al.…”

Section: Introductionmentioning

confidence: 99%

Regulatory role of the RstB‐RstA system in adhesion, biofilm production, motility, and hemolysis

Huang

Xü

et al. 2018

MicrobiologyOpen

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For infection, initial invasion of the host is of great importance, with adhesion playing a critical role. We previously demonstrated rstA and rstB are remarkably downregulated in Vibrio alginolyticus cultured under heavy metal and acidic stresses, with impaired adhesion, suggesting that rstA and rstB might be involved in adhesion regulation. The present study showed that rstA and rstB silencing resulted in impaired adhesion, biofilm production, motility, hemolysis, and virulence. Meanwhile, changes of temperature, starvation, and pH remarkably affected rstA and rstB expression. These findings indicated that (1) rstA and rstB are critical regulators of adhesion in V. alginolyticus; (2) rstA and rstB have remarkable effects on biofilm production, motility, hemolysis, and virulence in V. alginolyticus; (3) rstA and rstB modulate adhesion in response to environmental changes of temperature, pH, and starvation.

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“…Developing a fully tunable dynamic expression platform will require a more complete understanding of the underlying processes. Plasmid-loss dynamics have been well described in a variety of in vitro and in vivo contexts; 20 , 12 , 15 however, modeling of population or gene-expression dynamics has not yet been studied for in vivo tumor environments. Specifically, we hope to learn how expression dynamics are dictated by the rates of growth and plasmid-loss for a given strain.…”

mentioning

confidence: 99%

In Vivo Gene Expression Dynamics of Tumor-Targeted Bacteria

Danino

Prindle³

et al. 2012

ACS Synth. Biol.

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The engineering of bacteria to controllably deliver therapeutics is an attractive application for synthetic biology. While most synthetic gene networks have been explored within microbes, there is a need for further characterization of in vivo circuit behavior in the context of applications where the host microbes are actively being investigated for efficacy and safety, such as tumor drug delivery. One major hurdle is that culture-based selective pressures are absent in vivo, leading to strain-dependent instability of plasmid-based networks over time. Here, we experimentally characterize the dynamics of in vivo plasmid instability using attenuated strains of S. typhimurium and real-time monitoring of luminescent reporters. Computational modeling described the effects of growth rate and dosage on live-imaging signals generated by internal bacterial populations. This understanding will allow us to harness the transient nature of plasmid-based networks to create tunable temporal release profiles that reduce dosage requirements and increase the safety of bacterial therapies.

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Use of bioluminescence mutant screening for identification of Edwardsiella ictaluri genes involved in channel catfish (Ictalurus punctatus) skin colonization

Cited by 10 publications

References 43 publications

The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae

The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae

Regulatory role of the RstB‐RstA system in adhesion, biofilm production, motility, and hemolysis

In Vivo Gene Expression Dynamics of Tumor-Targeted Bacteria

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