Use of California mastitis test, somatic cells count and bacteriological findings in diagnostics of subclinical mastitis

Varatanović, Nazif; Podžo, Mustafa; Mutevelić, Tarik; Podžo, Kenan; Čengić, Benjamin; Hodžić, Aida; Hodzic, E.

doi:10.2298/bah1002065v

Cited by 6 publications

(4 citation statements)

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“…38.4%, and for 2017. 47.7%) coincides with results of similar earlier studies conducted in BiH (Varatanović, 2010). Comparative studies from other countries report range of established prevalence; from 21.5% in Ethiopia (Workineh, 2002), 26.4% in Germany (Terhagen, 2006), 31% in Finland (Pitkälä, 2004), up to 52.4% in Uruguay (Gianneechini, 2002).…”

Section: Resultssupporting

confidence: 87%

“…In addition to burden of infectious mastitis which causative agents have public health importance, mastitis related production losses are one of the most important limiting factors of dairy production in Bosnia and Herzegovina (BiH) (Alagić, 2006). Prevalence of either clinical or subclinical mastitis on farms in BiH can reach more than 50% (Varatanović, 2010). Most commonly about half of these cases are clinical mastitis, where most commonly isolated causative agents are Staphylococcus aureus, Streptococcus agalactiae, coagulase positive staphylococci, Trueperellapyogenesand coliforms (Matarugić, 2009;Varatanović, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Farm records in investigation of epidemiology, symptomatology and causes of clinical mastitis in a dairy farm

et al. 2019

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Mastitis is one of the most important diseases in dairy cow farms and one of the most common cause for antibiotic treatment. Aims of this study were: to investigate frequency and trends of clinical mastitis in cows on a large dairy farm, describe clinical characteristics of mastitis and investigate causative infectious agents in selected cases alongside antimicrobial resistance. In our study we used farm records for clinical mastitis recorded for period 2016 and 2017. We also used results of the regular on farm testing of the somatic cell count for 2017. Samples of milk from all clinical mastitis cases were taken during November and December 2017 in order to investigate causative agents and their antimicrobial resistance. Occurrence of clinical mastitis was 205 cases (47.7%) in 2017 compared to 93 cases (29.7%) recorded in 2016. In 2017 reoccurrence of clinical mastitis in same animal was recorded for 93 cows (45.4%). In 2016 reoccurrence of clinical mastitis in same animal was recoded for 49 cows (29.7%). Average course of clinical mastitis in 2016 was 3 days, while in 2017 4.5 days (continuous days of recording a case in farm records). Somatic cell count in more than half of tested animals was higher than 200.000 SC/ml according to the measurements from February and July 2017 (number of cows tested 236 and 169, respectively). Out of 23 milk samples, 20 had bacteriological growth. In 9 samples we identified S.aureus, in 6 streptococcus spp., in 4 coagulase negative staphylococci (CNS) and one sample contained E.coli. Most common resistance was found for lincomycinspectinomycin (100%) gentamicin (92%), followed by cefquinome (65%), linkomycin (53%) and erythromycin (47%). Isolates of S.aureus were resistant on the largest number of investigated antibiotics.

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Farm records in investigation of epidemiology, symptomatology and causes of clinical mastitis in a dairy farm

et al. 2019

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“…In dairy industry, intramammary infections are among the most important diseases of cows that cause great economic losses [ 30 ]. Mastitis is a response of udder to different internal and external factors [ 31 ], and substantially, affects the milk quality and production of dairy cows. Bacterial pathogens are major threat to mammary gland, which causes irritation and pathological changes in mammary tissue.…”

Section: Immuno-pathogenesismentioning

confidence: 99%

Molecular markers for resistance against infectious diseases of economic importance

et al. 2017

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Huge livestock population of India is under threat by a large number of endemic infectious (bacterial, viral, and parasitic) diseases. These diseases are associated with high rates of morbidity and mortality, particularly in exotic and crossbred cattle. Beside morbidity and mortality, economic losses by these diseases occur through reduced fertility, production losses, etc. Some of the major infectious diseases which have great economic impact on Indian dairy industries are tuberculosis (TB), Johne’s disease (JD), mastitis, tick and tick-borne diseases (TTBDs), foot and mouth disease, etc. The development of effective strategies for the assessment and control of infectious diseases requires a better understanding of pathogen biology, host immune response, and diseases pathogenesis as well as the identification of the associated biomarkers. Indigenous cattle (Bos indicus) are reported to be comparatively less affected than exotic and crossbred cattle. However, genetic basis of resistance in indigenous cattle is not well documented. The association studies of few of the genes associated with various diseases, namely, solute carrier family 11 member 1, Toll-like receptors 1, with TB; Caspase associated recruitment domain 15, SP110 with JD; CACNA2D1, CD14 with mastitis and interferon gamma, BoLA-DRB3.2 alleles with TTBDs, etc., are presented. Breeding for genetic resistance is one of the promising ways to control the infectious diseases. High host resistance is the most important method for controlling such diseases, but till today no breed is total immune. Therefore, work may be undertaken under the hypothesis that the different susceptibility to these diseases are exhibited by indigenous and crossbred cattle is due to breed-specific differences in the dealing of infected cells with other immune cells, which ultimately influence the immune response responded against infections. Achieving maximum resistance to these diseases is the ultimate goal, is technically possible to achieve, and is permanent. Progress could be enhanced through introgression of resistance genes to breeds with low resistance. The quest for knowledge of the genetic basis for infectious diseases in indigenous livestock is strongly warranted.

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“…(5) ve Varatanovic ve ark. (17), bildirdiği oranlar ile benzerdir fakat + ve ++ reaksiyon veren meme loblarından elde edilen oranlar arasında farklılık mevcuttur. Bu farklılığın nedeni diğer çalışmalarda (11,14,15) da açıklandığı gibi CMT'nin farklı bakterilere bağlı mastitislerin saptanmasındaki spesifite ve sensivitesine bağlı olabilir.…”

Section: Tartışma Ve Sonuçunclassified

Kuru döneme çıkarken enfekte ve sağlıklı meme loblarında California Mastitis Test ve somatik hücre sayısı ile bakteriyolojik muayene sonuçlarının karşılaştırılması

Alkan¹,

Baştan²,

Salar³

et al. 2014

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışmada kuruya çıkarma sırasında California Mastitis Test (CMT) sonuçlarının somatik hücre sayımı (SHS) ve bakteriyolojik muayene sonuçları ile karşılaştırılması ve CMT'nin kuru dönem başlangıcında enfekte meme loblarının belirlenmesinde etkinliğinin ortaya konması amaçlandı. Bu amaçla, 28 adet Holştayn ırkı ineğe ait 112 meme lobundan alınan 109 adet süt örneği değerlendirildi, 3 meme kör olduğu için süt örneği alınamadı. Kuruya çıkarmadan önceki son sağımda klinik muayenede normal olan meme loblarından alınan süt örneklerine CMT yapıldı ve sonuçlar aynı kişi tarafından yorumlandı. Bu uygulamadan sonra her meme lobundan SHS ve bakteriyolojik muayene için süt örnekleri alındı. CMT sonucuna göre 83 meme lobu negatif (N), 8 meme lobu şüpheli (T), 15 meme lobu + ve 3 meme lobu ise ++ reaksiyon verdi. CMT ile SHS sonuçları karşılaştırıldığında, CMT negatif meme loblarında ortalama SHS 155.178 hücre/ml, şüphelilerde 221.750 hücre/ml, + ve ++ reaksiyon verenlerde sırasıyla 635.400 ve 1.782.000 hücre/ml idi. Herhangi bir bakteri üreyen ve üremeyen meme loblarında ortalama somatik hücre sayıları ise sırasıyla 195.350 hücre/ml ve 304.400 hücre/ml idi. Bakteri üremesi olan ve olmayan gruplara ait ortalama somatik hücre sayıları arasındaki fark istatistiksel açıdan anlamlı değildi (p>0.05). Sonuç olarak kuruya çıkarılan ineklerde subklinik meme içi enfeksiyonların belirlenmesinde CMT'nin tek başına yeterli olmadığı, CMT sonuçlarının bakteriyolojik muayene sonuçları ile birlikte değerlendirilmesi gerektiği kanısına varıldı.Anahtar sözcükler: Bakteriyolojik muayene, CMT, kuru dönem, somatik hücre sayısı, sütçü inek. Comparision of results of California Mastitis Test, somatic cell counts and microbiological examination in healthy and infected quarter in cows at dry-off. Summary: The aim of this study was to compare California Mastitis Test (CMT) findings with Somatic Cell Count (SCC)and bacteriological examination at the dry-off, and to investigate the effectiveness of the CMT to detect the infected quarters at the beginning of the dry period. For this purpose, 109 milk samples collected from 112 udder quarters of 28 Holstein cows were evaluated, 3 milk samples could not take from these quarters owing to blindness. CMT was made on milk samples taking from clinically normal quarters before the last milking and the results was interpreted by the same person. After this examination milk samples were collected from each quarter for SCC and bacteriological isolation. Of the samples, 83 were CMT (-), 8 were trace (T), 15 were CMT (+), 3 were CMT (++). According to comparision of CMT and SCC results, the mean SCC was 155.178 cell/ml, 221.750 cell/ml, 635.400 cell/ml, 1.782.000 cell/ml in CMT (-), trace (T), CMT (+), CMT (++) quarters, respectively. The mean SCC of any bacteria isolated and non-isolated udder quarters were 195.350 cell/ml and 304.400 cell/ml, respectively. Difference in SCC levels between infected and non-infected groups was not significant statistically (p>0.05). In conclusion, only CMT was not adequate t...

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Use of California mastitis test, somatic cells count and bacteriological findings in diagnostics of subclinical mastitis

Cited by 6 publications

References 3 publications

Farm records in investigation of epidemiology, symptomatology and causes of clinical mastitis in a dairy farm

Farm records in investigation of epidemiology, symptomatology and causes of clinical mastitis in a dairy farm

Molecular markers for resistance against infectious diseases of economic importance

Kuru döneme çıkarken enfekte ve sağlıklı meme loblarında California Mastitis Test ve somatik hücre sayısı ile bakteriyolojik muayene sonuçlarının karşılaştırılması

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