Use of Carboxylated Microspheres to Assess Transport Potential of<i>Cryptosporidium parvum</i>Oocysts at the Russian River Water Supply Facility, Sonoma County, California

Metge, David W.; Harvey, Ronald W.; Anders, Robert A.; Rosenberry, Donald O.; Seymour, D.; Jasperse, J.

doi:10.1080/01490450701456867

Cited by 16 publications

(19 citation statements)

References 39 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oocysts were stained by contacting them with DNA-specific fluorescent dye 4,6-diamidino 2-phynulindole (DAPI, 0.1 mg L −1 solution) for 30 min prior to counting them manually with epifluorescence microscopy. The excitation and emission wavelengths used for DAPI-stained oocysts were 350 and 470 nm, respectively [9,33]. Average oocyst size was determined by immunofluorescent flow cytometry analysis of DAPI-stained samples (Biorad, HS Bryte) [33].…”

Section: Cryptosporidium Oocysts Characterizationmentioning

confidence: 99%

See 1 more Smart Citation

In-situ monitoring of Cryptosporidium parvum oocyst surface adhesion using ATR-FTIR spectroscopy

Gao

Chorover

2009

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Section: Cryptosporidium Oocysts Characterizationmentioning

confidence: 99%

“…The excitation and emission wavelengths used for DAPI-stained oocysts were 350 and 470 nm, respectively [9,33]. Average oocyst size was determined by immunofluorescent flow cytometry analysis of DAPI-stained samples (Biorad, HS Bryte) [33]. The average spherical diameter of oocysts was calculated to be 3.88 m.…”

Section: Cryptosporidium Oocysts Characterizationmentioning

confidence: 99%

In-situ monitoring of Cryptosporidium parvum oocyst surface adhesion using ATR-FTIR spectroscopy

Gao

Chorover

2009

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

“…The study was conducted in a section of the pond where pond water continuously recharges a sandy, sole‐source, drinking‐water aquifer. For this natural‐gradient transport experiment, we used a Lee‐type (bag‐and‐barrel) flow meter (Lee, 1977) modified from that described in an earlier colloidal transport study at the Russian River, California RBF site (Metge et al, 2007). Push‐point samplers were driven into the sediments to depths corresponding to specific locations along the first meter of a flow path determined a priori using a conservative (bromide) tracer.…”

mentioning

confidence: 99%

Importance of the Colmation Layer in the Transport and Removal of Cyanobacteria, Viruses, and Dissolved Organic Carbon during Natural Lake-Bank Filtration

Harvey¹,

Metge²,

LeBlanc³

et al. 2015

J. Environ. Qual.

Self Cite

View full text Add to dashboard Cite

This study focused on the importance of the colmation layer in the removal of cyanobacteria, viruses, and dissolved organic carbon (DOC) during natural bank filtration. Injection-and-recovery studies were performed at two shallow (0.5 m deep), sandy, near-shore sites at the southern end of Ashumet Pond, a waste-impacted, kettle pond on Cape Cod, MA, that is subject to periodic blooms of cyanobacteria and continuously recharges a sole-source drinking-water aquifer. The experiment involved assessing the transport behaviors of bromide (conservative tracer), sp. IU625 (cyanobacterium, 2.6 ± 0.2 µm), AS-1 (tailed cyanophage, 110 nm long), MS2 (coliphage, 26 nm diameter), and carboxylate-modified microspheres (1.7 µm diameter) introduced to the colmation layer using a bag-and-barrel (Lee-type) seepage meter. The injectate constituents were tracked as they were advected across the pond water-groundwater interface and through the underlying aquifer sediments under natural-gradient conditions past push-point samplers placed at ∼30-cm intervals along a 1.2-m-long, diagonally downward flow path. More than 99% of the microspheres, IU625, MS2, AS-1, and ∼44% of the pond DOC were removed in the colmation layer (upper 25 cm of poorly sorted bottom sediments) at two test locations characterized by dissimilar seepage rates (1.7 vs. 0.26 m d). Retention profiles in recovered core material indicated that >82% of the attached IU625 were in the top 3 cm of bottom sediments. The colmation layer was also responsible for rapid changes in the character of the DOC and was more effective (by three orders of magnitude) at removing microspheres than was the underlying 20-cm-thick segment of sediment.

show abstract

“…Successful surrogates should be safe to release and easy to detect. Fluorescent polystyrene microspheres are nontoxic, inert particles that have been utilized as surrogates for other pathogens, including the protozoan parasite Cryptosporidium, viruses, and bacteria (18,22,26,38). Using surrogates in the laboratory and field requires that particles must accurately predict the behavior of T. gondii by mimicking important physical and chemical properties.…”

mentioning

confidence: 99%

Surface Properties of Toxoplasma gondii Oocysts and Surrogate Microspheres

Shapiro

Largier

Mazet

et al. 2009

Appl Environ Microbiol

View full text Add to dashboard Cite

The physical properties that govern the waterborne transmission of Toxoplasma gondii oocysts from land to sea were evaluated and compared to the properties of carboxylated microspheres, which could serve as surrogates for T. gondii oocysts in transport and water treatment studies. The electrophoretic mobilities of T. gondii oocysts, lightly carboxylated Dragon Green microspheres, and heavily carboxylated Glacial Blue microspheres were determined in ultrapure water, artificial freshwater with and without dissolved organic carbon, artificial estuarine water, and artificial seawater. The surface wettabilities of oocysts and microspheres were determined using a water contact angle approach. Toxoplasma gondii oocysts and microspheres were negatively charged in freshwater solutions, but their charges were neutralized in estuarine water and seawater. Oocysts, Glacial Blue microspheres, and unwashed Dragon Green microspheres had low contact angles, indicating that they were hydrophilic; however, once washed, Dragon Green microspheres became markedly hydrophobic. The hydrophilic nature and negative charge of T. gondii oocysts in freshwater could facilitate widespread contamination of waterways. The loss of charge observed in saline waters may lead to flocculation and subsequent accumulation of T. gondii oocysts in locations where freshwater and marine water mix, indicating a high risk of exposure for humans and wildlife in estuarine habitats with this zoonotic pathogen. While microspheres did not have surface properties identical to those of T. gondii, similar properties shared between each microsphere type and oocysts suggest that their joint application in transport and fate studies could provide a range of transport potentials in which oocysts are likely to behave.

show abstract

Use of Carboxylated Microspheres to Assess Transport Potential ofCryptosporidium parvumOocysts at the Russian River Water Supply Facility, Sonoma County, California

Cited by 16 publications

References 39 publications

In-situ monitoring of Cryptosporidium parvum oocyst surface adhesion using ATR-FTIR spectroscopy

In-situ monitoring of Cryptosporidium parvum oocyst surface adhesion using ATR-FTIR spectroscopy

Importance of the Colmation Layer in the Transport and Removal of Cyanobacteria, Viruses, and Dissolved Organic Carbon during Natural Lake-Bank Filtration

Surface Properties of Toxoplasma gondii Oocysts and Surrogate Microspheres

Contact Info

Product

Resources

About