Use of cell morphology as an early bio-sensor for viral infection

Abstract

This paper reports a correlation between cellular morphology and the ability of adapting Vesicular stomatitis virus (VSV) infection. A time-lapse approach was employed to track the individual difference between homologous cells in adopting viral infection. Our single-cell analysis indicates that upon viral infection, mature cells that are in spindle shape are less likely to be infected after 24 hour infection. On the other hand, cells undergoing proliferation, which are in rounder shape, tend to adopt much higher viral infection within the same amount of time. This fact suggests cellular morphology may to be an early bio-sensor for viral infection. The findings in this paper could potentially be applied to other viral infection models.

JALA Special Issue: New Developments in Biosensing Technologies

“…Biosensors have been widely applied in clinical food analysis, fermentation industrial control, environmental monitoring, defense, and other areas of safety testing. For instance, biosensors are vital elements for the amino acid industry [7][8][9] (synthesis of monosodium glutamate, aspartate, alanine, and lysine), antibiotics industry 10,11 (glucose online monitoring and control systems), alcohol industry, 12,13 enzymes industry [14][15][16] (glucoamylase rapid analysis), starch sugar industry 17 (glucose, starch, and glucoamylase analysis), biological cell cultures 18,19 (glucose, lactate, and glutamine analysis), microbial desulfurization cell culture monitoring in the petrochemical industry, 20,21 vitamin production, 22 fermentation of glycerol production, 23 and more. Biosensor detection technology is now an important tool for the enterprise transformation of bioprocessing companies.…”