Use of Cellulose Acetate Electrophoresis as an Alternative to Starch Gel Electrophoresis for Detecting Root-Knot Nematode Resistance in Tomato

Bolkan, Hassan

doi:10.1094/pd-71-1001

Cited by 6 publications

(4 citation statements)

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“…Protein separation in this medium can be performed usually within 15-40 min without cooling while bands resolution is only a little bit lower than that which is obtained using polyacrylamide gels (Richardson 1986). Such membranes are mainly used for routine procedures in clinical laboratories where speed of an analysis is of a considerable value but can also be success~hlly applied for plant material (Bolkan et al 1987).…”

Section: Introductionmentioning

confidence: 99%

Application of a rapid glucose-6-phosphate isomerase assay in white cabbage breeding

Barański

2000

Acta Physiol Plant

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In the studied conditions of cellulose acetate electrophoresis only glucose-6-phosphate isomerase (PGI, EC 5.3.1.9) appeared to be a polymorphic isozyme. The PGI analysis of 23 cabbage (Brassica oleracea var. capitata) inbred lines, carried out in two years, allowed detecting off-type individuals in four lines. Seed contamination caused by sib-pollination was detected in 10 out of 19 Ft hybrids. In five of them the contamination did not exceed 5 %, and in the remaining five, ranged from 27-52 %. Also homozygosity of 67 plants obtained through anther culture in vitro was confirmed. The results indicate that PGI electrophoresis in cellulose acetate matrix is very fast and can be useful in the assessment of genetic purity in cabbage breeding materials.

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Section: Introductionmentioning

confidence: 99%

Application of a rapid glucose-6-phosphate isomerase assay in white cabbage breeding

Barański

2000

Acta Physiol Plant

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“…Markers allow for the visualization and selection of rare recombination events that would be difficult to identify using trait data. MAS has been effectively used in tomato for decades as a technique to introduce novel resistance from a wild tomato relative to an adapted variety and to pyramid resistance genes (Bolkan et al 1987;Rick 1974). MAS was used to combine the resistance genes Rx-3 and Pto on chromosome 5, conferring resistance to Xanthomonas euvesicatoria race T1 and Pseudomonas syringae pv.…”

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confidence: 99%

Marker-assisted Selection to Combine Alleles for Four Disease Resistance Genes of Tomato Collocated on Chromosome 11

Orchard

Kressin²,

Chompookam³

et al. 2023

horts

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Bacterial, fungal, and viral diseases of tomato (Solanum lycopersicum) are responsible for widespread yield losses, especially in humid growing environments. Chromosome 11 of tomato contains genes that modulate resistance to several prominent tomato pathogens, including bacterial spot caused by Xanthomonas spp., gray leaf spot caused by Stemphylium spp., Fusarium wilt caused by race 2 of Fusarium oxysporum f. sp. lycopersici, and tomato yellow leaf curl virus (TYLCV) caused by begomoviruses. Major resistance loci are quantitative trait locus 11 (QTL-11) and Xv3/Rx4 for bacterial spot, Sm for gray leaf spot, I2 for Fusarium wilt, and Ty-2 for TYLCV. Marker-assisted selection was used to select for rare recombination events that combined these resistance loci into a linked cassette that can be inherited together in future crosses. A pedigree breeding strategy was used with marker-assisted selection and used to identify a novel coupling of Xv3/Rx4 and Ty-2. Recombination between the two genes was estimated as 0.056 cM, demonstrating that effective combinations of resistance can be established using publicly available germplasm. Progeny from the recombinant plants were screened using inoculated seedling trials to confirm resistance. The recombinants identified maintained resistance levels similar to the resistant controls. Trial results suggest that the trait markers on chromosome 11 are tightly linked to the respective resistance loci and are effective for selecting plants with resistance to the target diseases.

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“…Two alleles ofAps-l, designated Aps-I' and Aps-l', are found in domestic tomato. The encoded proteins, Apase-1+ and Apase-1' are separated by starch gel or cellulose acetate electrophoresis (6). Apase-1 is the electrophoretic form commonly found in the cultivated tomato, Lycopersicon esculentum.…”

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confidence: 99%

Acid Phosphatase-1 from Nematode Resistant Tomato

Williamson

Colwell²

1991

Plant Physiol.

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Use of Cellulose Acetate Electrophoresis as an Alternative to Starch Gel Electrophoresis for Detecting Root-Knot Nematode Resistance in Tomato

Cited by 6 publications

References 1 publication

Application of a rapid glucose-6-phosphate isomerase assay in white cabbage breeding

Application of a rapid glucose-6-phosphate isomerase assay in white cabbage breeding

Marker-assisted Selection to Combine Alleles for Four Disease Resistance Genes of Tomato Collocated on Chromosome 11

Acid Phosphatase-1 from Nematode Resistant Tomato

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