Use of Chemically Modified Silica with β-Diketoamine Groups for Separation of α-Lactoalbumin from Bovine Milk Whey by Affinity Chromatography

Gambero, Alessandra; Kubota, Lauro T.; Gushikem, Yoshitaka; Airoldi, Cláudio; Granjeiro, José Mauro; Taga, Eulázio Mikio; Alcântara, Edésio F.C.

doi:10.1006/jcis.1996.4566

Cited by 23 publications

(15 citation statements)

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“…For grafting aromatic groups onto the silica surface, the use of an intermediate reagent such as 3-chloropropyltrimethoxysilane or 3-aminopropyltrimethoxysilane, as bridging molecules was necessary 2,[5][6][7][8] . After the intermediate reactions the aromatic groups could be immobilized.…”

Section: Resultsmentioning

confidence: 99%

“…Stable bonded organic groups on a silica gel surface can be obtained using a grafting synthesis. The organosilane Y 3 Si-R-X is a very common agent used in the grafting process 2,[5][6][7][8] , Y is usually a halide or alkoxy group, R a linear chain and X an halide or amine group. Using a SN 2 reaction, the immobilization of several organic groups can be achieved.…”

Section: Introductionmentioning

confidence: 99%

“…The covalent bond between the aromatic groups and the silica surface was shown by the high thermal stability of these groups. 5,6 Silica gel (Merck) with 10 nm pore diameter, 0.02 -0.05 mm particle size and a surface area of 320 m 2 g -1 was activated at 150 o C under vacuum (10 -1 Pa) for 5 h. The 3-chloropropyltrimethoxysilane or 3-aminopropyltrimethoxysilane (Merck) (46.5 mmol) was dissolved in 300 ml of toluene (Merck). The activated silica (50 g) was then added.…”

Section: Introductionmentioning

confidence: 99%

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FTIR thermal analysis on organofunctionalized silica gel

Foschiera

Pizzolato

Benvenutti

2001

J. Braz. Chem. Soc.

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Sílica gel modificada com grupos orgânicos tem sido muito utilizada como fase estacionária para cromatografia líquida. Reações de enxerto podem ser usadas na obtenção de superfícies modificadas estáveis de sílica gel. Neste trabalho a sílica gel (10 nm de diâmetro de poro e 320 m 2 g -1 de área superficial) foi modificada quimicamente com 3-cloropropiltrimetoxisilano ou com 3-aminopropiltrimetoxisilano e posteriormente com anilina, p-anisidina, benzilamina e 3-fenilpropilcloreto a fim de se obter grupos aromáticos imobilizados na superfície de sílica gel. A espectroscopia no infravermelho foi utilizada para a caracterização dos grupos aromáticos enxertados, usando-se uma cela de quartzo. Os sólidos foram aquecidos a várias temperaturas em alto vácuo sendo que as áreas sob as bandas dos grupos orgânicos obtidas no infravermelho foram usadas para estimar a estabilidade térmica.Silica gel modified with organic groups is widely used as a stationary phase for liquid chromatography. Grafting synthesis can be used to obtain stable modified silica gel surfaces. In this work, silica gel (10 nm of pore diameter and surface area of 320 m 2 g -1 ) was chemically modified with 3-chloropropyltrimethoxysilane or 3-aminopropyltrimethoxysilane and reacted with aniline, p-anisidine, benzylamine and 3-phenylpropylchloride in order to yield aromatic groups immobilized on the silica gel surface. Infrared spectroscopy was utilized for characterization of the aromatic groups grafted on the silica gel surface, using a quartz cell. The solids were heated at several temperatures in high vacuum and the infrared band areas of the organic groups were used to evaluate the thermal stability.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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FTIR thermal analysis on organofunctionalized silica gel

Foschiera

Pizzolato

Benvenutti

2001

J. Braz. Chem. Soc.

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“…Um método bastante utilizado na imobilização de grupos orgâ-nicos sobre sílica é o enxerto 1,[4][5][6] . Nesse método é comum usar-se um organosilano de fórmula R-Si(OCH 3 ) 3 , sendo R um grupo orgânico alifático, aromático ou ainda cadeia orgânica contendo outros grupos funcionais.…”

Section: Introductionunclassified

Xerogel p-anisidinapropilsílica: estudo da estabilidade térmica e da resistência à lixiviação com solventes

Franken¹,

Santos²,

Caramão³

et al. 2002

Quím. Nova

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“…[11][12][13] The use of chemically modified silica appears as an interesting alternative to solve this problem. Such materials have demonstrated utility in several applications [14][15][16] , due to their interesting and well-defined characteristics, such as: particle size, porosity, high surface area and high mechanical, chemical and thermal stability, as well as a low tendency to swell in the presence of solvents. 17,18 Moreover, previous work has shown the capacity of these modified silicas to adsorb toxic metals with high selectivity.…”

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confidence: 99%

Optimization of Metal Extraction Conditions by Modified Silicas Using a Factorial Design

et al. 1999

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The extraction properties and the selectivity of silica modified with zirconium phosphate (SiZrP) and propylimidazol (SiIm) for cadmium, lead and mercury were evaluated. The optimization of these parameters was carried out by a complete 2 3 factorial design (consisting of two levels and three factors), using the intensity of energy dispersive X-ray fluorescence (EDXRF) as response factor. SiZrP adsorbed the three metallic species, while SiIm adsorbed mercury selectively. This difference was assigned to the particular adsorption process involved in each instance. The interaction factor between pH and silica type was significant, suggesting that an unidimensional study could not have optimized the system. Using small amounts of the adsorbent matrices (0.2 g) resulted in better sensitivity, as well as an important saving of chemicals.

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Use of Chemically Modified Silica with β-Diketoamine Groups for Separation of α-Lactoalbumin from Bovine Milk Whey by Affinity Chromatography

Cited by 23 publications

References 2 publications

FTIR thermal analysis on organofunctionalized silica gel

FTIR thermal analysis on organofunctionalized silica gel

Xerogel p-anisidinapropilsílica: estudo da estabilidade térmica e da resistência à lixiviação com solventes

Optimization of Metal Extraction Conditions by Modified Silicas Using a Factorial Design

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