This article explores the feasibility of producing biohydrogen from microalgae following their use in wastewater purification from food industry facilities and the removal of high levels of carbon dioxide (CO2) from the air. The authors investigated various methods for disrupting the cell membranes of microalgae and their impact on biohydrogen yield. The microalgae biomass obtained after wastewater and air purification underwent pre-treatment using physicochemical and chemical methods, including microwave radiation, acid treatment, and thermal treatment. The highest hydrogen production occurred during thermal and acid treatments of biomass with the addition of starch (44.24 mL/L of suspension). The use of microwave radiation for processing did not yield significant results. A comparison of the biohydrogen values obtained from untreated and treated biomass revealed that treatment enhances biohydrogen yield.