The experiments aimed to verify the use of a focused ion beam (FIB) to create structures analogous to the natural surface and then use the two-step imprinting method to prepare replicas of the surface created by FIB. The surface of the petal leaves of pansy (Viola x wittrockiana) was selected as a natural surface pattern. The TESCAN AMBER FIB-SEM microscope was used for the preparation of an analogous surface. The molds for the replication process were made of President Light Body elastomer. PVB, PVA, CMC, PCL, EP were used for the preparation of polymer replicas. Scanning electron microscope was used to evaluate the quality of the prepared polymer replicas. The dimensions of the replicated convex and concave structures were 25 x 20 µm. The polymers showed a different ability to copy the structure in detail. For PVA, CMC, and PCL, surface structuring at the nanometer level was described in this study. Due to the dimensions of the cones of convex and concave structures, the color was observed on all samples.