Use of collagen and auricular cartilage in bioengineering: scaffolds for tissue regeneration

Massimino, Lívia Contini; Martins, Virgínia da Conceição Amaro; Vulcani, Valcinir Aloísio Scalla; Lucas-Oliveira, Éverton; Andreeta, Mariane Barsi; Bonagamba, Tito J.; Klingbeil, María Fátima Guarizo; Mathor, Mônica Beatriz; Plepis, Ana Maria de Guzzi

doi:10.1007/s10561-020-09861-0

Cited by 14 publications

(16 citation statements)

References 42 publications

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“…Interestingly, for all Coll bioscaffolds, the sizes of the smaller pores (20–80 μm) were about 15% higher, and the larger pores (100–350 μm) were about less than 1% compared to the Coll-free scaffold. The overall pore size and porosity in the range of 20–100 μm and 53–79% are suitable for skin TE or neovascularisation, as suggested by other authors. , In general, the pore size values obtained with the micro-CT were lower than those obtained with the FE-SEM . A possible reason for this could be that the assessment of 3D pore size in micro-CT is based on a sphere fitting algorithm.…”

Section: Resultsmentioning

confidence: 62%

3D-Printed Collagen–Nanocellulose Hybrid Bioscaffolds with Tailored Properties for Tissue Engineering Applications

Dobaj Štiglic,

Lackner,

Nagaraj

et al. 2023

ACS Appl. Bio Mater.

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Hybrid collagen (Coll) bioscaffolds have emerged as a promising solution for tissue engineering (TE) and regenerative medicine. These innovative bioscaffolds combine the beneficial properties of Coll, an important structural protein of the extracellular matrix, with various other biomaterials to create platforms for long-term cell growth and tissue formation. The integration or cross-linking of Coll with other biomaterials increases mechanical strength and stability and introduces tailored biochemical and physical factors that mimic the natural tissue microenvironment. This work reports on the fabrication of chemically cross-linked hybrid bioscaffolds with enhanced properties from the combination of Coll, nanofibrillated cellulose (NFC), carboxymethylcellulose (CMC), and citric acid (CA). The bioscaffolds were prepared by 3D printing ink containing Coll-NFC-CMC-CA followed by freeze-drying, dehydrothermal treatment, and neutralization. Cross-linking through the formation of ester bonds between the polymers and CA in the bioscaffolds was achieved by exposing the bioscaffolds to elevated temperatures in the dry state. The morphology, pores/porosity, chemical composition, structure, thermal behavior, swelling, degradation, and mechanical properties of the bioscaffolds in the dry and wet states were investigated as a function of Coll concentration. The bioscaffolds showed no cytotoxicity to MG-63 human bone osteosarcoma cells as tested by different assays measuring different end points. Overall, the presented hybrid Coll bioscaffolds offer a unique combination of biocompatibility, stability, and structural support, making them valuable tools for TE.

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Section: Resultsmentioning

confidence: 62%

3D-Printed Collagen–Nanocellulose Hybrid Bioscaffolds with Tailored Properties for Tissue Engineering Applications

Dobaj Štiglic,

Lackner,

Nagaraj

et al. 2023

ACS Appl. Bio Mater.

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“…The three membranes used in the present study belong to the latter category. Correlating the histological and morphological characteristics of the tissue repair process, the results of this study showed that the xenogenous collagen, elastin, and chitosan membranes used were biocompatible and promoted the proliferation of osteogenic cells at the site of injury in the nasal bone of rats [ 23 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…The collagen matrix was derived from bovine intestinal serosa and the elastin matrix from bovine auricular cartilage. The manufacturing process of matrices and their characterizations have been described in detail in previously published studies [ 16 , 27 , 28 , 29 , 30 , 31 , 32 ]. In summary, the bovine serosa and auricular cartilage were washed exhaustively in 0.9% saline (NaCl) and distilled water.…”

Section: Methodsmentioning

confidence: 99%

In Vivo Study of Nasal Bone Reconstruction with Collagen, Elastin and Chitosan Membranes in Abstainer and Alcoholic Rats

et al. 2022

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The aim of the present study was to evaluate the use of collagen, elastin, or chitosan biomaterial for bone reconstruction in rats submitted or not to experimental alcoholism. Wistar male rats were divided into eight groups, submitted to chronic alcohol ingestion (G5 to G8) or not (G1 to G4). Nasal bone defects were filled with clot in animals of G1 and G5 and with collagen, elastin, and chitosan grafts in G2/G6, G3/G7, and G4/G8, respectively. Six weeks after, all specimens underwent radiographic, tomographic, and microscopic evaluations. Bone mineral density was lower in the defect area in alcoholic animals compared to the abstainer animals. Bone neoformation was greater in the abstainer groups receiving the elastin membrane and in abstainer and alcoholic rats receiving the chitosan membrane (15.78 ± 1.19, 27.81 ± 0.91, 47.29 ± 0.97, 42.69 ± 1.52, 13.81 ± 1.60, 18.59 ± 1.37, 16.54 ± 0.89, and 37.06 ± 1.17 in G1 to G8, respectively). In conclusion, osteogenesis and bone density were more expressive after the application of the elastin matrix in abstainer animals and of the chitosan matrix in both abstainer and alcoholic animals. Chronic alcohol ingestion resulted in lower bone formation and greater formation of fibrous connective tissue.

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“…The manufacturing process of elastin and collagen matrices and their characterizations have been described in greater detail in previously published studies [7,19,27,[40][41][42]. The elastin matrices used were prepared by the Biochemistry and Biomaterials Group of the São Carlos Institute of Chemistry (University of São Paulo-USP, Brazil) by adapting the methodology used to obtain collagen [7,8,14].…”

Section: Elastin Matrix Derived From Bovine Auricular Cartilagementioning

confidence: 99%

In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

et al. 2021

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Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, n = 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, n = 10), defects filled with blood clot; E24/37 Group (E24/37, n = 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, n = 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25).

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Use of collagen and auricular cartilage in bioengineering: scaffolds for tissue regeneration

Cited by 14 publications

References 42 publications

3D-Printed Collagen–Nanocellulose Hybrid Bioscaffolds with Tailored Properties for Tissue Engineering Applications

3D-Printed Collagen–Nanocellulose Hybrid Bioscaffolds with Tailored Properties for Tissue Engineering Applications

In Vivo Study of Nasal Bone Reconstruction with Collagen, Elastin and Chitosan Membranes in Abstainer and Alcoholic Rats

In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

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