Use of Comparative Proteomics to Identify Key Proteins Related to Hepatic Lipid Metabolism in Broiler Chickens: Evidence Accounting for Differential Fat Deposition Between Strains

Abstract

In order to investigate differences in fat metabolism during embryonic development, a comparative proteomics strategy was employed using Arbor Acres (AA) and San Huang (SH) broiler chickens with different growth and fat deposition characteristics. These birds were floor-reared and fed identical diets, and embryonic livers were collected from AA and SH chicken embryos on days 9, 14 and 19 of incubation and hatching. Proteins were extracted and fractionated by two-dimensional electrophoresis (2-DE), Neuhoff's colloidal Coomassie Blue G-250 staining was carried out, and stained gels were scanned and analyzed using PDQuest7.3 software (Bio-Rad). In-gel trypsin digestion of the differential protein spots and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) were subsequently assessed. Peptide mass fingerprinting of the differentially expressed proteins was performed using the server from MASCOT or either Prospector or ProFound, and 37 proteins were successfully identified. In the present study, embryo and liver weights showed a trend toward enhanced growth during embryonic development. Of the 37 identified differential proteins, phosphoenolpyruvate carboxykinase (PEPCK), apolipoprotein A-I (Apo A-I), fatty acid-binding protein (L-FABP) and 3-hydroxy-3-methylglutaryl-Coenzyme A synthase (HMG-CoA synthase) were up-regulated in SH chickens to a greater extent than they were in AA chickens. These observations suggest that the lipid metabolic proteins and enzymes are inherent characteristics that contribute to the apparent differences in fat deposition between the two strains.

Relationship between liver and low rumen pH in goat

“…Mass spectrometry fingerprinting data searches were performed using the search engines ProFound and MSfit (http://www.matrixscience.com) against the NCBInr database in the taxa of Ruminant (goat or cattle) with the parameter sets of trypsin digestion, 2 missed cleavages, complete modification of iodoacetamide (Cys), partial modification of methionine oxidation, protein mass ± 20% of the observed protein mass, pI = ±1 of observed pI, and a mass tolerance for monoisotopic data of 100 ppm. Protein identification was assigned when the following criteria were met: at least 4 matching peptides and >20% sequence coverage (Huang et al, 2010).…”

Dehydroepiandrosterone Reduced Lipid Droplet Accumulation via Inhibiting Cell Proliferation and Improving Mitochondrial Function in Primary Chicken Hepatocytes

“…Unlike mammalian species, the liver is the most important organ of fat metabolism in poultry (Griffin et al 1992). Our previous study found that DHEA treatment reduced fat deposition in broiler chicken or chicken embryos (Tang et al 2007, Zhao et al 2007, Huang et al 2010. In addition, we found that DHEA accelerates lipid catabolism by direct regulation of hepatic gene expression, and this action mainly achieved by activation of cAMP/PKA signaling pathway in primary chicken hepatocytes (Tang et al 2009a, Tang et al 2009b.…”

Molecular Differences in Hepatic Metabolism between AA Broiler and Big Bone Chickens: A Proteomic Study

“…Previous studies demonstrated differential lipid metabolism between fat and lean broiler breeds [ 5 , 9 , 27 ]. Specifically, this study found that AA broilers had enhanced levels of fatty acid-binding protein (FABP7, spot 27 and 48), enzyme HMG-CoA synthase 1 (HMGCS1, spot 42, 111 and 112), phosphatidylinositol transfer protein (PITPNB, spot 116) and lipid binding protein SEC14-Like 2 (SEC14L3, spot 114).…”

“…The Arbor Acres (AA) broiler is a well-known commercial breed in the poultry industry. It is featured by a large size, rapid-growth rate, high feed-conversion rate and strong adaptability, but possesses less favorable meat quality and excessive abdominal fat [ 9 ]. Gene expression analysis demonstrated that the AA breed had dysregulated lipid metabolism and other cellular pathways compared to a Chinese breed with high meat quality [ 10 ].…”