Use of cyclodextrins for the enantioselective separation of ergot alkaloids by capillary zone electrophoresis

Abstract

Ergot alkaloid enantiomer derivatives were resolved using capillary zone electrophoresis. The effect of cyclodextrins, added to the background electrolyte, on the migration time and the resolution was studied. Good separation for epimeric ergot alkaloid derivatives was also obtained using phosphate buffer at pH 2.5. Separation was improved by supplementing the background electrolyte with 30 mM of gamma-cyclodextrin. Good resolution of racemic ergot alkaloid derivatives in their enantiomers was achieved in a background electrolyte containing either beta-cyclodextrin or its derivative, or gamma-cyclodextrin.

A basic tool for risk assessment: A new method for the analysis of ergot alkaloids in rye and selected rye products

“…For the determination of ergot alkaloids in products of plant origin, a variety of analytical techniques such as colorimetric analysis with van Urk's reagent [2 -4], thin layer chromatography [13 -15], immunoassays [16 -18], CZE [19,20], HPLC coupled with fluorimetric detection (HPLC-FLD) [6, 21 -28], and LC coupled with MS/MS [26, 29 -32] has been reported.…”

The use of cyclodextrins as chiral selectors

“…For example the enantiomers of the ergot alkaloids isolysergic acid, terguride, meluol, nicergoline, and lisuride were se-parated using 7-cyclodextrin in a 100 mM phosphate buffer at pH 2.5 [74]. For example the enantiomers of the ergot alkaloids isolysergic acid, terguride, meluol, nicergoline, and lisuride were se-parated using 7-cyclodextrin in a 100 mM phosphate buffer at pH 2.5 [74].…”

Capillary electrophoresis of natural products

“…Also, selectivity differences and peak crossovers were observed with buffer concentration changes from 15 mM to 55 mM. Fanali et al [34] used different cyclodextrins for the enantioselective separation of ergot alkaloids. Good resolution of racemic mixtures was achieved using both pand y-cyclodextrins.…”