In addition to lasalocid, an oligoether coccidiostatic compound, other compounds are synthesized by Streptomyces lasaliensis. Mutants producing either of two antibiotics, lasalocid A or quinomycin A (an antibiotic of quinoxaline character), were obtained by natural selection and by mutagenesis. Methods of isolation, purification and estimation of both compounds were established.
Ergot alkaloid enantiomer derivatives were resolved using capillary zone electrophoresis. The effect of cyclodextrins, added to the background electrolyte, on the migration time and the resolution was studied. Good separation for epimeric ergot alkaloid derivatives was also obtained using phosphate buffer at pH 2.5. Separation was improved by supplementing the background electrolyte with 30 mM of gamma-cyclodextrin. Good resolution of racemic ergot alkaloid derivatives in their enantiomers was achieved in a background electrolyte containing either beta-cyclodextrin or its derivative, or gamma-cyclodextrin.
Using the Koenigs-Knorr method two isomeric monohydroxynaphthoquinones were glucosidized, i.e. 2-hydroxy-1,4-naphthoquinone (lawson, Ia) and 5-hydroxy-1,4-naphthoquinone (juglone, IIa). The structure of the corresponding glucosides Ib and IIb was proved by 1H NMR, mass, UV, VIS and IR spectra.
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