Monitoring Surface Soil Moisture (SSM) and Root Zone Soil Moisture (RZSM) dynamics at the regional scale is of fundamental importance to many hydrological and ecological studies. This need becomes even more critical in arid and semi-arid regions, where there are a lack of in situ observations. In this regard, satellite-based Soil Moisture (SM) data is promising due to the temporal resolution of acquisitions and the spatial coverage of observations. Satellite-based SM products are only able to estimate moisture from the soil top layer; however, linking SSM with RZSM would provide valuable information on land surface-atmosphere interactions. In the present study, satellite-based SSM data from Soil Moisture and Ocean Salinity (SMOS), Advanced Microwave Scanning Radiometer 2 (AMSR2), and Soil Moisture Active Passive (SMAP) are first compared with the few available SM in situ observations, and are then coupled with the Soil Moisture Analytical Relationship (SMAR) model to estimate RZSM in Iran. The comparison between in situ SM observations and satellite data showed that the SMAP satellite products provide more accurate description of SSM with an average correlation coefficient (R) of 0.55, root-mean-square error (RMSE) of 0.078 m 3 m −3 and a Bias of 0.033 m 3 m −3 . Thereafter, the SMAP satellite products were coupled with SMAR model, providing a description of the RZSM with performances that are strongly influenced by the misalignment between point and pixel processes measured in the preliminary comparison of SSM data.Hydrology 2019, 6, 44 2 of 13 emissions are widely investigated in many researches due to their potentials for monitoring SM in all temporal and meteorological conditions and the infiltration ability of microwave emissions in sparse vegetation covers. This method functions based on the high level of difference between the soil and water dielectric constants. Solar illumination and cloud cover do not influence microwave remote sensing technique, and its longer wavelengths are not susceptible to atmospheric scattering. Thus, it was considered as the most effective method in remote sensing of SM [11,13]. In recent years, the surface-reflected Global Navigation Satellite System (GNSS) signals have also been evaluated for SM estimations, which applies a different source of signals from the active/passive microwave sensors to observe the Earth's surface [14]. Moreover, the Advanced Scatterometer (ASCAT), which is an active microwave remote sensing instrument, provides global SM data sets derived from the backscatter measurements [15,16].SM products obtained from active/passive microwave remotely-sensed data have been applied in wide spectra of contexts [17][18][19][20][21][22][23][24][25][26]. However, SM data derived from most of the satellite sources provide the near surface moisture that needs to be converted in Root Zone Soil Moisture (RZSM) estimations [27,28]. Recently, high-resolution observations of RZSM have become available through the NASA Airborne Microwave Observatory of Subcanopy and Subsurfac...