Use of Echinostoma spp. in studies on chemotherapy and metabolic profiling.

Saric, Jasmina; Li, Jia V.; Wang, Yulan; Holmes, Elaine; Utzinger, Jürg; Keiser, Jennifer

doi:10.1007/978-0-387-09577-6_13

Cited by 2 publications

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References 83 publications

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“…In vivo studies in Echinostoma spp. infected mice, with various test compounds such as the benzimidazoles, praziquantel, the artemisinins or tribendimidine against echinostomes in mice have been summarized recently (Saric et al 2009). For example, E. caproni-infected mice were cured following a single oral dose of 700 mg/kg artesunate .…”

Section: E Caproni In Vivo Assaymentioning

confidence: 99%

In vitroandin vivotrematode models for chemotherapeutic studies

2009

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Schistosomiasis and food-borne trematodiases are chronic parasitic diseases affecting millions of people mostly in the developing world. Additional drugs should be developed as only few drugs are available for treatment and drug resistance might emerge. In vitro and in vivo whole parasite screens represent essential components of the trematodicidal drug discovery cascade. This review describes the current state-of-the-art of in vitro and in vivo screening systems of the blood fluke Schistosoma mansoni, the liver fluke Fasciola hepatica and the intestinal fluke Echinostoma caproni. Examples of in vitro and in vivo evaluation of compounds for activity are presented. To boost the discovery pipeline for these diseases there is a need to develop validated, robust high-throughput in vitro systems with simple readouts.

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Section: E Caproni In Vivo Assaymentioning

confidence: 99%

In vitroandin vivotrematode models for chemotherapeutic studies

2009

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“…Both in vitro and in vivo studies with E. caproni can be conducted with relative ease, as mice can be infected with 30 to almost 300 metacercariae and an adult infection is established as soon as 2 weeks post-infection [6]. E. caproni can also be used as a pre-screening tool for potential trematocidal compounds, though caution must be applied, as E. caproni has different feeding habits and niches in the definitive host as compared to other trematodes [7]. …”

Section: Introductionmentioning

confidence: 99%

Development of an in vitro drug sensitivity assay based on newly excysted larvae of Echinostoma caproni

2013

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BackgroundEchinostomiasis is one of the major food-borne trematodiases and the species Echinostoma caproni serves as a useful model for trematocidal drug discovery. The current in vitro drug sensitivity assay uses adult E. caproni worms that are incubated with candidate drugs and scored microscopically for viability at 72 hrs. The aim of this study was to investigate the use of newly excysted larvae (NEL) of E. caproni for in vitro drug testing, which would be faster, more cost effective and more ethical compared to adult worm assays.MethodsLarvae were obtained by collecting metacercariae from snails and triggering their excystation using the trypsin-bile salt excystation method. Studies concerning various parameters of this chemical transformation process as well as appropriate NEL culturing conditions were carried out and findings evaluated. NEL and adult worms were incubated with praziquantel, tribendimidine, albendazole and quinine and evaluated microscopically 72 hrs post-incubation. In addition, the colorimetric markers resazurin, CellTiter-Glo® and Vybrant® were tested as an alternative assay read-out method.ResultsThe chemical excystation method successfully induced E. caproni metacercariae to excyst at a rate of about 20-60%. NEL remained viable in culture medium for 5–7 days. The results of an in vitro drug assay using NEL mirrored the results of an assay using adult worms incubated with the same drugs. None of the markers could reliably produce signals proportional to NEL viability or cytotoxicity without significant complications.ConclusionNEL are adequate for in vitro drug testing. Challenges remain in further improving the excystation yield and the practicability of the assay setup. Resolving these issues could also improve read-outs using colorimetric markers. Using NEL is in alignment with the 3 R rules of the ethical use of laboratory animals and can greatly increase the rate and affordability with which drugs are screened in vitro against this intestinal trematode.

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Use of Echinostoma spp. in studies on chemotherapy and metabolic profiling.

Cited by 2 publications

References 83 publications

In vitroandin vivotrematode models for chemotherapeutic studies

In vitroandin vivotrematode models for chemotherapeutic studies

Development of an in vitro drug sensitivity assay based on newly excysted larvae of Echinostoma caproni

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