Use of exogenous specialised bacteria in the biological detoxification of a dump site-polychlorobiphenyl-contaminated soil in slurry phase conditions

Fava, Fabio; Bertin, Lorenzo

doi:10.1002/(sici)1097-0290(19990720)64:2<240::aid-bit13>3.0.co;2-f

Cited by 37 publications

(22 citation statements)

References 36 publications

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“…Besides determining the actual organopollutant depletion, it is important to monitor the eventual toxicity reduction to assess the efficacy of a bioremediation approach (Fava and Bertin, 1999;Horvath et al, 1997). In the present study, both fungi proved to be able to significantly reduce soil toxicity, albeit the detoxification extent depended both on the fungus and the test organism.…”

Section: Discussionmentioning

confidence: 64%

“…The organic carbon, total nitrogen, and total phosphorous contents were 38.6, 1.2, and 4.8 g/kg, respectively, while those of sand, silt, and clay were 19.36, 77.14, and 3.50% (w/w), respectively (Berselli et al, 2004). The concentration of indigenous heterotrophic bacteria of the soil, determined by a plate-counting technique (Fava and Bertin, 1999), was $2 Â 10 8 CFU/g. The soil was heavily contaminated with substituted mono-and polycyclic aromatic hydrocarbons; Table I reports contaminants detected in the soil and their concentrations.…”

Section: Soils and Microorganismsmentioning

confidence: 98%

“…The flasks were closed with a plastic cover with holes and stored in a dark chamber at room temperature for 14 days. The toxic effect was determined by calculating the percent mortality of the adults obtained for each soil concentration and for control microcosms as reported by Fava and Bertin (1999).…”

Section: Ecotoxicological Analysesmentioning

confidence: 99%

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Degradation of aromatic hydrocarbons by white‐rot fungi in a historically contaminated soil

D’Annibale

Ricci²,

Leonardi

et al. 2005

Biotech & Bioengineering

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Phanerochaete chrysosporium NRRL 6361 and Pleurotus pulmonarius CBS 664.97 were tested for their ability to grow under nonsterile conditions and to degrade various aromatic hydrocarbons in an aged contaminated soil that also contained high concentrations of heavy metals. After 24 days fungal incubation, carbon-CO2 liberated, an indicator of microbial activity, reached a plateau. At the end of the incubation time (30 days), fungal colonization was clearly visible and was confirmed by ergosterol and cell organic carbon determinations. In spite of unfavorable pH (around 7.4) and the presence of heavy metals, both fungi produced Mn-peroxidase activity. In contrast, laccase and aryl-alcohol oxidase were detected only in the soil treated with P. pulmonarius CBS 664.97 and lignin-peroxidase in that with P. chrysosporium NRRL 6361. No lignin-modifying enzyme activities were present in non-inoculated soil incubated for 30 days (control microcosm). Regardless of the fungus employed, a total removal of naphtalene, tetrachlorobenzene, and dichloroaniline isomers, diphenylether and N-phenyl-1-naphtalenamine, was observed. Significant release of chloride ions was also observed in fungal-treated soil, in comparison with that recorded in the control microcosm. Both fungi led to a significant decrease in soil toxicity, as assessed using two different soil contact assays, including the Lepidium sativum L. germination test and the Collembola mortality test.

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Section: Discussionmentioning

confidence: 64%

Section: Soils and Microorganismsmentioning

confidence: 98%

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Degradation of aromatic hydrocarbons by white‐rot fungi in a historically contaminated soil

D’Annibale

Ricci²,

Leonardi

et al. 2005

Biotech & Bioengineering

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“…Polychlorinated biphenyls (PCBs) are xenobiotic compounds of great concern and are spread widely throughout the environment. PCBs occurring in soils can be partially biodegraded by aerobic consortia of PCB-cometabolizing bacteria and chlorobenzoic acid (CBA)-mineralizing bacteria (Focht, 1995), and the process can be enhanced through soil supplementation with oxygen, biphenyl (Robinson and Lenn, 1994), and exogenous specialized bacteria (Fava and Bertin, 1999), and by treatment conditions able to provide a high degree of soil mixing and homogeneity (Fava et al, 2000). However, the bioremediation of PCB-contaminated soils is very often adversely affected by the low bioavailability of PCBs, which are hydrophobic and tend to adsorb strongly onto soil organic matter, thus becoming poorly available in the soil water phase, where the PCB-and CBAdegrading microorganisms are mainly located (Providenti et al, 1993;Volkering et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Methyl‐β‐cyclodextrin‐enhanced solubilization and aerobic biodegradation of polychlorinated biphenyls in two aged‐contaminated soils

Fava¹,

Bertin²,

Fedi³

et al. 2002

Biotech & Bioengineering

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The bioremediation of aged polychlorinated biphenyl (PCB)-contaminated soils is adversely affected by the low bioavailability of the pollutants. Randomly methylated-beta-cyclodextrins (RAMEB) were tested as a potential PCB-bioavailability-enhancing agent in the aerobic treatment of two aged-contaminated soils. The soils, contaminated by about 890 and 8500 mg/kg of Aroclor 1260 PCBs, were amended with biphenyl (4 g/kg), inorganic nutrients (to adjust their C:N ratio to 20:1), and variable amounts of RAMEB (0%, 0.5%, or 1.0% [w/w]) and treated in both aerobic 3-L solid-phase reactors and 1.5-L packed-bed loop reactors for 6 months. Notably, significant enhancement of the PCB biodegradation and dechlorination, along with a detectable depletion of the initial soil ecotoxicity, were generally observed in the RAMEB-treated reactors of both soils. RAMEB effects were different in the two soils, depending upon the treatment conditions employed, and generally increased proportionally with the concentration at which RAMEB was applied. RAMEB, which was slowly metabolized by the soil's aerobic microorganisms, was found to markedly enhance the occurrence of the indigenous aerobic, cultivable biphenyl-growing bacteria harboring genes homologous to those of two highly specialized PCB degraders (i.e., bphABC genes of Pseudomonas pseudoalcaligenes KF707 and bphA1A2A3A4BC1 genes of Rhodococcus globerulus P6) and chlorobenzoic acid-degrading bacteria as well as the occurrence of PCBs in the water phase of the soil reactors. These findings indicate that RAMEB enhanced the aerobic bioremediation of the two soils by increasing the bioavailability of PCBs and the occurrence of specialized bacteria in the soil reactors.

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“…However, many aerobic bacterial strains like Pseudomonas , Burkholderia , Rhodococcus display degradative activity to lower and highly chlorinated biphenyls (Piper 2005; Egorova et al 2011; De et al 2006; Hatamian-Zarmi et al 2009; Petrić et al 2011). Fava and Bertin (1999) have reported that exogeneous PCB and CBA degrading bacteria can be used in slurry phase in presence of biphenyl and oxygen for effective bioremediation of PCB contaminated soil. The following bacteria showed encouraging results: a mixture of gfp-transformed strains in soil microcosm ( Pseudomonas sp.…”

Section: Bioaugmentation Of Aerobic Organismsmentioning

confidence: 99%

Advances and perspective in bioremediation of polychlorinated biphenyl-contaminated soils

Sharma

Gautam

Nanekar

et al. 2017

Environ Sci Pollut Res

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In recent years, microbial degradation and bioremediation approaches of polychlorinated biphenyls (PCBs) have been studied extensively considering their toxicity, carcinogenicity and persistency potential in the environment. In this direction, different catabolic enzymes have been identified and reported for biodegradation of different PCB congeners along with optimization of biological processes. A genome analysis of PCB-degrading bacteria has led in an improved understanding of their metabolic potential and adaptation to stressful conditions. However, many stones in this area are left unturned. For example, the role and diversity of uncultivable microbes in PCB degradation are still not fully understood. Improved knowledge and understanding on this front will open up new avenues for improved bioremediation technologies which will bring economic, environmental and societal benefits. This article highlights on recent advances in bioremediation of PCBs in soil. It is demonstrated that bioremediation is the most effective and innovative technology which includes biostimulation, bioaugmentation, phytoremediation and rhizoremediation and acts as a model solution for pollution abatement. More recently, transgenic plants and genetically modified microorganisms have proved to be revolutionary in the bioremediation of PCBs. Additionally, other important aspects such as pretreatment using chemical/physical agents for enhanced biodegradation are also addressed. Efforts have been made to identify challenges, research gaps and necessary approaches which in future, can be harnessed for successful use of bioremediation under field conditions. Emphases have been given on the quality/efficiency of bioremediation technology and its related cost which determines its ultimate acceptability.

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Use of exogenous specialised bacteria in the biological detoxification of a dump site-polychlorobiphenyl-contaminated soil in slurry phase conditions

Cited by 37 publications

References 36 publications

Degradation of aromatic hydrocarbons by white‐rot fungi in a historically contaminated soil

Degradation of aromatic hydrocarbons by white‐rot fungi in a historically contaminated soil

Methyl‐β‐cyclodextrin‐enhanced solubilization and aerobic biodegradation of polychlorinated biphenyls in two aged‐contaminated soils

Advances and perspective in bioremediation of polychlorinated biphenyl-contaminated soils

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