Use of Fluorescence Foldscope as an Effective Tool for Detection of Biofilm Formation in Pseudomonas aeruginosa

Deshamukhya, Chandrayee; Das, Bhaskar Jyoti; Chetri, Shiela; Paul, Deepjyoti; Chanda, Debadatta Dhar; Banerjee, Tuhina; Bhattacharjee, Amitabha

doi:10.4103/ijmm.ijmm_20_118

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Biofilm-forming bacteria are responsible for a wide range of chronic disorders that are generally difficult to cure (Deshamukhya et al, 2020). Deshamukhya et al, 2020, on the other hand, employed a fluorescent foldscope with blue light and filters to detect biofilm development in P. aeruginosa strains. Foldscope is used to detect and diagnose a wide spectrum of novel fish infections in freshwater that are known to be the causal organisms in fish.…”

Section: Diagnosismentioning

confidence: 99%

“…However, Prusty & Kumar, 2021, developed a new screening and drug susceptibility study on Candida albicans , a prominent fungal infection in human that causes candidiasis, utilizing foldscope microscopy to examine morphological changes and snap images. Biofilm‐forming bacteria are responsible for a wide range of chronic disorders that are generally difficult to cure (Deshamukhya et al, 2020). Deshamukhya et al, 2020, on the other hand, employed a fluorescent foldscope with blue light and filters to detect biofilm development in P. aeruginosa strains.…”

Section: Alternative To Conventional Microscopesmentioning

confidence: 99%

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Foldscope microscope, an inexpensive alternative tool to conventional microscopy—Applications in research and education: A review

Ganesan

Christyraj

Venkatachalam

et al. 2022

Microscopy Res & Technique

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Microscope is a device used for the visualization of tiny objects which are not visible to the naked eye. Traditional microscopes have been crucial for the advancement of contemporary science and medicine. Recent advancements in the field of microscopy have fueled its exponential growth rate. However, due to their expensive cost and complicated structure, modern microscopes remain inaccessible to the majority of the public. Nonetheless, the foldscope paper microscope has made it possible for anyone to explore and understand the world of microbes and organisms. In this review, we have listed foldscope-based research projects in various domains, as well as their key properties when compared to traditional research microscopes. In addition, we have briefly explored the impact of a foldscope microscope on public health, clinical diagnostics, forensic science, agriculture, basic science, developmental biology, and education. Moreover, the major drawbacks of paper microscopes and the current steps being taken to upgrade foldscope and its features are discussed in this review. Finally, we have concluded with our perspective that the microscope may be updated to imitate the advancement of a conventional microscope. Research Highlights• The foldscope, a low-cost instrument for studying the microscopic world.• Foldscope applications were compared to conventional microscopes in many sectors.• The foldscope microscope's existing limitations and potential prospects are highlighted.

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Section: Diagnosismentioning

confidence: 99%

Section: Alternative To Conventional Microscopesmentioning

confidence: 99%

Foldscope microscope, an inexpensive alternative tool to conventional microscopy—Applications in research and education: A review

Ganesan

Christyraj

Venkatachalam

et al. 2022

Microscopy Res & Technique

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“…Fluorescence microscopy is an effective technique for real-time detection, and it is possible to use with simple mounts preparations [26]. Microorganisms (either bacteria or eukaryotic cells) are stained with a fluorescent dye, such as Syto-9, without the need for fixation, the staining procedure is simple as it involves adding the dye to the liquid sample and a 15-minute incubation at room temperature followed by the microscopic observation [32][33][34][35][36]. The use of Syto-9 presents some advantages, such as it is non-hazardous, has a low-cost application (around $0.05/sample) and can be transported at room temperature.…”

Section: Plos Global Public Healthmentioning

confidence: 99%

Toward co-design of an AI solution for detection of diarrheal pathogens in drinking water within resource-constrained contexts

Hall-Clifford

Arzu

Contreras

et al. 2022

PLOS Glob Public Health

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Despite successes on the Sustainable Development Goals for access to improved water sources and sanitation, many low and middle-income countries (LMICs) continue to struggle with high rates of diarrheal disease. In Guatemala, 98% of water sources are estimated to have E. coli contamination. This project moves toward a novel low-cost approach to bridge the gap between the microbiologic identification of E. coli and the vast impact that this pathogen has on human health within marginalized communities using co-designed community-based tools, low-cost technology, and AI. An agile co-design process was followed with water quality stakeholders, community staff, and local graphic design artists to develop a community water quality education mobile app. A series of alpha- and beta-testers completed interactive demonstration, feedback, and in-depth interview sessions. A microbiology lab in Guatemala developed and piloted field protocols with lay community workers to collect and process water samples. A preliminary artificial intelligence (AI) algorithm was developed to detect the presence of E. coli in images generated from community-derived water samples. The mobile app emerged as a pictorial and audio-driven community-facing tool. The field protocol for water sampling and testing was successfully implemented by lay community workers. Feedback from the community workers indicated both desire and ability to conduct the water sampling and testing protocol under field conditions. However, images derived from the low-cost $2 microscope in field conditions were not of a suitable quality for AI object detection of E. coli, and additional low-cost technologies are being considered. The preliminary AI object detection algorithm from lab-derived images performed at 94% accuracy in identifying E. coli in comparison to the Chromocult gold-standard.

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Role of physicochemical organization of Rhyothemis variegata wing in monitoring bactericidal activity

Paikra

Mishra

2021

Surfaces and Interfaces

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Use of Fluorescence Foldscope as an Effective Tool for Detection of Biofilm Formation in Pseudomonas aeruginosa

Cited by 4 publications

References 15 publications

Foldscope microscope, an inexpensive alternative tool to conventional microscopy—Applications in research and education: A review

Foldscope microscope, an inexpensive alternative tool to conventional microscopy—Applications in research and education: A review

Toward co-design of an AI solution for detection of diarrheal pathogens in drinking water within resource-constrained contexts

Role of physicochemical organization of Rhyothemis variegata wing in monitoring bactericidal activity

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