Use of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars

Chan, Y.L.; Ngan, A.H.W.; King, Nigel M.

doi:10.1016/j.jmbbm.2009.01.006

Cited by 50 publications

(36 citation statements)

References 45 publications

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“…Consequently, it can be assumed that FIB treatment does not have a significant effect on the measured mechanical properties of enamel. The resulting low elastic modulus values were attributed to the dissolution of minerals in the micro-cantilevers by the acid treatment (this etching procedure is necessary to make the prism boundaries visible and locate the milling site correctly) [49]. Moreover, as mentioned earlier, the HAP crystallites differ slightly from synthetic HAP in chemistry and structure, which may also alter their mechanical properties [35].…”

Section: (B) Hierarchical Level 1: Multiple Crystallites (I) Structurmentioning

confidence: 98%

Influence of structural hierarchy on the fracture behaviour of tooth enamel

Yılmaz

Schneider

Swain

2015

Phil. Trans. R. Soc. A.

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Tooth enamel has the critical role of enabling the mastication of food and also of protecting the underlying vital dentin and pulp structure. Unlike most vital tissue, enamel has no ability to repair or remodel and as such has had to develop robust damage tolerance to withstand contact fatigue events throughout the lifetime of a species. To achieve such behaviour, enamel has evolved a complex hierarchical structure that varies slightly between different species. The major component of enamel is apatite in the form of crystallite fibres with a nanometresized diameter that extend from the dentin-enamel junction to the oral surface. These crystallites are bound together by proteins and peptides into a range of hierarchical structures from micrometre diameter prisms to 50-100 µm diameter bundles of prisms known as Hunter-Schreger bands. As a consequence of such complex structural organization, the damage tolerance of enamel increases through various toughening mechanisms in the hierarchy but at the expense of fracture strength. This review critically evaluates the role of hierarchy on the development of the R-curve and the stress-strain behaviour. It attempts to identify and quantify the multiple mechanisms responsible for this behaviour as well as their impact on damage tolerance.

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Section: (B) Hierarchical Level 1: Multiple Crystallites (I) Structurmentioning

confidence: 98%

Influence of structural hierarchy on the fracture behaviour of tooth enamel

Yılmaz

Schneider

Swain

2015

Phil. Trans. R. Soc. A.

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“…The bone structure consists of osteoprogenitor cells, support osteoblasts and osteocytes, remodeling cells -osteoclasts -and a nonmineralized extracellular matrix called the osteoid, composed of type I collagen and non-collagen proteins such as osteonectin, osteocalcin, Morphonetic bone protein (BMP), glycosaminoglycans (GAG) and bone sialoproteins [26][27][28][29].…”

Section: Bone Engineeringmentioning

confidence: 99%

Main considerations on the use of biomembranes in implantology: A review

Prates¹,

Filho²,

Cicarelli³

2017

Dent Oral Craniofac Res

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“…Of these studies, the ability to mechanically test discrete volumes of bone is particularly beneficial for understanding the synergy between constituents while removing the effects of sample geometry (Jimenez-Palomar et al, 2012). Microbeams selected using focused ion beam (FIB) microscopy have been previously employed to understand the mechanical properties of biological materials including teeth (Chan et al, 2009) and bone (Jimenez-Palomar et al, 2012). This work therefore exploits micro-beams from cortical bone but expands on the technique to evaluate the effects of osteoporosis on resultant bone material mechanics.…”

Section: Frontiers In Materials | Mechanics Of Materialsmentioning

confidence: 99%

Mechanical Behavior of Osteoporotic Bone at Sub-Lamellar Length Scales

et al. 2015

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Osteoporosis is a disease known to promote bone fragility but the effect on the mechanical properties of bone material, which is independent of geometric effects, is particularly unclear. To address this problem, micro-beams of osteoporotic bone were prepared using focused ion beam microscopy and mechanically tested in compression using an atomic force microscope while observing them using in situ electron microscopy. This experimental approach was shown to be effective for measuring the subtle changes in the mechanical properties of bone material required to evaluate the effects of osteoporosis. Osteoporotic bone material was found to have lower elastic modulus and increased strain to failure when compared to healthy bone material, while the strength of osteoporotic and healthy bone was similar. Surprisingly, the increased strain to failure for osteoporotic bone material provided enhanced toughness relative to the control samples, suggesting that lowering of bone fragility due to osteoporosis is not defined by material performance. A mechanism is suggested based on these results and previous literature that indicates degradation of the organic material in osteoporosis bone is responsible for resultant mechanical properties.

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Use of focused ion beam milling for investigating the mechanical properties of biological tissues: A study of human primary molars

Cited by 50 publications

References 45 publications

Influence of structural hierarchy on the fracture behaviour of tooth enamel

Influence of structural hierarchy on the fracture behaviour of tooth enamel

Main considerations on the use of biomembranes in implantology: A review

Mechanical Behavior of Osteoporotic Bone at Sub-Lamellar Length Scales

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