Use of Gamma Radiation for the Genetic Improvement of Underutilized Plant Varieties

Riviello-Flores, María de la Luz; Cadena-Íñiguez, Jorge; Ruíz-Posadas, Lucero del Mar; Arévalo-Galarza, Ma. de Lourdes; Castillo‐Juárez, Israel; Soto-Hernández, Ramón Marcos; Castillo-Martínez, C.R.

doi:10.3390/plants11091161

Cited by 40 publications

(24 citation statements)

References 56 publications

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“…For example, 50 Gy gamma radiation produced a sensitizing effect on chloroplasts. Moreover, high Gy doses affect protein synthesis, hormonal balance, enzymatic activity, gas, and water exchange (Riviello-Flores et al, 2022). Nowadays, new variations are needed to develop more nutritious, resistant, and productive varieties (Amri-Tiliouine et al, 2018;Shuryak et al, 2019).…”

Section: Gamma Radiation In Plant Breedingmentioning

confidence: 99%

Obtaining haploid plants by irradiated pollen culture in oil seed crops

Yaman

Sanyürek

2023

Biotech Studies

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Haploid plant production is of great importance to shorten the breeding period in plant breeding programs. Obtaining pure lines in plant growing programs require an intensive work with huge labor and time. Obtaining one hundred percent homozy-gous pure lines is a key point for the improvement and development of new cultivars. Haploid plants with a single set of homozygous chromosomes have become a valua-ble tool in plant breeding. Dihaploid plants that are homozygous at all loci with dou-bling of their chromosomes can be propagated by seed and reach full homozygosity in a single generation. Traditional methods take seven years to reach homozygosity. Dihaploidization methods provide significant advantages in terms of gaining homo-zygosity in a short period of one year and bringing pure lines into agriculture. Anther culture and irradiated pollen technique are among the most widely used techniques in this respect; where physical or chemical agents are used to induce mutated pollen grains and anthers that are subsequently employed to develop dihaploids through in vitro cultures. These techniques are a good source to facilitate gene mapping, cyto-genetic research, and evolutionary studies. Irradiated pollen culture techniques have been applied to many oilseed crops to obtain pure lines. This study highlights some salient features of producing dihaploids using irradiated pollen grains and their maintenance.

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Section: Gamma Radiation In Plant Breedingmentioning

confidence: 99%

Obtaining haploid plants by irradiated pollen culture in oil seed crops

Yaman

Sanyürek

2023

Biotech Studies

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“…The number of seeds tip, and leaf shape. The variables of leaf morphology in this study were based on observations during the research and several studies related to changes in leaf shape due to irradiation treatment (Meilawati et al 2016;Riviello-Flores 2022). As for leaf color, the percentage of each color is calculated based on observations during the research.…”

Section: Accelerated Aging and Gamma Irradiation Treatmentsmentioning

confidence: 99%

The Changes in Seed Germination Capacity, Seedling Growth, and Leaf Morphology of Ficus variegata Blume Influenced by Gamma-Ray Irradiation

Zanzibar¹,

Pramono²,

Yulianti³

et al. 2022

HAYATI J Biosci

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Gamma irradiation treatments have commonly been applied to induce variation in plant genetic improvement programs. The improvement of nyawai (Ficus variegata Blume) is urgently needed mainly to break the seed dormancy, increase seedling growth, and induce variation for selecting desired traits. This research aimed to assess the effect of gamma irradiation doses on seed germination, seedling growth, and leaf morphology of Ficus variegate Blume. This experiment's irradiation doses ranged from 5-240 Gy and were applied to seeds with varying levels of vigor based on the artificial seed aging process. The data displayed a positive relationship between low doses of gamma irradiation and the nyawai seed's endurance. Low irradiation doses, 5 to 30 Gy, are deemed capable of promoting seedling development, leaf retention, and diversity in leaf structure. The higher irradiation doses than 30 Gy decreased seedling growth, leaf number, and seedling survival. In addition, these doses also affect some morphological changes, including seedling height and leaf length and width.

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“…These parameters depend on the dosage, species, varieties, plant tissue (seed, meristem, callus, etc. ), stage of development, and moisture content in the radiation time (Riviello-Flores et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Many authors so far reported a reduction of regenerative capacity and malformation of plant tissues, as well as, tissue destruction due to the high dose of radiation (Chakravarty and Sen, 2001;Yamaguchi, 2018;Andrew-Peter-Leon et al, 2021). Radio sensitivity tests are essential for determining the appropriate radiation dose to induce the highest genetic diversity with the most useful fixed mutations (Songsri et al, 2019;Riviello-Flores et al, 2022). Studies also stated that irradiation with low radiation doses stimulates the plants' vitality, which is beneficial for plant regeneration (Jala and Bodhipadma, 2011;Abubakar et al, 2017;Surakshitha and Soorianathasundaram, 2017;Aisyah et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Molecular, Phenotypic Marker Assays, and Radiosensitivity Tests of Gamma-Irradiated Celosia Argentea

KURUCZ¹,

ANDRE²,

FARI³

et al. 2022

SABRAOJBG

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Random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), and UPOV phenotype markers were used to study the DNA polymorphism in gamma-ray induced morphological mutants of Celosia argentea var. plumosa Hungarian variety ‘Arrabona.‘ In the experiments, the study determined the radio sensitivity and the genetic diversity of gamma radiation of C. argentea var. plumosa ‘Arrabona.‘ Seeds of C. argentea var. plumosa ‘Arrabona‘ were irradiated with gamma rays to increase their genetic diversity. The irradiation doses consisted of 0, 75, 150, 300, 450, and 600 Grays (Gy). The germination percentage, survival rate, and phenotype of irradiated plantlets underwent evaluation in the first (M1) and second (M2) generations. The investigation of genetic diversity used the ISSR and RAPD primers. Based on the results, the first-generation genetic distance increased as the doses increased. But the trend changed considerably through the generation due to the low condition and fertility of the high doses of gamma-irradiated plants. These individuals did not show at the next mutant generation, changing the population gene pool. In addition, open pollination has also changed genetic diversity. The RAPD and ISSR primers proved proper to evaluate the genetic diversity, nonetheless fewer direct connection occurred between the appearance and the used RAPD or ISSR markers. The LD50 dose between 150 and 300 Gray treatments and the radiation between 300450 Gray induced the median growth reduction in the mutant ‘Arrabona‘ population. Based on these results, the study concluded that both UPOV-based phenotyping and molecular marker analysis revealed appropriate for determining genetic divergence, but detecting greater genetic distance resulted in molecular markers.

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Use of Gamma Radiation for the Genetic Improvement of Underutilized Plant Varieties

Cited by 40 publications

References 56 publications

Obtaining haploid plants by irradiated pollen culture in oil seed crops

Obtaining haploid plants by irradiated pollen culture in oil seed crops

The Changes in Seed Germination Capacity, Seedling Growth, and Leaf Morphology of Ficus variegata Blume Influenced by Gamma-Ray Irradiation

Molecular, Phenotypic Marker Assays, and Radiosensitivity Tests of Gamma-Irradiated Celosia Argentea

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