Use of GC×GC for the characterization of odours in forensic applications

Dubois, Lena; O’Sullivan, Gwen; Stefanuto, Pierre‐Hugues; Sandau, Courtney D.; Focant, Jean‐François

doi:10.1016/bs.coac.2021.11.007

Cited by 5 publications

(1 citation statement)

References 66 publications

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“…Furthermore, bidimensional gas chromatography as successfully performed in cadaveric VOC analyses [ 103 , 158 ] could additionally help to increase the correlation between bacteria and volatiles. The problem of compound coelution in unidimensional gas chromatography can be diminished by a better resolution and consequently a higher cadaveric compound diversity in the two-dimensional GCxGC technique [ 103 , 159 ].…”

Section: Discussionmentioning

confidence: 99%

Linking bacteria, volatiles and insects on carrion: the role of temporal and spatial factors regulating inter-kingdom communication via volatiles

et al. 2022

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Multi-kingdom community complexity and the chemically mediated dynamics between bacteria and insects have recently received increased attention in carrion research. However, the strength of these inter-kingdom interactions and the factors that regulate them are poorly studied. We used 75 piglet cadavers across three forest regions to survey the relationship between three actors (epinecrotic bacteria, volatile organic compounds (VOCs) and flies) during the first 4 days of decomposition and the factors that regulate this interdependence. The results showed a dynamic bacterial change during decomposition (temperature–time index) and across the forest management gradient, but not between regions. Similarly, VOC emission was dynamic across a temperature–time index and the forest management gradient but did not differ between regions. However, fly occurrence was dynamic across both space and time. The strong interdependence between the three actors was mainly regulated by the temperature–time index and the study regions, thereby revealing regulation at temporal and spatial scales. Additionally, the actor interdependence was stable across a gradient of forest management intensity. By combining different actors of decomposition, we have expanded our knowledge of the holistic mechanisms regulating carrion community dynamics and inter-kingdom interactions, an important precondition for better describing food web dynamics and entire ecosystem functions.

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Section: Discussionmentioning

confidence: 99%

Linking bacteria, volatiles and insects on carrion: the role of temporal and spatial factors regulating inter-kingdom communication via volatiles

et al. 2022

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Future Aspects of Modern Forensic Tools and Devices

Satish

Phadke

Rawtani

2023

Modern Forensic Tools and Devices

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Applying a risk assessment guided evaluation for verifying comprehensive two-dimensional gas chromatography to analyse complex pharmaceuticals

Schwalb,

Tiemann,

Käfer

et al. 2023

Anal Bioanal Chem

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The reliability of analytical results is critical and indispensable when applied in regulated environments such as the pharmaceutical industry. Therefore, analytical workflows must be validated. However, validation guidelines are often designed for quantitative targeted analysis and rarely apply to qualitative untargeted approaches. In this study, we employ a risk assessment approach to identify critical parameters which might influence the qualitative results derived by online derivatisation — comprehensive two-dimensional gas chromatography coupled to a high-resolution time-of-flight mass spectrometer (GC × GC-HR-ToF–MS) for the analysis of the active pharmaceutical ingredient (API) sodium bituminosulfonate (SBS). To show the complexity and feasibility of such an approach, we focus on investigating three potential risk factors: sample preparation, vapourability, and the thermal stability of sulfonates. Through the individual evaluation of these potential risk factors due to the application of sample preparation approaches and thermal gravimetric analysis (TGA), we demonstrate the high derivatisation efficiency and repeatability of the online derivatisation method and confirm the absence of derivatisation-induced side reactions. In addition, we also show the potential thermal instability of an incompletely derivatised API. To address the limitation of these individual assessments, we applied a holistic evaluation step with negative electrospray ionisation Fourier transform ion cyclotron resonance mass spectrometry (ESI( −) FT-ICR MS) as an orthogonal technique. This confirms that most of the API is detected via the presented GC-based method. Thereby, we demonstrated the practical feasibility of the risk assessment-based approach to ensure the validity of the qualitative data for a complex untargeted method. Graphical Abstract

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Use of GC×GC for the characterization of odours in forensic applications

Cited by 5 publications

References 66 publications

Linking bacteria, volatiles and insects on carrion: the role of temporal and spatial factors regulating inter-kingdom communication via volatiles

Linking bacteria, volatiles and insects on carrion: the role of temporal and spatial factors regulating inter-kingdom communication via volatiles

Future Aspects of Modern Forensic Tools and Devices

Applying a risk assessment guided evaluation for verifying comprehensive two-dimensional gas chromatography to analyse complex pharmaceuticals

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